Method for the Production of Recombinant Peptides with a Low Amount of Trisulfides

Abstract

Methods for the production of recombinant peptides comprise fermenting cells to produce recombinant peptides. A metal salt is added to a concentrate of the fermented cells after the fermentation step, prior to peptide isolation, thereby reducing the amount of trifulsides formed in the production of the recombinant peptide. In one embodiment, the recombinant peptide is recombinant growth hormone.

1 - 10 . (canceled)  
   
   
       11 . Method for the production of recombinant peptides with a low amount of trisulfides, comprising fermenting cells to produce the recombinant peptides, wherein a metal salt is added during or after the fermentation step, prior to peptide isolation.  
   
   
       12 . Method according to  claim 11 , wherein the addition is performed directly after fermentation.  
   
   
       13 . Method according to  claim 11 , wherein pH is equal to or lower than pH 7.  
   
   
       14 . Method according to  claim 11 , wherein the metal salt is potassium or sodium salt.  
   
   
       15 . Method according to  claim 14 , in which the metal salt is potassium or sodium phosphate or acetate.  
   
   
       16 . Method according to  claim 11 , wherein the peptide is growth hormone.  
   
   
       17 . Method according to  claim 11 , wherein the metal salt is an alkali metal salt or an alkali earth metal salt.  
   
   
       18 . Method according to  claim 11 , wherein the peptide is human growth hormone.  
   
   
       19 . Method for the reduction of the amount of trisulfides in the production of recombinant peptides, comprising fermenting cells to produce recombinant peptides, wherein a metal salt is added during or after fermentation, prior to peptide isolation.  
   
   
       20 . Method according to  claim 19 , wherein the addition is performed directly after fermentation.  
   
   
       21 . Method according to  claim 19 , wherein the metal salt is an alkali metal salt or an alkali earth metal salt.  
   
   
       22 . Method according to  claim 19 , wherein pH is equal to or lower than pH 7.  
   
   
       23 . Method according to  claim 19 , wherein the metal salt is potassium or sodium salt.  
   
   
       24 . Method according to  claim 23  in which the metal salt is potassium or sodium phosphate or acetate.  
   
   
       25 . Method according to  claim 19 , wherein the peptide is growth hormone.  
   
   
       26 . Method according to  claim 19 , wherein the peptide is human growth hormone.