Transcriptional regulatory factor

Abstract

BLAST search was done on the EST database by using various nucleotide sequences encoding known bromodomain motifs to discover several ESTs likely encoding bromodomain genes. Next, testicular cDNAs were PCR cloned by using primers designed based on the sequence of EST (W17142), which is one of the ESTs discovered above. By using the thus obtained PCR product as a probe, the testicular library was screened. The obtained cDNA clone was used as a probe to re-screen the testicular cDNA library, thereby successfully isolating a full-length cDNA corresponding to EST (W17142). The protein encoded by the thus isolated cDNA had, in addition to the bromodomain, several regions and domains conserved in transcription regulatory factors. Moreover, the protein interacted with proteins associated with the chromatin-mediated transcriptional regulatory mechanism and a transcription co-activator.

1 - 31 . (canceled)  
     
     
         32 . An isolated antibody that specifically binds to a polypeptide the sequence of which consists of SEQ ID NO: 1 or 10.  
     
     
         33 . The antibody of  claim 32 , wherein the antibody is polyclonal.  
     
     
         34 . The antibody of  claim 32 , wherein the antibody is monoclonal.  
     
     
         35 . The antibody of  claim 32 , wherein the antibody is a human antibody.  
     
     
         36 . The antibody of  claim 32 , wherein the antibody is a humanized antibody.  
     
     
         37 . The antibody of  claim 32 , wherein the antibody is an IgG.  
     
     
         38 . The antibody of  claim 32 , wherein the antibody is an IgM.  
     
     
         39 . An isolated antibody that specifically binds to a polypeptide the sequence of which consists of SEQ ID NO:1 or 10 with up to 50 conservative amino acid substitutions.  
     
     
         40 . The antibody of  claim 39 , wherein the number of conservative amino acid substitutions is up to 30.  
     
     
         41 . The antibody of  claim 39 , wherein the number of conservative amino acid substitutions is up to 10.  
     
     
         42 . The antibody of  claim 39 , wherein the number of conservative amino acid substitutions is up to 3.  
     
     
         43 . The antibody of  claim 39 , wherein the antibody is polyclonal.  
     
     
         44 . The antibody of  claim 39 , wherein the antibody is monoclonal.  
     
     
         45 . The antibody of  claim 39 , wherein the antibody is a human antibody.  
     
     
         46 . The antibody of  claim 39 , wherein the antibody is a humanized antibody.  
     
     
         47 . The antibody of  claim 39 , wherein the antibody is an IgG.  
     
     
         48 . The antibody of  claim 39 , wherein the antibody is an IgM.