Endophytic streptomycetes from higher plants with biological activity

Abstract

The present invention relates to isolated strains of a Streptomyces spp. which are endophytes of dicotyledonous plants and to methods for selecting such strains. The present invention also relates to compounds having biological activity selected from the group consisting of munumbicin A, munumbicin B, munumbicin C and munumbicin D, kakadumycin A, kakadumycin B, and kakadumycin C. The present invention further relates to compositions of such compounds and to methods of protecting plants against attack by a plant pathogen and methods of inhibiting bacterial growth, fungal growth, viral infection, growth of parasitic organisms, and cancer cell growth with such compositions.

1 - 12 . (canceled)  
   
   
       13 . A crude extract obtained by cultivating an isolated strain of a  Streptomyces  spp. which is an endophyte of a  Kennedia nigriscans  plant stem wherein the strain is isolated  Streptomyces munumbi  NRRL 30562 and extracting the crude extract therefrom.  
   
   
       14 - 56 . (canceled)  
   
   
       57 . A composition comprising a suitable carrier and the crude extract of  claim 13 .  
   
   
       58 . A composition comprising the crude extract of  claim 13  affixed to a substrate.  
   
   
       59 . The crude extract of  claim 13  with biological activity against a  Plasmodium  spp.  
   
   
       60 . The crude extract of  claim 13  with biological activity against  Plasmodium falciparum.    
   
   
       61 . The crude extract of  claim 13  with biological activity against a bacterial pathogen.  
   
   
       62 . The crude extract of  claim 61 , wherein the bacterial pathogen is selected from the group consisting of  Escherichia coli, Shigella dysenteriae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Pseudomonas syringae, Burkholderia cepacia, Acinetobacter boumanii, Neisseria gonorrhoeae, Haemophilus influenzae, Stenotrophomas maltophilia, Staphylococcus  spp.,  Staphylococcus aureus, Staphylococcus aureus  MRSA,  Staphylococcus aureus  GISA,  Streptococcus pneumoniae, Enterococcus  spp.,  Enterococcus faecalis, Enterococcus faecalis  VRE,  Enterococcus faecium, Mycobacterium  spp.,  Mycobacterium tuberculosis, Bacillus anthracis, Erwinia carotovora, Vibrio fischeri, Streptococcus  spp., and  Acinetobacter  spp.  
   
   
       63 . The crude extract of  claim 13  having biological activity against a fungal pathogen.  
   
   
       64 . The crude extract of  claim 63 , wherein the fungal pathogen is selected from the group consisting of  Pythium ultimum, Rhizoctonia solani, Aspergillus  spp.,  Aspergillus fumigatus, Fusarium oxysporum, Botrytis alli, Alternaria helianthi, Phytophthora infestans, Penicillum  sp.,  Sclerotinia sclerotiorum, Cryptococcus neoformans, Histoplama capsulatum, Blastomyces dermatitidis, Cochliobolus carbonum, Geotrichum candidum Phytophthora cinnamomi, Rhizoctonia solani, Candida tropicalis, Candida globrata  and  Candida albicans, Candida tropicalis, Candida parapsilosis.    
   
   
       65 . The crude extract of  claim 13 , having biological activity against a viral pathogen.  
   
   
       66 . The crude extract of  claim 13 , having biological activity against cancer cells.  
   
   
       67 . The crude extract of  claim 13 , having biological activity against a plant pathogen.