US2007275411A1PendingUtilityA1

Silane mixtures

60
Assignee: MCGALL GLENN HPriority: May 25, 2006Filed: May 25, 2006Published: Nov 29, 2007
Est. expiryMay 25, 2026(expired)· nominal 20-yr term from priority
Inventors:Glenn McgallRobert G. Kuimelis
C08G 77/16C09D 183/06B01J 2219/00675B01J 2219/00432B01J 2219/00434B01J 2219/00677B01J 2219/00711B01J 2219/00729C08G 77/26B01J 2219/00605B01J 2219/00725B01J 2219/00439C09D 183/08B01J 2219/00596B01J 19/0046B01J 2219/00722C40B 50/18B01J 2219/00612B01J 2219/00497B01J 2219/00576B82Y 30/00
60
PatentIndex Score
0
Cited by
0
References
0
Claims

Abstract

Silanation compositions containing a mixture of two or more silanation reagents, where at least one silanation reagent includes a functional group capable of supporting polymer synthesis and at least one silanation reagent includes no functional group capable of supporting polymer synthesis are useful in modulating the active site density and hydrolytic stability of a surface. These compositions are particularly useful in silanating a surface prior to preparation of a polymer array and provide for increased hybridization results.

Claims

exact text as granted — not AI-modified
1 - 34 . (canceled) 
     
     
         35 . A method of preparing an array of polymers comprising:
 providing a substrate;   contacting a silica or other silicon oxide-containing surface of the substrate with a silanation composition that comprises a mixture of two or more silanation reagents, where at least one silanation reagent includes a functional group capable of supporting polymer synthesis and at least one silanation reagent includes no functional group capable of supporting polymer synthesis;   protecting the functional group capable of supporting polymer synthesis with a protecting group or reacting the functional group capable of supporting polymer synthesis with a compound having a reactive group protected by a protecting group;   removing the protecting group in selected regions of the surface to provide an exposed functional or reactive group;   reacting said exposed group with a monomer, wherein the monomer is coupled to the exposed group; and   repeating the steps of removing and reacting to produce the array of polymers.   
     
     
         36 . A method of preparing an array of polymers according to  claim 35  wherein the polymer is a nucleic acid 
     
     
         37 . A method of preparing an array of polymers according to  claim 35  wherein the polymer is a polypeptide. 
     
     
         38 . A method of preparing an array of polymers according to  claim 35  wherein the silanation composition comprises bis(3-trimethoxysilylpropyl)methylamine and one of N-2-hydroxyethyl-N-methyl-3-aminopropyltrimethoxysilane and N-2-hydroxyethyl-N,N-bis(trimethoxysilylpropyl)amine. 
     
     
         39 . A method of preparing an array of polymers according to  claim 35  wherein the silanation composition comprises bis(3-trimethoxysilylpropyl)methylamine and N-2-hydroxyethyl-N-methyl-3-aminopropyltrimethoxysilane. 
     
     
         40 . A method of preparing an array of polymers according to  claim 35  wherein the silanation composition comprises bis(3-trimethoxysilylpropyl)methylamine and N-2-hydroxyethyl-N,N-bis(trimethoxysilylpropyl)amine. 
     
     
         41 . A method of preparing an array of polymers according to  claim 35 , wherein the protecting group is a photoremovable protecting group. 
     
     
         42 . A method of preparing an array of polymers according to  claim 35 , wherein the protecting group is an acid labile protecting group. 
     
     
         43 . A method of preparing an array of polymers according to  claim 35 , wherein the step of removing comprises activating a photoacid generator in the selected regions by selective application of light having a predetermined wavelength to provide an acid and exposing the protecting group to the acid. 
     
     
         44 . A method of preparing an array of polymers according to  claim 35  wherein the at least one silanation reagent which includes a functional group capable of supporting polymer synthesis is selected from the group consisting of:
 N-(3-(triethoxysilyl)propyl)-4-hydroxybutyramide   
       
         
           
           
               
               
           
         
         N,N-bis(2-hydroxyethyl)-3-aminopropylthiethoxysilane 
       
       
         
           
           
               
               
           
         
         3-((2-hydroxyethyl)methylamino)propyltrimethoxysilane 
       
       
         
           
           
               
               
           
         
         N-(2-hydroxyethyl)-N,N-bis(trimethoxysilylpropyl)amine 
       
       
         
           
           
               
               
           
         
         1,2-bis((2-hydroxyethyl)(3-trimethoxysilylpropyl)aminoethane 
       
       
         
           
           
               
               
           
         
         3-acetoxypropyltrimethoxysilane 
       
       
         
           
           
               
               
           
         
         (trimethoxysiyloxy)-(C 3 -C 12 -alkyl)-(triethoxysilane) 
       
       
         
           
           
               
               
           
         
         3-glycidoxypropyltriethoxysilane 
       
       
         
           
           
               
               
           
         
       
       and wherein the at least one silane reagent which does not include a functional group capable of supporting polymer synthesis is selected from the group consisting of alkyltrimethoxysilane, 1,2-bis(trinethoxysilyl)ethane, 1,3-bis(trimethoxysilyl)propane, 1,6-bis(trimethoxysilyl)hexane, 3-(N,N-dimethylamino)propyltrimethoxysilane and N,N-bis(3-trimethoxysilylpropyl)methylarnine. 
     
     
         45 . A method of preparing an array of polymers according to  claim 35  wherein the at least one silanation reagent which includes a functional group capable of supporting polymer synthesis comprises a masked silanation reagent. 
     
     
         46 . A method of preparing an array of polymers according to  claim 45  wherein the masked silanation reagent is selected from the group consisting of:
 3-acetoxypropyltrimethoxysilane   
       
         
           
           
               
               
           
         
         (trimethoxysilyloxy)-(C 3 -C 12 -alkyl)-(tiethoxysilane), and 
       
       
         
           
           
               
               
           
         
         3-glycidoxypropyltriethoxysilane 
       
       
         
           
           
               
               
           
         
       
     
     
         47 . A method of preparing an array of polymers according to  claim 45  wherein said step of contacting a silica or other silicon oxide-containing surface comprises vapor deposition of the masked silanating reagent. 
     
     
         48 . A method of preparing an array of polymers according to  claim 45  further comprising the step of deprotecting the masked silanating reagent to provide a deprotected functional group capable of supporting polymer synthesis. 
     
     
         49 . A method of preparing an array of polymers according to  claim 35  wherein said silanation composition comprises a mixture of two or more silanation reagents, wherein the at least one silanation reagent which includes a functional group capable of supporting polymer synthesis is N,N-bis(2-hydroxyethyl)-3-aminopropyltriethoxysilane and the at least one silanation reagent which includes no functional group capable of supporting polymer synthesis is 1,2-bis(trimethoxysilyl)ethane 
     
     
         50 . A method of preparing an array of polymers according to  claim 35  wherein the at least one silanation reagent which includes a functional group capable of supporting polymer synthesis is N-(2-hydroxyethyl)-N,N-bis(trimethoxysilylpropyl)amine and the at least one silanation reagent which includes no functional group capable of supporting polymer synthesis is 1,2-bis(trimethoxysilyl)ethane. 
     
     
         51 . A method of preparing an array of polymers according to  claim 35  wherein the at least one silanation reagent which includes no functional group capable of supporting polymer synthesis and at the least one silanation reagent which includes a functional group capable of supporting polymer synthesis synthesis are present at predetermined ratios. 
     
     
         52 . A method of preparing an array of polymers according to  51  wherein said predetermined ratios are molar ratios. 
     
     
         53 . A method of preparing an array of polymers according to  claim 52  wherein the molar ratio of the at least one silanation reagent which includes no functional group capable of supporting polymer synthesis and the at least one silanation reagent which includes a functional group capable of supporting polymer synthesis are present at ranges from about 5 to 1 to 500 to 1, respectively. 
     
     
         54 . A method of preparing an array of polymers according to  claim 53  wherein the molar ratio ranges from about 5 to 1 to 200 to 1, respectively. 
     
     
         55 . A method of preparing an array of polymers according to  claim 54  wherein the ratio ranges from about 50 to 1 to 100 to 1, respectively. 
     
     
         56 . A method of preparing an array of polymers according to  claim 55  wherein the ratio is about 99 to 1, respectively. 
     
     
         57 . A method of preparing an array of polymers according to claim  33  wherein the at least one silanation reagent which includes no functional group capable of supporting polymer synthesis is 1,2-bis(trimethoxysilyl)ethane and the at least one silanation reagent which includes a functional group capable of supporting polymer synthesis is bis(2-hydroxyethyl)-3-aminopropyltriethoxysilane. 
     
     
         58 . A method of preparing an array of polymers according to  claim 57  wherein the molar ratio of 1,2-bis(trimethoxysilyl)ethane to bis(2-hydroxyethyl)-3-aminopropyltriethoxysilane ranges from about 5 to 1 to 500 to 17 respectively. 
     
     
         59 . A method of preparing an array of polymers according to  claim 58  wherein the molar ratio of 1,2-bis(trimethoxysilyl)ethane to bis(2-hydroxyethyl)-3-aminopropyltriethoxysilane ranges from about 5 to 1 to 200 to 1, respectively. 
     
     
         60 . A method of preparing an array of polymers according to  claim 59  wherein the molar ratio of 1,2-bis(trimethoxysilyl)ethane to bis(2-hydroxyethyl)-3-aminopropyltriethoxysilane ranges from about 50 to 1 to 100 to 1, respectively. 
     
     
         61 . A method of preparing an array of polymers according to  claim 60  wherein the molar ratio of 1,2-bis(trimethoxysilyl)ethane to bis(2-hydroxyethyl)-3-aminopropyltriethoxysilane ratio is about 99 to 1, respectively. 
     
     
         62 . A method of preparing an array of polymers according to  claim 35  wherein the composition does not comprise a mixture of bis(2-hydroxyethyl)-3-aminopropyltriethoxysilane and 1,2-bis(trimethoxysilyl)ethane. 
     
     
         63 . A method of preparing an array of polymers according to  claim 35  wherein the composition does not contain 1,2-bis(trimethoxysilyl)ethane. 
     
     
         64 - 88 . (canceled)

Cited by (0)

No later patents cite this yet.

References (0)

No backward citations on record.