Chimeric IgE Polypeptides and Host Cells

Abstract

The invention provides methods and materials related to expressing chimeric IgE proteins. Specifically, the invention provides nucleic acid vectors, host cells, and methods for producing chimeric IgE polypeptides.

1 . A clonal cell line expressing a chimeric IgE polypeptide.  
     
     
         2 . The clonal cell line of  claim 1 , wherein said cell line is a JM109, DH5α, NS0, HeLa, BHK-21, COS-7, Sf9, or CHO cell line.  
     
     
         3 . The clonal cell line of  claim 1 , wherein said cell line is a CHO cell line.  
     
     
         4 . The clonal cell line of  claim 1 , wherein said chimeric IgE polypeptide comprises a human IgE CH3 domain.  
     
     
         5 . The clonal cell line of  claim 1 , wherein said cell line comprises a nucleic acid comprising a cytomegalovirus promoter, an immunoglobulin (Ig) leader sequence, an insert sequence, and a SV40 late polyadenylation sequence, wherein said cytomegalovirus promoter is upstream from and operably linked to said insert sequence, said Ig leader sequence is downstream from said cytomegalovirus promoter and upstream from and operably linked to said insert sequence, said SV40 late polyadenylation sequence is downstream from and operably linked to said insert sequence, and said insert sequence encodes said chimeric IgE polypeptide.  
     
     
         6 . The clonal cell line of  claim 1 , wherein said chimeric IgE polypeptide has the amino acid sequence set forth in SEQ ID NO:13.