Peptides and Derivatives Thereof Showing Cell Attachment, Spreading and Detachment Activity

Abstract

The present invention relates to peptides and derivatives thereof showing cell attachment, spreading and detachment activity. Particularly, the present invention relates to the peptide NKDIL and EPDIM and derivatives thereof which promote the cell attachment activity through interaction with α 3β 1 integrin as a functional cell receptor and include aspartic acid and isoleucine essential for cell attachment and detachment activity. The peptides and derivatives thereof in the present invention can be used for developing a study of cell attachment activity mediated through various extracellular matrix protein containing β ig-h3, wound healing, tissue regeneration and metastasis inhibition.

1 . A peptide and its derivatives having cell attachment, spreading and detachment activity, comprising an amino acid sequence represented by XXDIX.  
     
     
         2 . The peptide and its derivatives according to  claim 1 , wherein the peptide and its derivatives have an amino acid sequence consisting of at least five amino acid residues represented by XXDIX, comprising aspartic acid and isoleucine as a conserved sequence.  
     
     
         3 . The peptide and its derivatives according to  claim 2 , wherein the amino acid sequence is represented by NKDIL of SEQ ID NO:2.  
     
     
         4 . The peptide and its derivatives according to  claim 2 , wherein the amino acid sequence is represented by EPDIM of SEQ ID NO:3.  
     
     
         5 . The peptide and its derivatives according to  claim 1 , wherein the peptide and its derivatives mediate cell attachment and spreading activity through α3β1 integrin.  
     
     
         6 . The peptide and its derivatives according to  claim 1 , wherein the peptide and its derivatives show inhibitory activity against cell adhesion to βig-h3, fibronectin or laminin.  
     
     
         7 . Pharmaceutical compositions, comprising the peptide or its derivatives of  claim 1  as a pharmaceutically active ingredient.  
     
     
         8 . The Pharmaceutical compositions according to  claim 7 , wherein the pharmaceutical compositions are therapeutically effective for wound healing, tissue regeneration, or cancer metastasis resistance improving biointerface of biomaterials and tissue implants.