Antibodies, polypeptides and uses thereof

Abstract

A method of inhibiting angiogenesis in an individual in need thereof comprising administering an antibody that selectively binds to the extracellular region of human magic roundabout (MR) to the individual. An antibody that has the amino acid sequences i) to iii), the amino acid sequences iv) to vi), or the amino acid sequences i) to vi): i) S A S S S V S Y M Y ii) L T S N L A S iii) Q Q W S S N P L T iv) D Y N L N v) V I N P N Y G T T S Y N Q K F K G vi) G R D Y F G Y. A method of inhibiting angiogenesis in an individual in need thereof comprising administering the extracellular domain (residues 1-467) of MR, or a fragment thereof that inhibits angiogenesis, to the individual. A method of inhibiting endothelial cell migration and/or proliferation comprising administering the extracellular domain of MR, or a fragment thereof that inhibits endothelial cell migration and/or proliferation.

1 .- 46 . (canceled)  
     
     
         47 . A method of combating a disease or condition selected from tumours/cancer, psoriasis, atherosclerosis, menorrhagia, endometriosis, arthritis (both inflammatory and rheumatoid), macular degeneration, Paget's disease, retinopathy and its vascular complications (including proliferative and of prematurity, and diabetic retinopathy), benign vascular proliferations, fibroses, obesity and inflammation in an individual in need thereof comprising 
 administering an antibody that selectively binds to the extracellular region of magic roundabout (MR) to the individual.    
     
     
         48 .- 52 . (canceled)  
     
     
         53 . A method of combating a disease or condition involving unwanted, undesirable or inappropriate endothelial cell migration and/or proliferation in an individual, the method comprising 
 administering a magic roundabout (MR) ectodomain or a fragment thereof that inhibits endothelial cell migration and/or proliferation, or a polynucleotide encoding a MR ectodomain or a fragment thereof that inhibits endothelial cell migration and/or proliferation, to the individual.    
     
     
         54 . (canceled)  
     
     
         55 . A method according to  claim 53 , wherein the disease or condition involving unwanted, undesirable or inappropriate endothelial cell migration and/or proliferation is selected from tumours/cancer, psoriasis, atherosclerosis, menorrhagia, endometriosis, arthritis (both inflammatory and rheumatoid), macular degeneration, Paget's disease, retinopathy and its vascular complications (including proliferative and of prematurity, and diabetic retinopathy), benign vascular proliferations, fibroses, obesity or inflammation.  
     
     
         56 . An in vitro method of inhibiting endothelial cell migration and/or proliferation comprising 
 administering a magic roundabout (MR) ectodomain or a fragment thereof that inhibits endothelial cell migration and/or proliferation, a polynucleotide encoding a MR ectodomain or a fragment thereof that inhibits endothelial cell migration and/or proliferation, to tissue or cells in vitro.    
     
     
         57 .- 58 . (canceled)  
     
     
         59 . The MR ectodomain, or a fragment thereof of  claim 56  that inhibits angiogenesis.  
     
     
         60 . A method of inhibiting angiogenesis in an individual in need thereof comprising administering a magic roundabout (MR) ectodomain, or a fragment thereof that inhibits angiogenesis, to the individual.  
     
     
         61 .- 73 . (canceled)