Mushroom extracts having anticancer activity

Abstract

The invention relates to a method for treatment of chronic myelogenous leukemia (CML), Ph+ acute lymphoblastic leukemia (ALL), prostate cancer and β-globin disorders such as sickle cell anemia and β-thalassemia, comprising administration of a mycelium extract from at least one higher Basidiomycetes medicinal mushroom selected from the group consisting of Ganoderma adspersum, Hypsizygus ulmarium, Kuehneromyces mutabilis, Omphalotus olearius, Panus conchatus, Piptoporus betulinus, Pleurotus eryngii, and Trametes zonata.

1 . A method for treatment of prostate cancer, comprising administering to a patient in need thereof a therapeutically effective amount of a mycelium extract from the medicinal mushroom  Trametes zonata,  wherein said mycelium extract is obtained by extraction of a dry mycelium of said mushroom with an extraction solvent comprising one or more organic solvents selected from the group consisting of methanol, ethanol, acetonitrile, ethyl acetate, chloroform, hexane, cyclohexane, isooctane and dichloromethane.  
   
   
       2 . A method for treatment of a disease selected from the group consisting of a Philadelphia Chromosome-positive Leukemia, prostate cancer, and a β-globin disorder, comprising administering to a patient in need thereof a therapeutically effective amount of a mycelium extract from at least one higher  Basidiomycetes  medicinal mushroom selected from the group consisting of  Ganoderma adspersum, Hypsizygus ulmarium, Kuehneromyces mutabilis, Omphalotus olearius, Panus conchatus, Piptoporus betulinus, Pleurotus eryngii,  and  Trametes zonata,  wherein said mycelium extract is obtained by extraction of a dry mycelium of said mushroom with an extraction solvent comprising one or more organic solvents selected from the group consisting of methanol, ethanol, acetonitrile, ethyl acetate, chloroform, hexane, cyclohexane, isooctane and dichloromethane.  
   
   
       3 . The method according to  claim 2 , wherein said extraction solvent is a non-aqueous organic solvent.  
   
   
       4 . The method according to  claim 3 , wherein said organic solvent is selected from the group consisting of methanol, ethanol, dichloromethane and ethyl acetate.  
   
   
       5 . The method according to  claim 2 , wherein said extraction solvent is an aqueous organic solvent.  
   
   
       6 . The method according to  claim 5 , wherein said extraction solvent is 70% ethanol or a mixture of ethyl acetate and methanol.  
   
   
       7 . The method according to  claim 2 , wherein said Philadelphia Chromosome-positive Leukemia is chronic myelogenous leukemia (CML) or Ph+ acute lymphoblastic leukemia (ALL).  
   
   
       8 . The method according to  claim 2 , wherein said mycelium extract is further concentrated and purified to obtain a concentrated and purified mycelium extract.  
   
   
       9 . The method according to  claim 8 , wherein the concentrated mycelium extract is purified by column chromatography, fractional distillation, preparative thin layer chromatography (TLC), preparative high performance liquid chromatography (HPLC), or centrifugal partition chromatography (CPC).