Telomelysin/gfp-expressing recombinant virus

Abstract

The present invention relates to providing a reagent for cancer cell detection or cancer diagnosis, a pharmaceutical composition for the treatment of cancer, and a method of treating or preventing cancer in a subject. Specifically, the present invention relates to a reagent for cancer cell detection, comprising a recombinant virus where a replication cassette comprising a promoter from human telomerase, an E1A gene, an IRES sequence and an E1B gene in this order is integrated in E1 region of the viral genome and a labeling cassette comprising a gene encoding a labeling protein and a promoter capable of regulating the expression of the gene encoding the labeling protein is integrated in E3 region of the viral genome.

1 . A reagent for cancer cell detection, comprising a recombinant virus where a replication cassette comprising a promoter from human telomerase, an E1A gene, an IRES sequence and an E1B gene in this order is integrated in E1 region of the viral genome and a labeling cassette comprising a gene encoding a labeling protein and a promoter capable of regulating the expression of the gene encoding the labeling protein is integrated in E3 region of the viral genome.  
     
     
         2 . A reagent for cancer diagnosis, comprising a recombinant virus where a replication cassette comprising a promoter from human telomerase, an E1A gene, an IRES sequence and an E1B gene in this order is integrated in E1 region of the viral genome and a labeling cassette comprising a gene encoding a labeling protein and a promoter capable of regulating the expression of the gene encoding the labeling protein is integrated in E3 region of the viral genome.  
     
     
         3 - 5 . (canceled)  
     
     
         6 . The reagent according to  claim 1 , wherein the promoter capable of regulating the expression of the gene encoding a labeling protein is a cytomegalovirus promoter or hTERT promoter.  
     
     
         7 . (canceled)  
     
     
         8 . A cell death-inducing agent, comprising a recombinant virus where a replication cassette comprising a promoter from human telomerase, an E1A gene, an IRES sequence and an E1B gene in this order is integrated in E1 region of the viral genome and a cell death-inducing cassette comprising a gene encoding a protein associated with cell death induction and a promoter capable of regulating the expression of the gene encoding the protein is integrated in E3 region of the viral genome.  
     
     
         9 - 13 . (canceled)  
     
     
         14 . The cell death-inducing agent according to  claim 8 , wherein the promoter capable of regulating the gene encoding the protein associated with cell death induction is a cytomegalovirus promoter or hTERT promoter.  
     
     
         15 - 19 . (canceled)  
     
     
         20 . A method of treating or preventing cancer in a subject, comprising administering to the subject a pharmaceutical composition comprising a recombinant virus where a replication cassette comprising a promoter from human telomerase, an E1A gene, an IRES sequence and an E1B gene in this order is integrated in E1 region of the viral genome and a labeling cassette comprising a gene encoding a labeling protein and a promoter capable of regulating the expression of the gene encoding the labeling protein is integrated in E3 region of the viral genome.  
     
     
         21 . The method according to  claim 20 , wherein the promoter from human telomerase is hTERT promoter.  
     
     
         22 . The method according to  claim 20 , wherein the labeling protein is GFP.  
     
     
         23 . The method according to  claim 20 , wherein the promoter capable of regulating the expression of the gene encoding a labeling protein is a cytomegalovirus promoter or hTERT promoter.  
     
     
         24 . The method according to  claim 20 , wherein the virus is an adenovirus.  
     
     
         25 . The reagent according to  claim 2 , wherein the promoter capable of regulating the expression of the gene encoding a labeling protein is a cytomegalovirus promoter or hTERT promoter.  
     
     
         26 . A method of detecting cancer cells, comprising: 
 a) infecting cancer cells with a reagent comprising a recombinant virus where a replication cassette comprising a promoter from human telomerase, an E1A gene, an IRES sequence and an E1B gene in this order is integrated in E1 region of the viral genome and a labeling cassette comprising a gene encoding a labeling protein and a promoter capable of regulating the expression of the gene encoding the labeling protein is integrated in E3 region of the viral genome; and    b) detecting the fluorescence emitted by the cancer cells.    
     
     
         27 . The method of  claim 26 , wherein the promoter capable of regulating the expression of the gene encoding a labeling protein is a cytomegalovirus promoter or hTERT promoter.  
     
     
         28 . A method of cancer diagnosis, comprising: 
 a) infecting cancer cells with a reagent comprising a recombinant virus where a replication cassette comprising a promoter from human telomerase, an E1A gene, an IRES sequence and an E1B gene in this order is integrated in E1 region of the viral genome and a labeling cassette comprising a gene encoding a labeling protein and a promoter capable of regulating the expression of the gene encoding the labeling protein is integrated in E3 region of the viral genome; and    b) detecting the fluorescence emitted by the cancer cells.    
     
     
         29 . The method of  claim 28 , wherein the promoter capable of regulating the expression of the gene encoding a labeling protein is a cytomegalovirus promoter or hTERT promoter.  
     
     
         30 . A method of inducing cell death in a target cell, comprising infecting the target cell with a cell death-inducing agent comprising a recombinant virus where a replication cassette comprising a promoter from human telomerase, an E1A gene, an IRES sequence and an E1B gene in this order is integrated in E1 region of the viral genome and a cell death-inducing cassette comprising a gene encoding a protein associated with cell death induction and a promoter capable of regulating the expression of the gene encoding the protein is integrated in E3 region of the viral genome.  
     
     
         31 . The method of  claim 30 , wherein the promoter capable of regulating the gene encoding the protein associated with cell death induction is a cytomegalovirus promoter or hTERT promoter.