Extract of plant Dendrobii Caulis and preparing process thereof

Abstract

An extract of plant Dendrobii Caulis and preparing process thereof are provided. A physiologically active extract of a plant Dendrobii Caulis and the method thereof are provided in the present invention. The extract is obtained by an extraction of the plant or parts thereof with a water miscible organic solvent or a mixture thereof with water.

1 . An extract of a plant  Dendrobii Caulis,  obtained by an extraction of said plant or parts thereof with a water miscible organic solvent or a mixture thereof with water.  
     
     
         2 . The extract as claimed in  claim 1 , wherein said organic solvent is one selected from a group consisting of an alcohol having 1 to 8 carbon atoms, an alkane, and an ester.  
     
     
         3 . The extract as claimed in  claim 2 , wherein said alcohol is one of methanol and ethanol.  
     
     
         4 . A physiological active composition comprising a physiologically acceptable carrier for carrying therewith, and one of an extract according to  claim 1  and an isomer of said extract.  
     
     
         5 . The composition as claimed in  claim 4 , wherein said physiological active composition is a pharmaceutical composition.  
     
     
         6 . The composition as claimed in  claim 4 , wherein said physiologically acceptable carrier is a pharmaceutical carrier.  
     
     
         7 . A process for preparing an extract from a plant  Dendrobii Caulis,  comprising plural steps of extracting said plant or parts thereof with a water, a water miscible organic solvent or a mixture thereof.  
     
     
         8 . The process as claimed in  claim 7 , wherein said organic solvent is one selected from a group consisting of an alcohol having 1 to 8 carbon atoms, an alkane, and an ester.  
     
     
         9 . The process as claimed in  claim 8 , wherein said alcohol is one of methanol and ethanol.  
     
     
         10 . A process for preparing an extract from a plant, comprising steps of: 
 a) obtaining a first alcohol extract from said plant; extracting said first alcohol extract by a water and an alkane simultaneously for obtaining a first water layer and an alkane extract;    b) extracting said first water layer by an ester for obtaining an ester extract and a second water layer; and    c) extracting said second water layer by a second alcohol for obtaining a second alcohol extract and a third water layer.    
     
     
         11 . The process as claimed in  claim 10 , wherein said plant belongs to Genus  Dendrobium.    
     
     
         12 . The process as claimed in  claim 10 , wherein said step a) further comprises steps of: 
 a1) providing a dry material of said plant;    a2) grinding said dry material by a pulverizer; and    a3) extracting said ground dry material by said first alcohol for obtaining said first alcohol extract.    
     
     
         13 . The process as claimed in  claim 10 , wherein said first alcohol is an alcohol having 1 to 8 carbon atoms.  
     
     
         14 . The process as claimed in  claim 13 , wherein said first alcohol is one of methanol and ethanol.  
     
     
         15 . The process as claimed in  claim 10 , wherein said second alcohol is an alcohol having 1 to 8 carbon atoms.  
     
     
         16 . The process as claimed in  claim 15 , wherein said second alcohol is one of butanol and n-butanol.  
     
     
         17 . The process as claimed in  claim 10 , wherein said step b) further comprises a step of: 
 b1) drying said first alcohol extract through steps of decompressing, condensing, and exhausting.    
     
     
         18 . The process as claimed in  claim 10 , wherein said alkane extract is an n-hexane extract.  
     
     
         19 . The process as claimed in  claim 10 , wherein said step c) further comprises steps of: 
 c1) drying said ester extract; and    c2) extracting said dried ester extract with a hexane and a methanol for obtaining a hexane extract and a methanol extract.    
     
     
         20 . The process as claimed in  claim 19 , wherein said hexane extract is dried by steps of decompressing, condensing, and exhausting.  
     
     
         21 . The process as claimed in  claim 10 , wherein said ester is an ethyl-acetate.  
     
     
         22 . The process as claimed in  claim 10  further comprising steps of: 
 e) chromatographing said second alcohol extract for obtaining a first eluate named as DCMPbL6,7; and    f) chromatographing said DCMPbL6,7 by a mobile phase for obtaining a second eluate.    
     
     
         23 . The process as claimed in  claim 22 , wherein said step e) is performed by an eluent of a methanol/water mixture in a 50:50 volume ratio.  
     
     
         24 . The process as claimed in  claim 22 , wherein said mobile phase is an isopropanol/water mixture in a 20:80 volume ratio, and said second eluate is named as DCMPbL6,7D2.  
     
     
         25 . The process as claimed in  claim 24 , wherein said DCMPbL6,7D2 is further chromatographed with a methanol/water/acetic acid mixture in a 35:65:1 volume ratio for obtaining a third eluate named as DCMPbL6,7D2H2.  
     
     
         26 . The process as claimed in  claim 22 , wherein said mobile phase is an isopropanol/water mixture in a 30:70 volume ratio, and said second eluate is named as DCMPbL6,7D3.  
     
     
         27 . The process as claimed in  claim 26 , wherein said DCMPbL6,7D3 is further chromatographed with a methanol/water/acetic acid mixture in a 40:60:1 volume ratio for obtaining a fourth eluate named as DCMPbL6,7D3H3.  
     
     
         28 . The process as claimed in  claim 22 , wherein said mobile phase is an isopropanol/water mixture in a 40:60 volume ratio, and said second eluate is named as DCMPbL6,7D4.  
     
     
         29 . The process as claimed in  claim 28 , wherein said DCMPbL6,7D4 is chromatographed with a methanol/water/acetic acid mixture in a 45:55:1 volume ratio for obtaining a fifth eluate named as DCMPbL6,7D4H3.  
     
     
         30 . An extract obtained according to the process of  claim 10 .  
     
     
         31 . A physiological active composition comprising a physiologically acceptable carrier for carrying therewith, and one of an extract according to  claim 30  and an isomer of said extract.  
     
     
         32 . The composition as claimed in  claim 31 , wherein said physiological active composition is a pharmaceutical composition.  
     
     
         33 . The composition as claimed in  claim 31 , wherein said physiologically acceptable carrier is a pharmaceutical carrier.  
     
     
         34 . An eluate being said second eluate obtained according to the process of  claim 22 .  
     
     
         35 . A physiological active composition comprising a physiologically acceptable carrier for carrying therewith, and one of an eluate according to  claim 34  and an isomer of said eluate.  
     
     
         36 . The composition as claimed in  claim 35 , wherein said physiological active composition is a pharmaceutical composition.  
     
     
         37 . The composition as claimed in  claim 35 , wherein said physiologically acceptable carrier is a pharmaceutical carrier.  
     
     
         37 . An eluate being said third eluate according to the process of  claim 25 .  
     
     
         38 . A physiological active composition comprising a physiologically acceptable carrier for carrying therewith, and one of an eluate according to  claim 38  and an isomer of said eluate.  
     
     
         39 . The composition as claimed in  claim 39 , wherein said physiological active composition is a pharmaceutical composition.  
     
     
         40 . The composition as claimed in  claim 39 , wherein said physiologically acceptable carrier is a pharmaceutical carrier.  
     
     
         41 . An eluate being said fourth eluate according to the process of  claim 27 .  
     
     
         42 . A physiological active composition comprising a physiologically acceptable carrier for carrying therewith, and one of an eluate according to  claim 42  and an isomer of said eluate.  
     
     
         43 . The composition as claimed in  claim 43 , wherein said physiological active composition is a pharmaceutical composition.  
     
     
         44 . The composition as claimed in  claim 43 , wherein said physiologically acceptable carrier is a pharmaceutical carrier.  
     
     
         45 . An eluate being said fifth eluate according to the process of  claim 29 .  
     
     
         46 . A physiological active composition comprising a physiologically acceptable carrier for carrying therewith, and one of an eluate according to  claim 46  and an isomer of said eluate.  
     
     
         47 . The composition as claimed in  claim 47 , wherein said physiological active composition is a pharmaceutical composition.  
     
     
         48 . The composition as claimed in  claim 47 , wherein said physiologically acceptable carrier is a pharmaceutical carrier.  
     
     
         50 . A process for preparing an extract from a plant, comprising steps of: 
 a) obtaining a first organic extract from said plant;    b) extracting said first organic extract by a water and a second organic solvent simultaneously for obtaining a first water layer and a second organic extract;    c) extracting said first water layer by a third organic solvent for obtaining a third organic extract and a second water layer; and    d) extracting said second water layer by a four organic solvent for obtaining a fourth organic extract and a third water layer.    
     
     
         51 . The process as claimed in  claim 50 , wherein said plant is an orchid.  
     
     
         52 . The process as claimed in  claim 50 , wherein said step a) further comprises steps of 
 a1) providing a dry material of said plant;    a2) grinding said dry material by a pulverizer; and    a3) extracting said ground dry material by said first organic solvent for obtaining said first alcohol extract.    
     
     
         53 . The process as claimed in  claim 50 , wherein said first organic solvent is an alcohol having 1 to 8 carbon atoms.  
     
     
         54 . The process as claimed in  claim 50 , wherein said second organic solvent is an alkane having 1 to 8 carbons.  
     
     
         55 . The process as claimed in  claim 50 , wherein said third organic solvent is an ester.  
     
     
         56 . The process as claimed in  claim 50 , wherein said fourth organic solvent is an alcohol having 1 to 8 carbon atoms.  
     
     
         57 . The process as claimed in  claim 56 , wherein said alcohol is one of butanol and n-butanol.  
     
     
         58 . A substance defined by FIGS.  5  to  11 .  
     
     
         59 . A physiological active composition comprising a physiologically acceptable carrier for carrying therewith, and one of a substance according to  claim 58  and an isomer of said substance.  
     
     
         60 . The composition as claimed in  claim 59 , wherein said physiological active composition is a pharmaceutical composition.  
     
     
         61 . The composition as claimed in  claim 59 , wherein said physiologically acceptable carrier is a pharmaceutical carrier.  
     
     
         62 . A substance defined by FIGS.  13  to  17 .  
     
     
         63 . A physiological active composition comprising a physiologically acceptable carrier for carrying therewith, and one of a substance according to  claim 62  and an isomer of said substance.  
     
     
         64 . The composition as claimed in  claim 63 , wherein said physiological active composition is a pharmaceutical composition.  
     
     
         65 . The composition as claimed in  claim 63 , wherein said physiologically acceptable carrier is a pharmaceutical carrier.  
     
     
         66 . A substance defined by FIGS.  19  to  24 .  
     
     
         67 . A physiological active composition comprising a physiologically acceptable carrier for carrying therewith, and one of a substance according to  claim 66  and an isomer of said substance.  
     
     
         68 . The composition as claimed in  claim 67 , wherein said physiological active composition is a pharmaceutical composition.  
     
     
         69 . The composition as claimed in  claim 67 , wherein said physiologically acceptable carrier is a pharmaceutical carrier.