Compositions and Methods for Controlling Insects

Abstract

The present invention comprises compositions methods and cell lines related to controlling insects. An embodiment of a composition comprises a plant essential oil and targets at least one receptor of insects chosen from tyramine receptor, Or83 b olfactory receptor, and Or43 a olfactory receptor, resulting in a change in the intracellular levels of cAMP, Ca2+, or both.

1 . A composition for controlling insects, comprising: at least two oils, wherein the composition targets at least one receptor of the insects chosen from tyramine receptor, Or83b olfactory receptor, and Or43a olfactory receptor, resulting in a change in the intracellular levels of cAMP, Ca2+, or both in said insects.  
     
     
         2 . The composition of  claim 1 , wherein the at least two oils comprise oils chosen from: t-anthole, black seed oil, camphene, carvacrol, d-carvone, l-carvone, 1,8-cineole, p-cymene, diethyl phthalate, eugenol, geraniol, isopropyl citrate, lemon grass oil, lilac flower oil, lime oil, d-limonene, linalyl anthranilate, linalool, lindenol, methyl citrate, methyl di-hydrojasmonate, myrcene, perillyl alcohol, phenyl acetaldehyde, α-pinene, β-pinene, piperonal, piperonyl, piperonyl acetate, piperonyl alcohol, piperonyl amine, pulegone, quinine, sabinine, α-terpinene, terpinene 900, α-terpineol, gamma-terpineol, 2-tert-butyl-p-quinone, α-thujone, thyme oil, and thymol.  
     
     
         3 . The composition of  claim 2 , wherein treatment with said composition has a repellent effect.  
     
     
         4 . The composition of  claim 2 , wherein treatment with said composition has a pesticidal effect.  
     
     
         5 . The composition of  claim 2 , and further comprising at least one fixed oil.  
     
     
         6 . The composition of  claim 5 , wherein the at least one fixed is an oil chosen from: castor oil, corn oil, cumin oil, mineral oil, olive oil, peanut oil, safflower oil, sesame oil, and soy bean oil.  
     
     
         7 . The composition of  claim 2 , wherein the oils chosen comprise: lilac flower oil; geraniol, and thyme oil.  
     
     
         8 . The composition of  claim 3 , wherein the oils comprise: lilac flower oil; geraniol, and thyme oil.  
     
     
         9 . The composition of  claim 6 , wherein the oils chosen comprise: lilac flower oil, geraniol, thyme oil, and cumin oil.  
     
     
         10 . The composition of  claim 9 , wherein the oils chosen comprise: about 44% by weight lilac flower oil, about 10% by weight geraniol, about 2% by weight thyme oil, and about 44% by weight cumin oil.  
     
     
         11 . The composition of  claim 1 , wherein the oils chosen comprise: d-limonene, thyme oil, geraniol, α-pinene, and p-cymene.  
     
     
         12 . The composition of  claim 3 , wherein the oils chosen comprise: d-limonene, thyme oil, geraniol, α-pinene, and p-cymene.  
     
     
         13 . The composition of  claim 11 , wherein the oils chosen comprise: about 20% by weight d-limonene, about 20% by weight thyme oil, about 20% by weight geraniol, about 20% by weight α-pinene, and about 20% by weight p-cymene.  
     
     
         14 . The composition of  claim 11 , and further comprising phenylacetaldehyde.  
     
     
         15 . The composition of  claim 12 , and further comprising phenylacetaldehyde.  
     
     
         16 . The composition of  claim 14 , wherein the oils chosen comprise: about 10% by weight d-limonene, about 30% by weight thyme oil, about 35% by weight geraniol, about 10% by weight α-pinene, and about 5% by weight phenylacetaldehyde.  
     
     
         17 . The composition of  claim 2 , wherein the oils chosen comprise: geraniol, thyme oil, and lemon grass oil.  
     
     
         18 . The composition of  claim 3 , wherein the oils chosen comprise: geraniol, thyme oil, and lemon grass oil.  
     
     
         19 . The composition of  claim 17 , wherein the oils chosen comprise: about 50% by weight geraniol, about 40% by weight thyme oil, and about 10% by weight lemon grass oil.  
     
     
         20 . The composition of  claim 2 , wherein the oils chosen comprise: d-limonene, lindenol, eugenol, phenylacetaldehyde, and geraniol.  
     
     
         21 . The composition of  claim 3 , wherein the oils chosen comprise: d-limonene, lindenol, eugenol, phenylacetaldehyde, and geraniol.  
     
     
         22 . The composition of  claim 20 , wherein the oils chosen comprise: about 20% by weight d-limonene, about 10% by weight lindenol, about 10% by weight eugenol, about 10% by weight phenylacetaldehyde, and about 50% by weight geraniol.  
     
     
         23 . The composition of  claim 2 , wherein the oils chosen comprise: d-limonene, geraniol, α-pinene, p-cymene, and phenylacetaldehyde.  
     
     
         24 . The composition of  claim 3 , wherein the oils chosen comprise: d-limonene, geraniol, α-pinene, p-cymene, and phenylacetaldehyde.  
     
     
         25 . The composition of  claim 23 , wherein the oils chosen comprise: about 15% by weight d-limonene, about 50% by weight geraniol, about 15% by weight α-pinene, about 15% by weight p-cymene, and about 5% by weight phenylacetaldehyde.  
     
     
         26 . The composition of  claim 2 , wherein the oils chosen comprise: d-limonene and p-cymene.  
     
     
         27 . The composition of  claim 2 , wherein the oils chosen comprise: d-limonene, p-cymene, and α-pinene.  
     
     
         28 . The composition of  claim 2 , wherein the oils chosen comprise: d-limonene and α-pinene.  
     
     
         29 . The composition of  claim 2 , wherein the oils chosen comprise: d-limonene and thyme oil.  
     
     
         30 . The composition of  claim 2 , wherein the oils chosen comprise: α-pinene and thyme oil.  
     
     
         31 . The composition of  claim 2 , wherein the oils chosen comprise: p-cymene, α-pinene and thyme oil.  
     
     
         32 . The composition of  claim 2 , wherein the oils chosen comprise: p-cymene and α-pinene.  
     
     
         33 . The composition of  claim 2 , wherein the oils chosen comprise: linalool, p-cymene, thyme oil, and α-pinene.  
     
     
         34 . The composition of  claim 2 , wherein the oils chosen comprise: linalool, p-cymene, and α-pinene.  
     
     
         35 . The composition of  claim 2 , wherein the oils chosen comprise: d-limonene, p-cymene, and thymol.  
     
     
         36 . The composition of  claim 2 , wherein the oils chosen comprise: d-limonene, p-cymene, thymol and geraniol.  
     
     
         37 . The composition of  claim 2 , wherein the oils chosen comprise: α-thujone, α-pinene, sabinene, β-pinene, p-cymene and limonene.  
     
     
         38 . The composition of  claim 3 , wherein the oils chosen comprise: α-thujone, α-pinene, sabinene, β-pinene, p-cymene and limonene.  
     
     
         39 . The composition of  claim 2 , wherein the oils chosen comprise: diethyl phthalate, α-terpineol, piperonal, linalool, gamma-terpineol, methyl di-hydrojasmonate, methyl citrate and isopropyl citrate.  
     
     
         40 . The composition of  claim 3 , wherein the oils chosen comprise: diethyl phthalate, α-terpineol, piperonal, linalool, gamma-terpineol, methyl di-hydrojasmonate, methyl citrate and isopropyl citrate.  
     
     
         41 . The composition of  claim 2 , wherein the oils chosen comprise at least two oils chosen from: d-limonene and p-cymene, α-pinene, thyme oil, linalool, thymol, and geraniol.  
     
     
         42 . The composition of  claim 3 , wherein the oils chosen comprise at least two oils chosen from: d-limonene and p-cymene, α-pinene, thyme oil, linalool, thymol, and geraniol.  
     
     
         43 . The composition of  claim 2 , wherein the composition comprises at least two oils chosen from: d-limonene, p-cymene, α-pinene, thyme oil, thymol, geraniol, lemon grass oil, black seed oil, lilac flower oil, mineral oil, and phenylacetaldehyde.  
     
     
         44 . The composition of  claim 3 , wherein the composition comprises at least two oils chosen from: d-limonene, p-cymene, α-pinene, thyme oil, thymol, geraniol, lemon grass oil, black seed oil, lilac flower oil, mineral oil, and phenylacetaldehyde.  
     
     
         45 . The composition of  claim 1 , wherein the at least two oils are chosen from: black seed oil, camphene, d-carvone, l-carvone, 1,8-cineole, isopropyl citrate, lemon grass oil, lilac flower oil, lime oil, linalyl anthranilate, methyl citrate, methyl di-hydrojasmonate, myrcene, perillyl alcohol, phenyl acetaldehyde, α-pinene, β-pinene, piperonyl, piperonyl amine, quinine, sabinine, α-terpinene, terpinene 900, gamma-terpineol, 2-tert-butyl-p-quinone, and α-thujone.  
     
     
         46 . The composition of  claim 45 , and further comprising at least one fixed oil.  
     
     
         47 . The composition of  claim 46 , wherein the at least one fixed oil comprises an oil chosen from: castor oil, corn oil, cumin oil, mineral oil, olive oil, peanut oil, safflower oil, sesame oil, and soy bean oil.  
     
     
         48 . The composition of  claim 47 , wherein the oils chosen comprise: lilac flower oil and cumin oil.  
     
     
         49 . A composition for controlling insects, comprising at least one oil chosen from: black seed oil and lilac flower oil.  
     
     
         50 . The composition of  claim 49 , and further comprising at least one plant essential oil.  
     
     
         51 . The composition of  claim 50 , wherein the at least one plant essential oil comprises an oil chosen from: t-anthole, black seed oil, camphene, carvacrol, d-carvone, l-carvone, 1,8-cineole, p-cymene, diethyl phthalate, eugenol, geraniol, isopropyl citrate, lemon grass oil, lilac flower oil, lime oil, d-limonene, linalyl anthranilate, linalool, lindenol, methyl citrate, methyl di-hydrojasmonate, myrcene, perillyl alcohol, phenyl acetaldehyde, α-pinene, β-pinene, piperonal, piperonyl, piperonyl acetate, piperonyl alcohol, piperonyl amine, quinine, sabinine, α-terpinene, terpinene 900, α-terpineol, gamma-terpineol, 2-tert-butyl-p-quinone, α-thujone, thyme oil, thymol, castor oil, corn oil, cumin oil, mineral oil, olive oil, peanut oil, safflower oil, sesame oil, and soy bean oil.  
     
     
         52 . The composition of  claim 51 , wherein the oils chosen comprise: lilac flower oil and cumin oil.  
     
     
         53 . The composition of  claim 51 , wherein the oils chosen comprise: lilac flower oil; geraniol, and thyme oil.  
     
     
         54 . The composition of  claim 53 , wherein the oils chosen comprise: lilac flower oil, geraniol, thyme oil, and cumin oil.  
     
     
         55 . A composition for controlling insects, comprising: 
 at least one plant essential oil; and    an insect control agent, wherein the final concentration of said insect control agent is lower than required to exhibit insect control activity when said insect control agent is used alone.    
     
     
         56 . The composition of  claim 55 , wherein the at least one plant essential oil comprises at least one oil chosen from: t-anthole, black seed oil, camphene, carvacrol, d-carvone, l-carvone, 1,8-cineole, p-cymene, diethyl phthalate, eugenol, geraniol, isopropyl citrate, lemon grass oil, lilac flower oil (LFO), lime oil, d-limonene, linalyl anthranilate, linalool, lindenol, methyl citrate, methyl di-hydrojasmonate, myrcene, perillyl alcohol, phenyl acetaldehyde, α-pinene, β-pinene, piperonal, piperonyl, piperonyl acetate, piperonyl alcohol, piperonyl amine, pulegone, quinine, sabinine, α-terpinene, terpinene 900, α-terpineol, gamma-terpineol, 2-tert-butyl-p-quinone, α-thujone, thyme oil, and thymol.  
     
     
         57 . The composition of  claim 56 , wherein the insect control agent is DEET.  
     
     
         58 . The composition of  claim 55 , wherein the insect control agent is DEET.  
     
     
         59 . The composition of  claim 58 , wherein the final concentration of DEET is at least as low as 10%.  
     
     
         60 . The composition of  claim 59 , wherein the final concentration of DEET is at least as low as 5%.  
     
     
         61 . The composition of  claim 57 , and further comprising a fixed oil.  
     
     
         62 . The composition of  claim 61 , wherein the fixed oil comprises an oil chosen from: castor oil, corn oil, cumin oil, mineral oil, olive oil, peanut oil, safflower oil, sesame oil, and soy bean oil.  
     
     
         63 . The composition of  claim 62 , wherein the oils chosen comprise: DEET, lilac flower oil, and cumin oil.  
     
     
         64 . The composition of  claim 63 , wherein the oils chosen comprise: about 10% by weight DEET, about 45% by weight lilac flower oil and about 45% by weight cumin oil.  
     
     
         65 . The composition of  claim 57 , wherein the oils chosen comprise: DEET, d-limonene, thyme oil, geraniol, α-pinene, and p-cymene.  
     
     
         66 . The composition of  claim 65 , wherein the oils chosen comprise: about 10% by weight DEET, about 18% by weight d-limonene, about 18% by weight thyme oil, about 20% by weight geraniol, about 20% by weight α-pinene, and about 18% by weight p-cymene.  
     
     
         67 . A composition having insect control activity, the composition comprising at least two plant essential oils, wherein at least one of the two plant essential oils is selected from the group consisting of: t-anthole, black seed oil, camphene, carvacrol, d-carvone, l-carvone, 1,8-cineole, p-cymene, diethyl phthalate, eugenol, geraniol, isopropyl citrate, lemon grass oil, lilac flower oil (LFO), lime oil, d-limonene, linalyl anthranilate, linalool, lindenol, methyl citrate, methyl di-hydrojasmonate, myrcene, perillyl alcohol, phenyl acetaldehyde, α-pinene, β-pinene, piperonal, piperonyl, piperonyl acetate, piperonyl alcohol, piperonyl amine, pulegone, quinine, sabinine, α-terpinene, terpinene 900, α-terpineol, gamma-terpineol, 2-tert-butyl-p-quinone, α-thujone, thyme oil, and thymol, and wherein the composition binds to at least one receptor selected from the group consisting of a tyramine receptor, an Or83b olfactory receptor, and an Or43a olfactory receptor, and wherein the at least two plant essential oils provide a synergistic effect on insect control activity.  
     
     
         68 . The composition of  claim 67 , wherein the at least two plant essential oils comprise lilac flower oil and black seed oil.  
     
     
         69 . The composition of  claim 67 , wherein the at least two plant essential oils comprise thyme oil, lilac flower oil, and d-limonene.  
     
     
         70 . The composition of  claim 67 , wherein the at least two plant essential oils comprise thyme oil, pulegone, and geraniol.  
     
     
         71 . The composition of  claim 67 , wherein a weight proportion of the at least one plant essential oil is about 18% to 50% when the at least one plant essential oil is d-limonene; about 2% to 50% when the at least one plant essential oil is thyme oil; about 18% to 70% when the at least one plant essential oil is geraniol, about 40% to 50% when the at least one plant essential oil is lilac flower oil; and about 44% to 75% when the at least one plant essential oil is black seed oil.