Polypeptides having glucoamylase activity and polynucleotides encoding same

Abstract

The present invention relates to polypeptides having glucoamylase activity and isolated polynucleotides encoding said polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides. The invention also relates to the composition comprising a glucoamylase of the invention as well as the use such compositions for starch conversion processes, brewing, including processes for producing fermentation products or syrups.

1 - 91 . (canceled)  
     
     
         92 . An isolated polypeptide having glucoamylase activity, selected from the group consisting of: 
 (a) a polypeptide having an amino acid sequence which has at least 90% sequence identity with amino acids 1 to 556 of SEQ ID NO: 2; and    (b) a polypeptide having an amino acid sequence which has at least 90% sequence identity with amino acids 1 to 561 of SEQ ID NO: 37.    
     
     
         93 . A fusion polypeptide comprising the polypeptide of  claim 92  and a second polypeptide.  
     
     
         94 . A composition comprising the polypeptide of  claim 92  and an alpha-amylase.  
     
     
         95 . The composition of  claim 94 , wherein the alpha-amylase is a fungal alpha-amylase.  
     
     
         96 . The composition of  claim 94 , wherein the alpha-amylase is obtained from  Aspergillus, Meriplus , or  Rhizomucor.    
     
     
         97 . The composition of  claim 94 , wherein the alpha-amylase is obtained from  Aspergillus awamori, Aspergillus kawachii, Aspergillus niger, Aspergillus oryzae, Meripilus giganteus , or  Rhizomucor pusillus.    
     
     
         98 . The composition of  claim 94 , wherein the alpha-amylase comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 28, SEQ ID NO: 29, and SEQ ID NO: 30.  
     
     
         99 . A process for producing a fermentation product from starch-containing material comprising the steps of: 
 (a) liquefying the starch-containing material in the presence of an alpha-amylase;    (b) saccharifying the liquefied material obtained in step (a) using a polypeptide of  claim 92;  and    (c) fermenting the saccharified material using a fermenting organism.    
     
     
         100 . A process for producing a fermentation product from starch-containing material comprising: 
 (a) saccharifying starch-containing material with a polypeptide of  claim 92  at a temperature below the initial gelatinization temperature of said starch-containing material, and    (b) fermenting using a fermenting organism.    
     
     
         101 . The process of  claim 100 , wherein the polypeptide has an amino acid sequence which has at least 95% sequence identity with amino acids 1 to 556 of SEQ ID NO: 2 or amino acids 1 to 561 of SEQ ID NO: 37 or is a fragment of the sequence of amino acids 1 to 556 of SEQ ID NO: 2 or of amino acids 1 to 561 of SEQ ID NO: 37 which has glucoamylase activity.  
     
     
         102 . The process of  claim 100 , which comprises the sequence of amino acids 1 to 556 of SEQ ID NO: 2 or amino acids 1 to 561 of SEQ ID NO: 37.  
     
     
         103 . The process of  claim 100 , which is a fragment of the sequence of amino acids 1 to 556 of SEQ ID NO: 2 or a fragment of the sequence of amino acids 1 to 561 of SEQ ID NO: 37 which has glucoamylase activity.  
     
     
         104 . An isolated polypeptide having glucoamylase activity, selected from the group consisting of: 
 (a) a polypeptide comprising a catalytic domain having an amino acid sequence which has at least 90% sequence identity with amino acids 1 to 455 of SEQ ID NO: 2; and    (b) a polypeptide comprising a catalytic domain having an amino acid sequence which has at least 90% sequence identity with amino acids 1 to 460 of SEQ ID NO: 37.    
     
     
         105 . The isolated polypeptide of  claim 104 , which comprises a foreign binding domain.  
     
     
         106 . A fusion polypeptide comprising the polypeptide of  claim 104  and a second polypeptide.  
     
     
         107 . A composition comprising a polypeptide of  claim 104  and an alpha-amylase.  
     
     
         108 . A process for producing a fermentation product from starch-containing material comprising the steps of: 
 (a) liquefying the starch-containing material in the presence of an alpha-amylase;    (b) saccharifying the liquefied material obtained in step (a) using a polypeptide of  claim 104;  and    (c) fermenting the saccharified material using a fermenting organism.    
     
     
         109 . A process for producing a fermentation product from starch-containing material comprising: 
 (a) saccharifying starch-containing material with a polypeptide of  claim 104  at a temperature below the initial gelatinization temperature of said starch-containing material, and    (b) fermenting using a fermenting organism.    
     
     
         110 . An isolated polypeptide having carbohydrate binding activity, selected from the group consisting of: 
 (a) a polypeptide comprising a binding domain having an amino acid sequence which has at least 90% sequence identity with amino acids 466 to 556 of SEQ ID NO: 2; and    (b) a polypeptide comprising a binding domain having an amino acid sequence which has at least 90% sequence identity with amino acids 471 to 561 of SEQ ID NO: 37.    
     
     
         111 . The polypeptide of  claim 110 , which further comprises a catalytic domain obtained from a glucoamylase polypeptide.