Vaccines

Abstract

The present invention relates to adjuvant compositions which are suitable to be used in vaccines. In particular, the adjuvant compositions of the present invention comprises a saponin and an immunostimulatory oligonucleotide, optionally with a carrier. Also provided by the present invention are vaccines comprising the adjuvants of the present invention and an antigen. Further provided are methods of manufacture of the adjuvants and vaccines of the present invention and their use as medicaments. Methods of treating an individual susceptible to or suffering from a disease by the administration of the vaccines of the present invention are also provided.

1 .- 8 . (canceled)  
     
     
         9 . A vaccine composition comprising an adjuvant composition comprising a saponin and an immunostimulatory oligonucleotide and further comprising an antigen.  
     
     
         10 .- 29 . (canceled)  
     
     
         30 . A vaccine composition comprising: 
 RTS,S and    an adjuvant composition comprising a saponin and an immunostimulatory oligonucleotide containing an unmethylated CpG motif.    
     
     
         31 . A vaccine composition according to  claim 30 , wherein the saponin is QS 21.  
     
     
         32 . A vaccine composition according to  claim 30  or  31 , wherein the immunostimulatory oligonucleotide is selected from the group comprising:  
       
         
           
                 
                 
                 
               
                     
                 
                   TCC ATG ACG TTC CTG ACG TT 
                   (SEQ ID NO. 1) 
                     
                 
                   (CpG 1826) 
                 
                     
                 
                   TCT CCC AGC GTG CGC CAT 
                   (SEQ ID NO: 2) 
                 
                   (CpG 1758) 
                 
                     
                 
                   ACC GAT GAC GTC GCC GGT GAC GGC ACC 
                 
                   ACG 
                 
                     
                 
                   TCG TCG TTT TGT CGT TTT GTC GTT 
                   (SEQ ID NO: 3) 
                 
                   (CpG 2006) 
                 
                     
                 
                   TCC ATG ACG TTC CTG ATG CT 
                   (SEQ ID NO: 5) 
                 
                   (CpG 1668) 
                 
                     
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         33 . A vaccine composition according to any one of  claims 30  to  32 , which further comprises 3D-MPL.  
     
     
         34 . A vaccine composition according to any one of  claims 30  to  33 , wherein the adjuvant composition is an oil in water emulsion.  
     
     
         35 . A vaccine composition according to any of  claims 30  to  33 , wherein the adjuvant is a liposomal formulation.  
     
     
         36 . A vaccine composition according to any one of  claims 30  to  35 , wherein the immunostimulatory oligonucleotide is in range 1-1000 μg per dose.  
     
     
         37 . A vaccine composition according to any one of  claims 30  to  36 , wherein the saponin is present in the range 1-100 μg per dose.