Salmonella Selective Enrichment Medium Containing Tetrathionate and Magnesium Salt

Abstract

The invention relates to a salmonella selective enrichment medium containing tetrathionate or a salt thereof and at least one type of magnesium salt and to a method for detecting salmonella in a sample by using said selective enrichment medium.

1 - 8 . (canceled) 
     
     
         9 . A salmonella selective enrichment medium comprising tetrathionate, or a salt thereof, and at least one magnesium salt. 
     
     
         10 . The medium according to  claim 9 , wherein the at least one magnesium salt is present at a concentration between 0.1 g/l and 50 g/l. 
     
     
         11 . The medium according to  claim 9 , wherein the at least one magnesium salt is present at a concentration between 2 g/l and 40 g/l. 
     
     
         12 . The medium according to  claim 9 , wherein the at least one magnesium salt is present at a concentration between 7 g/l and 35 g/l. 
     
     
         13 . The medium according to  claim 9 , wherein the at least one magnesium salt is selected from the group consisting of magnesium carbonate, magnesium chloride, magnesium sulfate, and combinations thereof. 
     
     
         14 . The medium according to  claim 9 , wherein the tetrathionate, or salt thereof, is present at a concentration between 0.1 g/l and 40 g/l. 
     
     
         15 . The medium according to  claim 9 , wherein the tetrathionate, or salt thereof, is present at a concentration between 0.5 g/l and 30 g/l. 
     
     
         16 . The medium according to  claim 9 , wherein the tetrathionate, or salt thereof, is present at a concentration between 1 g/l and 25 g/l. 
     
     
         17 . The medium according to  claim 9 , comprising a tetrathionate salt selected from the group consisting of potassium tetrathionate and sodium tetrathionate. 
     
     
         18 . A method for detecting salmonella in a sample, the method comprising:
 incubating the salmonella sample in a salmonella selective enrichment medium comprising tetrathionate, or a salt thereof, and at least one magnesium salt; and   isolating the salmonella.   
     
     
         19 . The method according to  claim 18 , wherein the method further comprises reactivating the salmonella sample prior to the step of incubating in the salmonella selective enrichment medium. 
     
     
         20 . The method according to  claim 19 , wherein the reactivating step comprises incubating the sample in a reactivation medium. 
     
     
         21 . The method according to  claim 18 , wherein the isolation step comprises an analysis procedure selected from the group consisting of enzymatic immunological analyses, nucleic acid probe hybridization analyses, pure culture analyses, analyses using biochemical indicators, and immunoimmobilization analyses. 
     
     
         22 . The method according to  claim 18 , wherein the salmonella selective enrichment medium comprises at least one magnesium salt at a concentration between 0.1 g/l and 50 g/l. 
     
     
         23 . The method according to  claim 18 , wherein the salmonella selective enrichment medium comprises at least one magnesium salt at a concentration between 2 g/l and 40 g/l. 
     
     
         24 . The method according to  claim 18 , wherein the salmonella selective enrichment medium comprises at least one magnesium salt at a concentration between 7 g/l and 35 g/l. 
     
     
         25 . The method according to  claim 18 , wherein the magnesium salt is selected from the group consisting of magnesium carbonate, magnesium chloride, magnesium sulfate, and combinations thereof. 
     
     
         26 . The method according to  claim 18 , wherein the salmonella selective enrichment medium comprises tetrathionate, or a salt thereof, at a concentration between 0.1 g/l and 40 g/l. 
     
     
         27 . The method according to  claim 18 , wherein the salmonella selective enrichment medium comprises tetrathionate, or salt thereof, at a concentration between 0.5 g/l and 30 g/l. 
     
     
         28 . The method according to  claim 18 , wherein the salmonella selective enrichment medium comprises tetrathionate, or salt thereof, at a concentration between 1 g/l and 25 g/l.