Recombinant Parainfluenza Virus Expression Systems And Vaccines Comprising Heterologous Antigens Derived From Respiratory Syncytial Virus

Abstract

The present invention relates to recombinant bovine parainfluenza virus (bPIV) cDNA or RNA which may be used to express heterologous gene products in appropriate host cell systems and/or to rescue negative strand RNA recombinant viruses that express, package, and/or present the heterologous gene product. In particular, the heterologous gene products include gene product of another species of PIV or from another negative strand RNA virus, including but not limited to, influenza virus, respiratory syncytial virus, human metapneumovirus and avian pneumovirus. The chimeric viruses and expression products may advantageously be used in vaccine formulations including vaccines against a broad range of pathogens and antigens.

1 - 48 . (canceled)  
     
     
         49 . A recombinant parainfluenza virus (PIV) comprising: 
 a recombinant bovine PIV type 3 (bPIV3) genome, wherein the bPIV3 genome comprises: 
 bPIV3 nucleotide sequences encoding nucleoprotein (N), phosphoprotein (P), matrix (M), and large protein (L) proteins;  
 human PIV type 3 (hPIV3) nucleotide sequences encoding fusion (F) and hemagglutinin-neuraminidase glycoprotein (HN) proteins, wherein the nucleotide sequences encoding the hPIV3 F and HN proteins substitute for nucleotide sequences encoding bPIV3 F and HN proteins; and  
 a nucleic acid sequence encoding a respiratory syncytial virus (RSV) F protein, wherein the nucleic acid sequence encoding the RSV F protein is inserted at nucleotide position 104 or 1774 of the bPIV3 genome.  
   
     
     
         50 . The recombinant PIV of  claim 49  wherein the nucleic acid sequence encoding the RSV F protein is inserted at nucleotide position 1774 of the bPIV3 genome.  
     
     
         51 . The recombinant PIV of  claim 49  wherein the hPIV3 is hPIV3/Tex/12084/1983.  
     
     
         52 . The recombinant PIV of  claim 50  wherein the hPIV is hPIV3/Tex/12084/1983.  
     
     
         53 . The recombinant PIV of  claim 49  wherein the bPIV3 is Kansas-strain bPIV3.  
     
     
         54 . The recombinant PIV of  claim 50  wherein the bPIV3 is Kansas strain bPIV3.  
     
     
         55 . The recombinant PIV of  claim 52  wherein the bPIV3 is Kansas-strain bPIV3.  
     
     
         56 . The recombinant PIV of  claim 49  wherein the RSV F protein comprises the amino acid sequence shown in SEQ ID NO:7.  
     
     
         57 . The recombinant PIV of  claim 50  wherein the RSV F protein comprises the amino acid sequence shown in SEQ ID NO:7.  
     
     
         58 . The recombinant PIV of  claim 52  wherein the RSV F protein comprises the amino acid sequence shown in SEQ ID NO:7.  
     
     
         59 . The recombinant PIV of  claim 54  wherein the RSV F protein comprises the amino acid sequence shown in SEQ ID NO:7.  
     
     
         60 . The recombinant PIV of  claim 55  wherein the RSV F protein comprises the amino acid sequence shown in SEQ ID NO:7.  
     
     
         61 . The recombinant PIV of  claim 49  wherein the nucleic acid sequence encoding the RSV F protein is inserted at nucleotide position 104 of the bPIV3 genome.  
     
     
         62 . The recombinant PIV of  claim 61  wherein the hPIV is hPIV3/Tex/12084/1983.  
     
     
         63 . The recombinant PIV of  claim 61  wherein the bPIV3 is Kansas strain bPIV3.  
     
     
         64 . The recombinant PIV of  claim 62  wherein the bPIV3 is Kansas-strain bPIV3.  
     
     
         65 . The recombinant PIV of  claim 61  wherein the RSV F protein comprises the amino acid sequence shown in SEQ ID NO:7.  
     
     
         66 . The recombinant PIV of  claim 62  wherein the RSV F protein comprises the amino acid sequence shown in SEQ ID NO:7.  
     
     
         67 . The recombinant PIV of  claim 63  wherein the RSV F protein comprises the amino acid sequence shown in SEQ ID NO:7.  
     
     
         68 . The recombinant PIV of  claim 64  wherein the RSV F protein comprises the amino acid sequence shown in SEQ ID NO:7.