Antibodies That Specifically Bind PMS2

Abstract

Antibodies against PMS2 and PMS2-134 and cells that produce the anti-PMS2 and anti-PMS2-134 antibodies are provided. The antibodies of the invention may be used in methods for detecting a PMS2 protein, including a truncated PMS2, and in methods for detecting an abnormal condition in a patient.

1 . A method for detecting a truncated form of a PMS2 protein comprising preparing a cell lysate from a test cell, exposing said lysate to an antibody that specifically binds an epitope of SEQ ID NO: 1, and detecting said antibody. 
     
     
         2 . The method of  claim 1  wherein said epitope is located between amino acids 55 and 81 of SEQ ID NO: 1. 
     
     
         3 . The method of  claim 1  wherein said epitope is located between amino acids 81 and 133 of SEQ ID NO: 1. 
     
     
         4 . A method for detecting an abnormal condition in a patient expressing a truncated PMS2, said method comprising contacting a test cell lysate from said patient with an antibody that specifically binds an epitope of SEQ ID NO: 1 and detecting the presence or absence of a truncated form of PMS2, wherein the presence of said truncated PMS2 is indicative of an abnormal condition. 
     
     
         5 . The method of  claim 4  wherein said epitope is located between amino acids 55 and 81 of SEQ ID NO: 1. 
     
     
         6 . The method of  claim 4  wherein said epitope is located between amino acids 81 and 133 of SEQ ID NO: 1. 
     
     
         7 . The method of  claim 4  wherein said abnormal condition is cancer.