Ribonucleic acid interference molecules

Abstract

Ribonucleic acid interference molecules are provided. For example, in one aspect of the invention, at least one nucleic acid molecule comprising at least one of one or more precursor sequences having SEQ ID NO: 1 through SEQ ID NO: 103,948 and one or more mature sequences having SEQ ID NO: 1 through SEQ ID NO: 126,499 is provided. One or more of the at least one of one or more precursor sequences and one or more mature sequences may be computationally predicted, e.g., from publicly available genomes, using a pattern discovery method. In another aspect of the invention, a method for regulating gene expression comprises the following step. At least one nucleic acid molecule comprising at least one of one or more precursor sequences having SEQ ID NO: 1 through SEQ ID NO: 103,948, each one of the precursor sequences containing one or more mature sequences having SEQ ID NO: 1 through SEQ ID NO: 126,499, is used to regulate the expression of one or more genes, e.g., by inducing post-transcriptional silencing of the one or more genes.

1 . At least one nucleic acid molecule, comprising:
 at least one of one or more precursor sequences having SEQ ID NO: 1 through SEQ ID NO: 103,948 and one or more mature sequences having SEQ ID NO: 103,949 through SEQ ID NO: 230,447.   
     
     
         2 . The at least one nucleic acid molecule of  claim 1 , wherein one or more of the at least one of one or more precursor sequences and one or more mature sequences have been computationally predicted using a pattern discovery method. 
     
     
         3 . The at least one nucleic acid molecule of  claim 1 , wherein one or more of the at least one of one or more precursor sequences and one or more mature sequences regulate gene expression in one or more genes by inducing post-transcriptional silencing of the one or more genes. 
     
     
         4 . The at least one nucleic acid molecule of  claim 1 , wherein one or more of the sequences encode ribonucleic acid sequences. 
     
     
         5 . The at least one nucleic acid molecule of  claim 1 , wherein one or more of the sequences encode interfering ribonucleic acid sequences. 
     
     
         6 . The at least one nucleic acid molecule of  claim 1 , wherein the precursor sequences having SEQ ID NO: 1 through SEQ ID NO: 57,431 are derived from a genomic sequence corresponding to  H. sapiens.    
     
     
         7 . The at least one nucleic acid molecule of  claim 1 , wherein the precursor sequences having SEQ ID NO: 57,432 through SEQ ID NO: 101,967 are derived from a genomic sequence corresponding to  M. musculus.    
     
     
         8 . The at least one nucleic acid molecule of  claim 1 , wherein the precursor sequences having SEQ ID NO: 101,968 through SEQ ID NO: 103,203 are derived from a genomic sequence corresponding to  D. melanogaster.    
     
     
         9 . The at least one nucleic acid molecule of  claim 1 , wherein the precursor sequences having SEQ ID NO: 103,204 through SEQ ID NO: 103,948 are derived from a genomic sequence corresponding to  C. elegans.    
     
     
         10 . The at least one nucleic acid molecule of  claim 1 , wherein the mature sequences having SEQ ID NO: 103,949 through SEQ ID NO: 173,336 are derived from a genomic sequence corresponding to  H. sapiens.    
     
     
         11 . The at least one nucleic acid molecule of  claim 1 , wherein the mature sequences having SEQ ID NO: 173,337 through SEQ ID NO: 228,005 are derived from a genomic sequence corresponding to  M. musculus.    
     
     
         12 . The at least one nucleic acid molecule of  claim 1 , wherein the mature sequences having SEQ ID NO: 228.006 through SEQ ID NO: 229,484 are derived from a genomic sequence corresponding to  D. melanogaster.    
     
     
         13 . The at least one nucleic acid molecule of  claim 1 , wherein the mature sequences having SEQ ID NO: 229,485 through SEQ ID NO: 230,447 are derived from a genomic sequence corresponding to  C. elegans.    
     
     
         14 . A method for regulating gene expression, the method comprising the step of:
 using at least one nucleic acid molecule, comprising at least one of one or more precursor sequences having SEQ ID NO: 1 through SEQ ID NO: 103,948 or one or more mature sequences having SEQ ID NO: 103,949 through SEQ ID NO: 230,447 to regulate the expression of one or more genes.   
     
     
         15 . The method of  claim 14 , further comprising the step of inserting the at least one nucleic acid molecule into an environment where the at least one nucleic acid molecule can be produced biochemically. 
     
     
         16 . The method of  claim 14 , further comprising the step of inserting the at least one nucleic acid molecule in to an environment where the at least one nucleic acid molecule can be produced biochemically, giving rise to one or more interfering ribonucleic acids which affect one or more target sequences. 
     
     
         17 . The method of  claim 14 , wherein one or more of the sequences are synthetically removed from the genome that contains them naturally. 
     
     
         18 . The method of  claim 14 , wherein one or more of the sequences are synthetically introduced in a genome that does not contain them naturally. 
     
     
         19 . The method of  claim 14 , wherein one or more target sequences are encoded by a same genome as the one or more sequences. 
     
     
         20 . The method of  claim 14 , wherein one or more target sequences are encoded by a different genome from the one or more sequences. 
     
     
         21 . The method of  claim 14 , wherein one or more sequences are transcribed, giving rise to one or more interfering ribonucleic acids which induce post-transcriptional repression of one or more target sequences. 
     
     
         22 . The method of  claim 14 , wherein the one or more sequences are transcribed, giving rise to one or more interfering ribonucleic acids which induce gene silencing of one or more target sequences. 
     
     
         23 . The method of  claim 14 , wherein one or more target sequences are naturally occurring. 
     
     
         24 . The method of  claim 14 , wherein one or more target sequences are synthetically constructed. 
     
     
         25 . The method of  claim 14 , wherein one or more of the sequences are synthetically constructed. 
     
     
         26 . At least one nucleic acid molecule, comprising:
 at least a portion of a precursor sequence having one of SEQ ID NO: 1 through SEQ ID NO: 103,948, wherein the portion comprises an amount of the sequence that does not significantly alter a behavior of the complete precursor sequence.   
     
     
         27 . At least one nucleic acid molecule, comprising: at least a portion of a mature sequence having one of SEQ ID NO: 103,949 through SEQ ID NO: 230,447, wherein the portion comprises an amount of the sequence that does not significantly alter a behavior of the complete mature sequence.