Method for detecting or measuring HBV

Abstract

A Method for detecting hepatitis B virus as well as a kit and a reagent therefor, comprising using a phosphate buffer that specifically binds to hepatitis B virus, for example a monoclonal antibody that specifically binds to a specific site of core-related protein of hepatitis B virus.

1 . A method for detecting or quantitating HBV core antigen and HBV e antigen, comprising:
 (1) denaturing of particles in a test sample suspected of containing hepatitis B virus to form a test sample;   (2) reacting the test sample suspected of containing hepatitis B virus with an antibody that recognizes and binds to amino acid Nos. 31-49, Nos. 168-176, or a structural epitope present in the region of Nos. 1-81, of the hepatitis B virus (HBV) core polypeptide at 1.67% sodium dodecyl sulfate; and   (3) detecting the captured hepatitis B virus core antigen and hepatitis B e antigen by a second antibody to hepatitis B core antigen and hepatitis B e antigen.   
     
     
         2 . A kit for detecting or quantitating the captured hepatitis B virus core antigen and hepatitis B e antigen, comprising an antibody that recognizes and binds to amino acid Nos. 31-49, Nos. 168-176, or a structural epitope present in the region of Nos. 1-81, of the hepatitis B virus (HBV) core polypeptide at 1.67% sodium dodecyl sulfate.