Coating device and method for coating medical device with bioactive peptide

Abstract

A method for coating a medical device with a bioactive peptide comprising: providing a medical device to be coated with a bioactive peptide to a cylinder; attaching a syringe containing a bioactive peptide coating solution; introducing into the cylinder the bioactive peptide coating solution when the first valve of the connector and the second valve of the connector are open; incubating the medical device within the cylinder with the bioactive peptide coating solution; removing the bioactive peptide coating solution from the cylinder after the medical device is coated with the bioactive peptide.

1 . A method for coating a medical device with a bioactive peptide comprising:
 providing a medical device to be coated with a bioactive peptide to a cylinder having a plunger that inserts into a first end of the cylinder and fits snuggly against the sides of the cylinder and at the other end of the cylinder is located a connector having a first valve that is attached to the second end of the cylinder for creating a closed cylinder when the first valve is closed;   attaching a syringe containing a bioactive peptide coating solution to a second valve of the connector;   introducing into the cylinder the bioactive peptide coating solution when the first valve of the connector and the second valve of the connector are open;   incubating the medical device within the cylinder with the bioactive peptide coating solution;   removing the bioactive peptide coating solution from the cylinder after the medical device is coated with the bioactive peptide.   
     
     
         2 . The method of  claim 1  wherein the step of incubating further comprises placing the cylinder in a substantially horizontal position to increase the solution to granule contact. 
     
     
         3 . The method of  claim 1  wherein the cylinder is a first syringe. 
     
     
         4 . The method of  claim 1  wherein the medical device is osteoconductive material. 
     
     
         5 . The method of  claim 1  wherein the cylinder is in a substantially vertical position during the introducing step. 
     
     
         6 . The method of  claim 1  wherein the plunger of the cylinder is pushed to discharge the bioactive peptide coating solution from the cylinder. 
     
     
         7 . The method of  claim 1  wherein the bioactive peptide coating solution comprises a synthetic growth factor analogue comprising a non-growth factor heparin binding region, a linker and a sequence that binds specifically to a cell surface receptor. 
     
     
         8 . A system for coating a medical device with a bioactive peptide comprising:
 a cylinder for housing a medical device to be coated with a bioactive peptide coating solution wherein the cylinder comprises a plunger that inserts into a first end of the cylinder and fits snuggly against the sides of the cylinder and at the other end of the cylinder is located a connector having a first valve that is attached to the second end of the cylinder for creating a closed cylinder when the first valve is closed;   a syringe that is attached to a second valve of the connector wherein the syringe contains a bioactive peptide coating solution that is introduced into the cylinder via the connector; and   a connector having a first valve attached to the cylinder and a second valve attached to the syringe.   
     
     
         9 . A kit for use in coating a medical device with a bioactive peptide comprising:
 a bioactive peptide;   a first syringe;   a second syringe and   a connector having a first valve and a second valve.   
     
     
         10 . The kit of  claim 9  wherein the bioactive peptide comprises a synthetic growth factor analogue comprising a non-growth factor heparin binding region, a linker and a sequence that binds specifically to a cell surface receptor.