Lipid bilayers on nanotextured solid surfaces

Abstract

The present disclosure provides various novel suspended planar lipid bilayer assemblies made from bicellar mixtures containing long and short chain phospholipids and methods of making the same. Such bilayer assemblies may additionally incorporate biomolecules such as proteins, polypeptides, biological complexes, transmembrane proteins and other membrane-associated compounds. The present disclosure further provides uses for such lipid bilayer assemblies including proteomics, membrane study, biosensing for medical diagnosis and environmental monitoring, chemical and biological warfare agent sequestration, actuator development, and bio-fuel cell development.

1 . A suspended lipid bilayer comprising a mixture of short-chain and long-chain phospholipids on a solid support, wherein the short-chain and long-chain phospholipids are homogenously distributed throughout the lipid bilayer and the support is such that fluid may flow under the suspended portion of the lipid bilayer. 
     
     
         2 . The suspended lipid bilayer of  claim 1 , wherein the lipids are fluid within the suspended portion of the lipid bilayer. 
     
     
         3 . The suspended lipid bilayer of  claim 1 , wherein the solid support is silica, mica, glass, aluminum, oxidized silicon, semiconductor chips, biochips, silicon wafer, silane-silicon, self assembled monolayer-gold, SnO 2 , polymer coated substrates, gold, gold-SAM, or porous Alumina. 
     
     
         4 . The suspended lipid bilayer of  claim 1 , wherein the solid support is oxidized silicon. 
     
     
         5 . The suspended lipid bilayer of  claim 1 , wherein the solid support has a nanotextured surface. 
     
     
         6 . The suspended lipid bilayer of  claim 5 , wherein the nanotextured surface comprises parallel troughs. 
     
     
         7 . The suspended lipid bilayer of  claim 6 , wherein the parallel troughs have a width of about 20 nm to about 500 nm. 
     
     
         8 . The suspended lipid bilayer of  claim 1  further comprising a biomolecule. 
     
     
         9 . The suspended lipid bilayer of  claim 1 , wherein the suspended lipid bilayer comprises two or more phospholipids selected from a group consisting of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-diheptanoyl-sn-glycero-3-phosphocholine (DHPC), 1,2-dihexanoyl-sn-glycero-3-phosphoethanolamine (DHPE), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-DiODodecyl-sn-Glycero-3-Phosphocholine (DIODPC), 3-(ChlorAmidoPropyl)-dimethylammonio2-Hydroxy-1-Propane Sulfonate (CHAPSO), dimyristoyl phophatidylserine (DMPS), dimyristoyl phosphatidylglycerol, dilauryl phosphatidycholine (DLPC), 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE; 14:0); 1,2-dimyristoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (Sodium Salt) (DMPG, 14:0), 1-myristoyl-2-hydroxy-sn-glycero-3-phosphocholine (14:0 Lyso PC), 1-oleoyl-2-hydroxy-sn-glycero-3-phosphoethanolamine (18:1 Lyso PE), L-phosphatidylcholine (Egg, Soy), phosphatidylcholine (NBD), 1,1′,2,2′-tetramyristoyl cardiolipin (Ammonium Salt) (14:0), lipids with head groups phosphatidyl serine and phosphatidylinositol, poly(ethylene glycol)-lipid conjugates, fluoroscent lipids-phosphatidylcholine (NBD), 1,2-didecanoyl-sn-glycero-3-phosphocholine (DDPC), 1,2-dierucoyl-sn-glycero-3-phosphate (sodium salt) (DEPA-NA), 1,2-erucoyl-sn-glycero-3-phosphocholine (DEPC), 1,2-dierucoyl-sn-glycero-3-phosphoethanolamine (DEPE), 1,2-linoleoyl-sn-glycero-3-phosphocholine (DLOPC), 1,2-dilauroyl-sn-glycero-3-phosphate (sodium salt) (DLPA-NA), 1,2-dilauroyl-sn-glycero-3-phosphoethanolamine (DLPE), 1,2-dilauroyl-sn-glycero-3-phosphoserine (sodium salt) (DLPS-NA), 1,2-dimyristoyl-sn-glycero-3-phosphate (sodium salt) (DMPA-NA), 1,2-dimyristoyl-sn-glycero-3-phosphoserine (sodium salt) (DMPS-NA), 1,2-dioleoyl-sn-glycero-3-phosphate (sodium salt) (DOPA-NA), 1,2-oleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-dioleoyl-sn-glycero-3-phosphoserine (sodium salt) (DOPS-NA), 1,2-dipalmitoyl-sn-glycero-3-phosphate (sodium salt) (DPPA-NA), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE), 1,2-dipalmitoyl-sn-glycero-3-phosphoserine (sodium salt) (DPPS-NA), 1,2-distearoyl-sn-glycero-3-phosphate (sodium salt) (DSPA-NA), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-diostearpyl-sn-glycero-3-phosphoethanolamine (DSPE), 1,2-distearoyl-sn-glycero-3-phosphoserine (sodium salt) DSPS-NA, 1-myristoyl, 2-stearoyl-sn-glycero 3-phosphocholine (MSPC), 1-palmitoyl, 2-myristoyl-sn-glycero 3-phosphocholine (PMPC), 1-palmitoyl, 2-oleoyl-sn-glycero 3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl, 2-stearoyl-sn-glycero 3-phosphocholine (PSPC), 1-stearoyl, 2-myristoyl-sn-glycero 3-phosphocholine (SMPC), 1-stearoyl, 2-palmitoyl-sn-glycero 3-phosphocholine (SOPC), or 1-stearoyl, 2-palmitoyl-sn-glycero 3-phosphocholine (SPPC). 
     
     
         10 . The suspended lipid bilayer of  claim 1 , wherein the long chain phospholipid is DPPC. 
     
     
         11 . The suspended lipid bilayer of  claim 1 , wherein the short chain phospholipid is DHPC. 
     
     
         12 . The suspended lipid bilayer of  claim 1 , further comprising a layer of aqueous solution between the supported lipid bilayer and the solid support. 
     
     
         13 . The suspended lipid bilayer of  claim 1 , wherein the short chain and long chain phospholipids are in a q ratio of about 2.8. 
     
     
         14 . A method for making a supported lipid bilayer on a solid support comprising:
 (a) mixing an aqueous solution of short-chain phospholipids with long chain phospholipids;   (b) placing the solid support on a drop of the lipid suspension; and   (c) rinsing the solid support with an aqueous solution.   
     
     
         15 . The method of  claim 14 , wherein the aqueous solution further comprises a biomolecule. 
     
     
         16 . The method of  claim 14 , wherein the solid support is silica, mica, glass, aluminum, oxidized silicon, semiconductor chips, biochips, silicon wafer, silane-silicon, self assembled monolayer-gold, SnO 2 , polymer coated substrates, gold, gold-SAM, or porous Alumina. 
     
     
         17 . The method of  claim 14 , wherein the solid support has a nanotextured surface. 
     
     
         18 . The method of  claim 17 , wherein the nanotextured surface comprises a series of parallel troughs. 
     
     
         19 . The method of  claim 18 , wherein the parallel troughs have a width of about 20 nm to about 500 nm. 
     
     
         20 . The method of  claim 14 , wherein the short and long chain phospholipids are in a q ratio of about 2.8.