One-tube nested PCR for detecting Mycobacterium tuberculosis

Abstract

The present invention relates to a kit and method for detecting M. tuberculosis of suspected patient. The present invention also relates to primers and probe used to detect M. tuberculosis by performing one tube nested PCR.

1 - 20 . (canceled) 
     
     
         21 . A nucleic acid comprising a nucleotide sequence for detecting the presence or absence of  M. tuberculosis , said nucleotide sequence selected from the group consisting of: 
       
         
           
                 
                 
                 
               
                   5′-CACGTAGGCG AACCCTGCCC AGGTC-3′; 
                   SEQ ID NO: 2 
                     
                 
                     
                 
                   5′-GCGTAGGCGT CGGTCACAAA-3′; 
                   SEQ ID NO: 3 
                 
                     
                 
                   5′-GTGAGGGCAT CGAGGTGGCC AGATG-3′; 
                   SEQ ID NO: 4 
                 
                     
                 
                   5′-GACACATAGG TGAGGTCTGC-3′; 
                   SEQ ID NO: 6 
                 
                     
                 
                   5′-GCCACGTAGG CGAACCCTG-3′; 
                   SEQ ID NO: 7 
                 
                     
                 
                   5′-ACGGCTGA TGACCAAACT-3′; 
                   SEQ ID NO: 8 
                 
                     
                 
                   5′-AGATGCACCG TCGAACGG-3′; 
                   SEQ ID NO: 9 
                 
                   and 
                 
                     
                 
                   TTTTTTTTTTACCTAACCGGCTGTGGGTAGCAGA. 
                   SEQ ID NO: 10.