Insulin Derivatives

Abstract

The present invention is related to insulin derivatives having a side chain attached either to the α-amino group of the N-terminal amino acid residue of B chain or to an ε-amino group of a Lys residue present in the B chain of the parent insulin molecule via an amide bond which side chain comprises one or more residues of ethyleneglycol, propyleneglycol and/or butyleneglycol containing independently at each termini a group selected from —NH 2 and —COOH; a fatty diacid moiety with 4 to 22 carbon atoms, at least one free carboxylic acid group or a group which is negatively charged at neutral pH; and possible linkers which link the individual components in the side chain together via amide or ether bonds, said linkers optionally comprising a free carboxylic acid group.

1 . An insulin derivatives having a side chain attached either to the α-amino group of the N-terminal amino acid residue of B chain or to an ε-amino group of a Lys residue present in the B chain of the parent insulin molecule via an amide bond which side chain comprises one or more residues of ethyleneglycol, propyleneglycol and/or butyleneglycol containing independently at each termini a group selected from —NH 2  and —COOH; a fatty diacid moiety with from 4 to 22 carbon atoms, at least one free carboxylic acid group or a group which is negatively charged at neutral pH; and possible linkers which link the individual components in the side chain together via amide or ether bonds, said linkers optionally comprising a free carboxylic acid group. 
     
     
         2 . The insulin derivative according to  claim 1 , wherein PEG or PPG or PBG group has from 2 to 20; from 2 to 10 or from 2 to 5 residues of ethyleneglycol, propyleneglycol or butyleneglycol. 
     
     
         3 . The insulin derivative according to  claim 1 , wherein the sidechain comprises a single residue of ethyleneglycol. 
     
     
         4 . The insulin derivative according to  claim 1 , wherein the sidechain comprises single residues of ethyleneglycol, propyleneglycol and butyleneglycol alone or in combination. 
     
     
         5 . The insulin derivative according to  claim 4 , wherein the sidechain comprises one residue of propyleneglycol and one residue of butyleneglycol. 
     
     
         6 . The insulin derivative according to  claim 1 , wherein the fatty diacid comprises from 4 to 22 carbon atoms in the carbon chain. 
     
     
         7 . The insulin derivative according to  claim 6 , wherein the fatty diacid comprises from 6 to 22, from 8 to 20, from 8 to 18, from 4 to 18, from 6 to 18, from 8 to 16, from 8 to 22, from 8 to 17 or from 8 to 15 carbon atoms in the carbon chain. 
     
     
         8 . The insulin derivative according to  claim 1 , wherein the linker is an amino acid residue, a peptide chain of 2-4 amino acid residues or has the motif α-Asp, β-Asp, α-Glu, γ-Glu, α-hGlu, δ-hGlu, —N(CH 2 COOH)CH 2 CO—, —N(CH 2 CH 2 COOH)CH 2 CH 2 CO—, —N(CH 2 COOH)CH 2 CH 2 CO— or —N(CH 2 CH 2 COOH)CH 2 CO— 
     
     
         9 . The insulin derivative according to  claim 1 , wherein the Lys residue in the B chain of the parent insulin in either position B3, B29 or in one of positions B23-30. 
     
     
         10 . The insulin derivative according to  claim 1  having the formula 
       
         
           
           
               
               
           
         
       
       wherein Ins is the parent insulin moiety which via the α-amino group of the N-terminal amino acid residue of the B chain or an εamino group of a Lys residue present in the B chain of the insulin moiety is bound to the CO— group in the side chain via an amide bond;
 each n is independently 0, 1, 2, 3, 4, 5 or 6; 
 Q 1 , Q 2 , Q 3 , and Q 4  independently of each other can be selected from: (CH 2 CH 2 O) s —; (CH 2 CH 2 CH 2 O) s —; (CH 2 CH 2 CH 2 CH 2 O) s —; (CH 2 CH 2 OCH 2 CH 2 CH 2 CH 2 O) s — or (CH 2 CH 2 CH 2 OCH 2 CH 2 CH 2 CH 2 O) s — where s is 1-20: —(CH 2 ) r  where r is an integer from 4 to 22; or a divalent hydrocarbon chain comprising 1, 2 or 3 —CH═CH— groups and a number of —CH 2 — groups sufficient to give a total number of carbon atoms in the chain in the range of 4 to 22; —(CH 2 ) t — or —(CH 2 OCH 2 ) t —, where t is an integer from 1 to 6; —(CR 1 R 2 ) q —, where R 1  and R 2  independently of each other can be H, —COOH, (CH 2 ) 1-6 COOH and R 1  and R 2  can be different at each carbon, and q is 1-6; —((C 3 R 4 ) q1 ) 1 —(NHCO—(CR 3 R 4 ) q1 —NHCO) 1-2 —((CR 3 R 4 ) q1 ) 1  or —((CR 3 R 4 ) q1 ) 1 —(CONH—(CR 3 R 4 ) q1 —CONH) 1-2 —(CR 3 R 4 ) q1 —)-, ((CR 3 R 4 ) q1 ) 1 —(NHCO—(CR 3 R 4 ) q1 —CONH) 1-2 —((CR 3 R 4 ) q1 ) 1  or —((CR 3 R 4 ) q1 ) 1 —(CONH—(CR 3 R 4 ) q1 —NHCO) 1-2 —((CR 3 R 4 ) q1 ) 1  where R 3  and R 4  independently of each other can be H, —COOH, and R 3  and R 4  can be different at each carbon, and q 1  is 1-6-; and a bond; 
 with the proviso that Q 1 -Q 4  are different; 
 X 1 , X 2  and X 3  are independently selected from: O; a bond; 
 
       
         
           
           
               
               
           
         
         where R is hydrogen or —(CH 2 ) p —COOH, —(CH 2 ) p —SO 3 H, —(CH 2 ) p —PO 3 H 2 , —(CH 2 ) p —O—SO 3 H; —(CH 2 ) p —O—PO 3 H 2 ; or —(CH 2 ) p -tetrazol-5-yl, where each p independently of the other p's is an integer in the range of 1 to 6; and 
         Z is selected from: —COOH; —CO-Asp; —CO-Glu; —CO-Gly; —CO-Sar; —CH(COOH) 2 ; —N(CH 2 COOH) 2 ; —SO 3 H; —OSO 3 H; —OPO 3 H 2 ; —PO 3 H 2  and -tetrazol-5-yl and any Zn 2+  complex thereof. 
       
     
     
         11 . The insulin derivative according to  claim 10 , wherein s is in the range of 2-12, 2-4 or 2-3 
     
     
         12 . The insulin derivative according to  claim 10 , wherein s is preferably 1. 
     
     
         13 . The insulin derivative according to  claim 10 , wherein Z is —COOH. 
     
     
         14 . The insulin derivative according to  claim 1 , wherein the parent insulin is a desB30 human insulin analogue. 
     
     
         15 . The insulin derivative according to  claim 1 , wherein the parent insulin is selected from the group consisting of human insulin; desB1 human insulin; desB30 human insulin; GlyA21 human insulin; GlyA21 desB30 human insulin; AspB28 human insulin; porcine insulin; LysB28 ProB29 human insulin; GlyA21 ArgB31 ArgB32 human insulin; and LysB3 GluB29 human insulin or AspB28 desB30 human insulin. 
     
     
         16 . The insulin derivative according to  claim 1  selected from the group consisting of N εB29 -(3-[2-{2-(2-[(ω-carboxy-pentadecanoyl-γ-glutamyl-(2-amino-ethoxy)]-ethoxy)-ethoxy}-ethoxy]-propinoyl) desB30 human insulin, N εB29 -(3-[2-{2-(2-[(ω-carboxy-heptadecanoyl-γ-glutamyl-(2-amino-ethoxy)]-ethoxy)-ethoxy}-ethoxy]-propinoyl) desB30 human insulin, N εB29 -{3-[2-(2-{2-[2-(ω-carboxy-pentadecanoylamino)-ethoxy]-ethoxy}-ethoxy)-ethoxy]-propionyl-γ-glutamyl desB30 human insulin, N εB29 -(ω-[2-(2-{2-[2-(2-carboxy-ethoxy)-ethoxy]-ethoxy}-ethoxy)-ethylcarbamoyl]-heptadecanoyl-α-glutamyl) desB30 human insulin, N εB29 -(ω-[2-(2-{2-[2-(2-carboxy-ethoxy)-ethoxy]-ethoxy}-ethoxy)-ethylcarbamoyl]-heptadecanoyl-γ-glutamyl) desB30 human insulin, N εB29 -3-[2-(2-{2-[2-(ω-carboxy-heptadecanoylamino)-ethoxy]-ethoxy}-ethoxy)-ethoxy]-propionyl-γ-glutamyl desB30 human insulin, N εB29 -(3-(3-{2-[2-(3-[7-carboxyheptanoylamino]propoxy)ethoxy]-ethoxy}propylcarbamoyl)propionyl) desB30 human insulin, N εB29 -(3-(3-{4-[3-(7-Carboxyheptanoylamino)propoxy]butoxy}propylcarbamoyl)-propionyl-γ-glutamyl) desB30 human insulin, N εB29 -(3-(3-{2-[2-(3-[9-Carboxynonanoylamino]propoxy)ethoxy]ethoxy}-propylcarbamoyl)propionyl) desB30 human insulin, N εB29 -(3-(2-{2-[2-(9-carboxynonanoylamino)ethoxy]ethoxy}ethylcarbamoyl)propionyl-γ-glutamyl) desB30 human insulin, N εB29 -(3-(3-{4-[3-(9-Carboxynonanoylamino)propoxy]butoxy}-propylcarbamoyl)propionyl-γ-glutamyl) desB30 human insulin, N εB29 -(2-[3-(2-(2-{2-(7-carboxyheptanoylamino)ethoxy}ethoxy)-ethylcarbamoyl]propionyl-γ-glutamyl) desB30 human insulin, N εB29 -(3-[2-(2-{2-[2-(ω-carboxypentadecanoylamino)ethoxy]ethoxy}-ethoxy)ethoxy]propionyl)) desB30 human insulin, N εB29 -(3-(2-{2-[2-(2-{2-[2-(2-{2-[2-(2-{2-[2-(ω-carboxy-tridecanoylamino)-ethoxy]-ethoxy}-ethoxy)-ethoxy]-ethoxy}-ethoxy)-ethoxy]-ethoxy}-ethoxy)-ethoxy]-ethoxy}-ethoxy)-propionoyl-γ-glutamyl) desB30 human insulin, N εB29 -(3-[2-(2-{2-[2-(ω-Carboxy-tridecanoylamino)-ethoxy]-ethoxy}-ethoxy)-ethoxy]-propionoyl-γ-glutamyl) desB30 human insulin, N εB29 -(3-[2-(2-{2-[2-(2-{2-[2-(ω-carboxy-tridecanoylamino)-ethoxy]-ethoxy}-ethoxy)-ethoxy]-ethoxy}-ethoxy)-ethoxy]-ethoxy}-propionoyl-γ-glutamyl) desB30 human insulin, N εB29 -(3-(2-{2-[2-(ω-Carboxy-pentadecanoylamino)-ethoxy]-ethoxy}-ethylcarbamoyl)-propionyl-γ-glutamyl) desB30 human insulin, N εB29 -(3-(3-{2-[2-(3-[(ω-Carboxypentadecanoylamino]propoxy)ethoxy]-ethoxy}propylcarbamoyl)propionyl-γ-glutamyl) desB30 human insulin, N εB29 -(3-(3-{4-[3-(ω-Carboxyundecanoylamino)propoxy]butoxypropylcarbamoyl)propionyl-γ-glutamyl) desB30 human insulin, N εB29 -(3-(3-{4-[3-(ω-carboxytridecanoylamino)propoxy]-butoxypropylcarbamoyl)propionyl-γ-glutamyl) desB30 human insulin, N εB29 -(3-(2-{2-[2-(ω-Carboxyundecanoylamino)ethoxy]ethoxy}ethylcarbamoyl)propionyl-γ-glutamyl) desB30 human insulin, N εB29 -(3-(2-{2-[2-(ω-carboxytridecanoylamino)ethoxy]ethoxy}-ethylcarbamoyl)propionyl-γ-glutamyl) desB30 human insulin, N εB29 -{3-[2-(2-{2-[2-(ω-carboxy-pentadecanoylamino)ethoxy]ethoxy}ethoxy)ethoxy]propionyl-gamma-γ-D-glutamyl) desB30 human insulin, N εB29 -{3-[2-(2-{2-[2-(7-carboxyheptanoylamino)-ethoxy]ethoxy}ethoxy)ethoxy]propionyl-γ-glutamyl} desB30 human insulin, N εB29 -{3-[2-(2-{2-[2-(9-carboxynonanoylamino)ethoxy]ethoxy}ethoxy)ethoxy]propioniyl-γ-glutamyl} desB30 human insulin, N εB29 -{3-[2-(2-{2-[2-(ω-carboxyundecanoylamino)-ethoxy]ethoxy}ethoxy)ethoxy]propionyl-γ-glutamyl} desB30 human insulin, N εB29 -{3-[2-(2-{2-[2-(ω-carboxytridecanoylamino)ethoxy]ethoxy}ethoxy)ethoxy]propionyl-γ-glutamyl} desB30 human insulin. 
     
     
         17 . (canceled) 
     
     
         18 . (canceled) 
     
     
         19 . (canceled) 
     
     
         20 . A method of treating diabetes in a patient in need of such a treatment, comprising administering to the patient a therapeutically effective amount of an insulin derivative, said insulin derivative having a side chain attached either to the α-amino group of the N-terminal amino acid residue of B chain or to an ε-amino group of a Lys residue present in the B chain of the parent insulin molecule via an amide bond which side chain comprises one or more residues of ethyleneglycol, propyleneglycol and/or butyleneglycol containing independently at each termini a group selected from —NH 2  and —COOH; a fatty diacid moiety with from 4 to 22 carbon atoms at least one free carboxylic acid group or a group which is negatively charged at neutral pH; and possible linkers which link the individual components in the side chain together via amide or ether bonds, said linkers optionally comprising a free carboxylic acid group together with a pharmaceutically acceptable carrier. 
     
     
         21 . A method of treating diabetes in a patient in need of such a treatment, comprising administering to the patient a therapeutically effective amount of an insulin derivative, said insulin derivative having a side chain attached either to the α-amino group of the N-terminal amino acid residue of B chain or to an ε-amino group of a Lys residue present in the B chain of the parent insulin molecule via an amide bond which side chain comprises one or more residues of ethyleneglycol, propyleneglycol and/or butyleneglycol containing independently at each termini a group selected from —NH 2  and —COOH; a fatty diacid moiety with from 4 to 22 carbon atoms at least one free carboxylic acid group or a group which is negatively charged at neutral pH; and possible linkers which link the individual components in the side chain together via amide or ether bonds, said linkers optionally comprising a free carboxylic acid group in mixture with an insulin or an insulin analogue which has a rapid onset of action, together with a pharmaceutically acceptable carrier.