Enterococcus Antigens

Abstract

The present invention discloses isolated nucleic acid molecules encoding a hyperimmune serum reactive antigen or a fragment thereof as well as hyperimmune serum reactive antigens or fragments thereof from E. faecalis , methods for isolating such antigens and specific uses thereof.

1 - 37 . (canceled) 
     
     
         38 . A hyperimmune serum-reactive antigen comprising an amino acid sequence from any of SEQ ID NOs: 171-340 or 425-476 or fragments thereof. 
     
     
         39 . The hyperimmune serum-reactive antigen or fragment of  claim 38 , further defined as a peptide comprising an amino acid sequence described in: the “predicted immunogenic aa” and/or “location of identified immunogenic region” column of Table 1A or 1C or in Table 2. 
     
     
         40 . The hyperimmune serum reactive antigen or fragment of  claim 38 , further defined as comprising an amino acid sequence of amino acids: 4-10, 14-21, 30-36, 59-68, 77-82, 87-93, 96-105, 112-121, 125-133, 135-141, 150-162, 164-183, 192-203, 207-213, 215-226, 228-234, 241-247, 250-285, 302-308 and 135-148 of SEQ ID NO:171; 15-57, 60-73, 77-101, 108-134, 136-177, 185-201, 203-217, 226-240, 244-254, 272-277, 283-288, 292-343, 354-370, 380-398, 406-437, 439-453, 473-490, 532-538, 584-590, 595-601, 606-612, 664-677, 679-704, 715-724, 731-753, 759-772, 786-794, 814-862 and 657-684 of SEQ ID NO:172; 4-9, 15-36, 41-47, 54-60, 75-81, 114-120, 131-146, 152-158, 174-182, 194-202, 208-215, 218-226, 255-271, 276-285, 290-295, 302-311, 318-328, 330-344, 352-359, 365-377, 388-395, 398-405, 426-432, 439-449, 455-500, 505-513, 531-537, 542-552, 554-561, 587-595, 606-612, 718-734, 763-771, 775-782, 792-801, 805-812, 822-828, 830-843, 849-863, 876-894, 905-911, 919-926, 935-947, 949-958, 968-979, 1009-1016, 1029-1045, 1047-1056, 1076-1081, 1092-1106, 1123-1133, 1179-1200, 1202-1211, 1215-1223, 1287-1299, 1301-1306, 398-431 and 1224-1237 of SEQ ID NO:173; 17-47, 74-80, 90-97, 126-133, 137-148, 167-173, 179-185, 214-223, 250-255, 270-283, 329-338, 342-350, 352-358, 360-367, 372-383, 398-404, 411-421, 426-432, 435-446, 452-462, 472-479, 515-521, 582-592, 611-618, 623-629, 642-659, 666-673, 678-689, 704-725, 732-737, 744-757, 768-789, 824-834, 842-849, 862-868, 877-887, 904-916, 923-928, 941-947, 962-974, 982-992, 1019-1030, 1032-1044, 1046-1052, 1065-1075, 1077-1087, 1108-1121, 1124-1132, 1137-1151, 1170-1182, 1190-1206, 1208-1214, 1227-1233, 1242-1251, 1254-1273, 1282-1298 and 792-825 of SEQ ID NO:174; 19-31, 39-67, 82-91, 104-110, 113-128, 149-155, 161-181 and 137-155 of SEQ ID NO:175; 6-18, 54-63, 69-85, 110-127, 142-156, 158-167, 169-211, 238-246, 248-257, 276-311, 339-349, 371-380, 385-391, 394-403, 421-438, 451-456, 483-489 and 449-468 of SEQ ID NO:176; 5-15, 24-34, 50-56, 61-83, 98-121, 123-136, 149-162, 166-194, 202-215, 221-227, 229-332, 337-360, 367-402, 404-415, 427-433, 444-462, 471-478, 487-498, 511-518, 521-544, 550-563, 568-574, 580-587, 597-607, 610-616, 624-629 and 468-498 of SEQ ID NO:177; 11-19, 32-49, 57-63, 65-71, 80-89, 91-133, 166-181, 183-191, 201-230, 234-257, 264-291, 297-303, 305-314, 316-335, 337-354, 359-366, 368-374, 383-388, 394-405, 408-442, 446-470, 483-490, 499-505, 513-538, 544-555, 557-563, 568-590, 598-608, 617-623, 627-636, 641-647, 667-685, 687-693, 710-723, 733-739, 742-754, 769-815 and 366-388 of SEQ ID NO:178; 4-16, 30-35, 42-53, 67-76, 82-87, 101-108, 112-130, 132-138, 147-152, 161-183, 187-208, 218-225, 265-281, 295-303, 305-317, 322-334, 338-357, 360-368, 370-383, 387-394, 400-419, 421-430 and 255-336 of SEQ ID NO:179; 19-27, 36-47, 59-66, 76-83, 101-112, 118-125, 142-147, 162-180, 185-196, 225-240, 246-263, 286-304, 314-319, 327-333, 353-367 and 194-214 of SEQ ID NO:180; 14-43, 70-76, 83-89, 111-117, 122-128, 136-145, 163-170, 175-182, 210-219, 246-251, 266-279, 325-331, 338-346, 348-354, 356-363, 368-379, 422-428, 431-441, 450-456, 466-473, 509-515, 532-542, 549-556, 576-586, 605-612, 617-623, 636-653, 660-667, 674-686, 698-719, 726-731, 738-745, 762-783, 818-828, 836-843, 856-862, 871-881, 903-910, 917-922, 935-941, 956-968, 976-986, 1013-1024, 1026-1038, 1059-1069, 1071-1081, 1102-1115, 1118-1126, 1131-1145, 1164-1176, 1187-1200, 1202-1208, 1221-1227, 1236-1245, 1248-1267, 1273-1292, 252-287 and 805-844 of SEQ ID NO:181; 4-18, 21-28, 37-43, 56-70, 101-113, 131-140, 142-150, 162-170, 172-184, 193-204, 209-227, 233-238, 246-264 and 93-168 of SEQ ID NO: 182; 14-20, 44-50, 61-70, 77-96, 99-106, 129-142, 168-181, 187-196, 205-221, 225-241, 277-296 and 257-281 of SEQ ID NO:183; 18-29, 43-54, 64-76, 78-84, 88-103, 125-149, 159-176, 198-218, 230-242, 256-271, 279-285, 287-293, 300-306, 325-331, 344-351, 357-364, 371-397, 400-414, 419-464, 485-515, 517-526, 529-537, 548-553, 573-580, 584-590, 603-620, 639-661, 676-681, 687-700, 716-761, 772-780, 785-790, 795-803, 823-836, 848-853 and 106-134 of SEQ ID NO:184; 7-13, 19-42, 44-51, 55-75, 87-97, 99-110, 112-118, 129-135, 141-156, 158-178, 213-220, 230-286, 294-308, 323-338, 345-352, 355-365, 370-392, 394-419, 437-446, 454-460, 474-497, 515-526, 528-546, 569-575 and 128-141 of SEQ ID NO:185; 12-20, 24-33, 45-70, 73-84, 86-94, 103-116, 118-124, 135-142, 163-170, 176-200, 202-224, 226-234, 237-248, 250-262, 265-287, 296-307, 334-341, 347-356, 361-369, 382-396, 405-415, 418-427, 431-439, 443-449, 452-461, 467-474 and 113-146 of SEQ ID NO:186; 13-38, 44-50, 52-59, 66-72, 83-94, 103-110, 116-124, 131-137, 158-180, 199-204, 218-233, 241-264, 269-317, 326-342, 350-356 and 70-86 of SEQ ID NO:187; 29-35, 49-59, 63-84, 86-97, 103-111, 113-126, 130-144, 150-158, 174-198, 221-231, 250-264, 266-273, 291-298, 310-318 and 70-90 of SEQ ID NO:188; 19-25, 28-52, 60-66, 71-76, 131-142, 149-155, 157-178, 181-213, 218-223, 237-242, 250-257, 260-266, 272-279, 282-290, 321-330, 373-385, 393-407, 441-453, 461-475, 509-521, 529-542, 577-589, 597-610, 643-655, 663-677, 703-718, 729-734, 358-464, 495-570 and 604-685 of SEQ ID NO:189; 4-29, 51-76, 116-136, 158-173, 179-193, 207-215 and 86-111 of SEQ ID NO:190; 5-23, 45-70, 79-90, 93-107, 114-122, 142-151 and 18-36 of SEQ ID NO:191; 9-51, 68-120, 133-149, 158-180, 186-206, 211-220, 222-237, 248-293, 296-310, 317-339 and 248-260 of SEQ ID NO:192; 14-24, 44-63, 69-98, 108-119, 123-136, 155-161, 164-176, 180-193, 203-208, 215-223, 239-247, 274-281, 283-289, 296-304, 306-313, 315-327, 331-341, 343-353, 357-386, 392-405 and 205-246 of SEQ ID NO:193; 5-13, 16-23, 36-42, 53-63, 70-83, 96-102 and 14-34 of SEQ ID NO:194; 4-13, 19-35, 49-56, 59-76, 83-107, 121-134, 144-153, 157-164, 166-186, 194-202, 209-216, 231-253, 257-264 and 98-134 of SEQ ID NO:195; 16-32, 38-47, 58-68, 78-89, 98-114, 117-123, 132-141, 146-156, 164-170, 179-188, 196-212, 219-230, 232-237, 244-263, 265-274, 278-293, 297-303, 306-326, 339-349, 352-359, 362-367, 373-379, 384-394, 396-406, 423-443, 451-461, 465-484, 490-497, 504-511, 523-533, 537-547, 550-556, 558-566, 573-579, 586-593, 598-609, 615-642, 647-665, 671-686, 693-713, 723-728 and 332-378 of SEQ ID NO:196; 6-21, 34-44, 58-64, 66-74, 79-87, 114-127, 129-143, 154-162, 174-189, 205-214, 241-262, 266-273, 278-297, 319-324, 328-338, 342-351, 390-398, 409-415, 422-435, 458-464, 471-477, 481-486, 506-531, 534-540, 542-550 and 315-389 of SEQ ID NO:197; 4-28, 39-45, 52-58, 69-82, 93-115, 122-128, 135-140, 146-163, 177-192, 209-215, 221-232, 271-284, 331-337, 341-352, 360-378, 383-390, 392-401, 409-422, 428-435, 462-470, 474-480, 482-496, 531-539, 541-549, 551-560, 562-569, 576-582, 598-618 and 98-127 of SEQ ID NO:198; 14-27, 33-47, 61-79, 94-104, 119-133 and 36-60 of SEQ ID NO:199; 11-22, 29-40, 48-62, 68-73, 96-106, 108-118, 125-149 and 102-126 of SEQ ID NO:200; 4-11, 45-55, 76-83, 86-102, 105-112, 138-144, 147-153 and 20-48 of SEQ ID NO:201; 12-20, 28-56, 62-68, 72-82, 93-99, 101-107, 120-133, 135-145, 178-186, 208-232, 279-292 and 36-64 of SEQ ID NO:202; 6-14, 23-48, 65-82, 92-134, 140-181, 188-219, 228-238, 244-253, 255-261 and 124-145 of SEQ ID NO:203; 11-25, 31-38, 53-59, 62-71, 89-99, 125-133, 151-157, 182-190, 195-203, 208-215, 219-229, 249-262, 267-275, 287-295, 298-316, 318-325, 328-334, 344-353, 357-363, 371-377, 385-391, 396-415, 425-436, 438-457, 471-485, 538-552, 554-561, 606-625, 630-636, 646-653, 669-679, 695-704, 706-715, 722-747, 763-773 and 714-738 of SEQ ID NO:204; 10-29, 33-45, 50-60, 70-79, 83-95, 118-124, 136-157, 176-184, 192-205, 207-216, 223-234, 240-246, 258-268, 275-283 and 37-56 of SEQ ID NO:205; 4-24, 27-38, 46-54, 66-72, 81-97, 112-119, 128-137, 152-157, 173-179, 185-214, 219-225, 227-248, 262-284, 286-295, 301-307 and 117-134 of SEQ ID NO:206; 26-43, 49-56, 60-71, 74-82, 87-98, 110-116, 131-146, 154-164, 169-178, 183-189, 205-214, 241-246, 255-268, 275-292, 305-314, 316-323, 326-340, 346-363, 397-402, 419-429, 440-446, 452-461, 467-475 and 29-66 of SEQ ID NO:207; 7-16, 21-39, 48-58, 61-78, 82-89, 109-136, 138-150, 152-176, 182-247, 255-261, 267-332, 336-345, 347-358, 362-368, 371-392, 394-404, 407-472, 490-498, 505-513, 527-544, 554-582, 603-611, 614-620, 632-638 and 500-523 of SEQ ID NO:208; 24-46, 77-83, 90-97, 99-118, 123-166, 168-177, 204-212, 229-239, 248-262, 273-282, 287-293, 300-319, 321-337, 340-352, 357-366, 391-402, 411-428, 442-450, 464-471, 479-489 and 19-40 of SEQ ID NO:209; 9-23, 25-34, 53-58, 70-86, 90-97, 99-116, 118-128, 131-141, 185-191, 228-233, 237-253, 255-261, 264-271, 273-280, 302-312, 319-349, 351-359, 362-369, 376-383, 387-394, 398-406, 419-434 and 20-31 of SEQ ID NO:210; 15-22, 37-43, 71-87, 105-115, 121-127, 135-142, 152-158 and 32-52 of SEQ ID NO:211; 6-12, 18-29, 37-47, 50-58, 65-83, 85-91, 94-99, 108-123, 142-150, 156-163, 183-193, 215-222, 242-249, 252-258, 261-270, 285-308, 318-326 and 1-95 of SEQ ID NO:212; 9-61, 65-133, 144-155, 166-173, 175-221, 233-276, 278-313, 329-368 and 210-233 of SEQ ID NO:213; 11-29, 33-39, 46-51, 65-93, 107-113, 134-143, 147-154, 166-177, 181-188, 214-220, 233-243, 263-269 and 112-128 of SEQ ID NO:214; 8-46, 110-134, 155-167, 174-183, 188-201, 210-230, 253-258, 267-282, 289-299, 312-319, 322-327, 330-337, 365-381, 389-402, 405-411, 419-425, 439-447, 465-472, 489-512, 525-532, 540-554, 577-589, 591-599, 605-614, 616-624, 633-649 and 503-529 of SEQ ID NO:215; 34-49, 64-70, 90-118, 124-131, 141-152, 159-165 and 112-128 of SEQ ID NO:216; 5-15, 26-45, 55-72, 80-85, 93-100, 121-133, 142-148, 154-167, 198-205, 209-215, 241-254, 260-265, 271-279 and 244-270 of SEQ ID NO:217; 4-36, 38-54, 67-83, 122-153, 159-178, 205-212, 232-242, 244-253, 259-268, 281-288, 298-309, 324-331, 334-370, 372-381, 389-401, 403-429, 441-450, 456-462, 465-471, 473-479, 483-504, 508-518, 537-543, 553-565, 578-584, 592-609, 619-625, 658-667, 669-679, 712-719, 722-729, 737-744, 746-752, 758-765 and 180-226 of SEQ ID NO:218; 6-17, 23-32, 49-56, 61-67, 76-83, 85-103, 105-111, 120-132, 145-171, 175-185, 191-225, 231-246 and 99-128 of SEQ ID NO:219; 4-24, 28-48, 52-58, 64-79, 87-100, 104-120, 136-152, 159-166 and 150-163 of SEQ ID NO:220; 15-27, 65-71, 77-99, 104-121, 128-154, 183-216, 223-229, 234-255, 277-287, 296-308 and 77-97 of SEQ ID NO:221; 8-18, 44-76, 102-109 and 49-57 of SEQ ID NO:222; 5-14, 28-40, 42-51, 54-60, 77-83, 89-100, 117-124, 146-172, 176-204, 216-231, 237-244, 267-278, 324-334, 342-348, 396-401, 427-433, 438-450, 452-457, 465-471, 473-481, 491-500, 509-515, 523-544, 550-556, 558-569, 589-595, 606-618, 625-632, 640-649, 665-671, 678-688, 691-698, 717-723, 728-734, 781-789, 800-805, 812-821, 833-868, 873-879, 889-905, 929-939, 988-998, 1046-1061, 1073-1079, 1089-1096, 1115-1124, 1132-1140, 1172-1196, 1220-1226, 1231-1249, 1269-1277, 1287-1301, 1307-1330, 1350-1361, 1369-1378, 1387-1412, 1414-1420, 1422-1439, 1484-1491, 1513-1529, 1552-1561, 1576-1583, 1606-1613, 1617-1640, 1647-1654, 1665-1679, 1686-1698, 1709-1727, 1736-1743, 1750-1757, 1771-1790, 1801-1807, 1817-1823, 1831-1842, 1859-1868, 1870-1882, 1884-1891, 1900-1906, 1909-1914, 1929-1935, 1952-1960, 1974-1988, 2002-2011, 2032-2063, 2071-2081, 2116-2124, 2139-2147, 2149-2159, 2163-2190, 2209-2215, 2245-2253, 2282-2287, 2331-2342, 2360-2370, 2379-2393, 2402-2408, 2414-2421, 2423-2430, 2433-2439, 2442-2450, 2472-2478, 2485-2493, 2495-2503, 2506-2512, 2547-2554, 2558-2564, 2615-2625, 2637-2652, 2692-2698, 2700-2706, 2711-2723, 2731-2740, 2748-2753, 2756-2762, 2765-2772, 2781-2798, 2810-2824, 2844-2852, 2885-2899, 2912-2922, 2937-2944, 2947-2970, 2988-2998, 3016-3025, 3032-3037, 3062-3071, 3129-3148, 3156-3161 and 530-607 of SEQ ID NO:223; 31-36, 57-62, 79-85, 90-96, 99-112, 120-146, 162-185, 193-203, 208-217, 219-226, 239-253, 283-290, 298-304, 306-321, 340-349, 351-361, 365-372, 386-395, 407-438, 473-486, 537-551, 558-568, 576-594, 598-604 and 75-95 of SEQ ID NO:224; 14-19, 24-30, 34-42, 45-52, 54-64, 66-82, 95-105, 107-118, 126-163, 171-177, 184-201, 210-215, 260-269, 273-279, 288-304, 321-327, 358-364, 370-375, 380-387, 394-404, 407-413, 421-431, 436-451, 465-474, 504-511, 531-552, 578-587, 614-626, 629-636, 638-671, 691-715, 719-729, 733-745, 752-759, 768-777, 785-792, 794-802, 805-824, 844-854, 867-880, 885-891, 893-902, 907-924, 939-948, 955-964, 966-975, 987-1000, 1012-1017, 1023-1028, 1050-1071, 1083-1098, 1102-1115, 1133-1146, 1170-1183, 1204-1211, 1213-1223, 1262-1311, 1313-1319, 1346-1355, 1366-1371, 1383-1405, 1409-1414 and 776-819 of SEQ ID NO:225; 12-27, 30-38, 54-61, 64-74, 82-96, 103-110, 117-125, 134-140, 147-158, 185-201, 218-225, 232-253, 265-280, 319-325, 350-362, 366-372, 376-386, 464-483, 485-490, 511-521, 531-537, 542-559,564-574, 593-609, 613-619, 637-642, 668-677 and 195-214 of SEQ ID NO:226; 4-21, 59-67, 73-79, 84-91, 141-151, 186-197, 203-214, 222-227, 237-245, 255-260, 281-292, 294-311, 336-344, 346-355, 422-437, 459-466, 484-491 and 77-109 of SEQ ID NO:227; 10-45, 52-61, 63-70, 74-102, 112-122, 124-132, 164-178, 181-205, 212-240, 246-256 and 226-247 of SEQ ID NO:228; 38-50, 53-63, 78-87, 89-111, 126-152, 169-176, 179-186, 193-228, 254-267, 275-282, 288-304, 309-318, 325-341, 346-353, 358-367, 384-395, 404-427, 429-435, 456-465, 467-501, 510-521, 523-536, 541-548, 552-560, 563-584, 589-595, 597-620, 625-637, 639-645, 661-666, 712-729, 734-741, 743-750, 775-806, 809-816, 818-840, 842-850 and 693-714 of SEQ ID NO:229; 5-17, 30-37, 52-75, 77-86, 88-107, 112-135, 151-160, 178-222, 226-246, 263-270, 279-294, 306-314, 327-342, 345-352, 374-381, 389-416, 422-429, 435-449, 453-467, 473-500, 512-522, 524-531, 542-549, 552-560, 565-571, 575-586, 594-600, 613-619, 625-633, 635-641, 647-653, 667-674, 680-699, 711-729, 735-741, 764-775, 781-786,792-798, 805-813, 817-825, 833-842, 850-855, 860-866, 869-910, 917-930, 949-990 and 533-562 of SEQ ID NO:230; 7-14, 39-46, 61-74, 83-89, 93-99, 110-121, 136-150, 172-180, 182-200, 207-216, 223-236, 238-251, 265-271, 280-288, 294-309, 320-336, 339-354, 362-377, 383-389, 401-407, 435-441, 446-453, 460-465, 472-487, 499-511, 518-528, 533-540, 557-570, 572-587, 631-637, 643-658, 663-669, 672-678, 681-687, 695-706, 714-728 and 118-139 of SEQ ID NO:231; 5-19, 24-30, 56-64, 69-79, 93-100, 102-111, 117-123, 125-133, 174-182, 185-199, 205-224, 268-275, 311-336 and 102-125 of SEQ ID NO:232; 6-35, 39-45, 57-62, 80-85, 92-106, 117-122, 126-171, 214-223, 253-260, 268-273, 285-291, 295-306, 315-320, 325-336, 361-366 and 172-202 of SEQ ID NO:233; 4-13, 24-37, 45-51, 58-66, 84-92, 112-121, 132-141, 151-171, 175-195, 204-212, 222-240, 262-268, 276-295, 305-336, 338-348, 354-362 and 160-183 of SEQ ID NO:234; 10-16, 24-35, 41-73, 78-104, 111-121, 124-139, 141-148, 150-164, 196-215, 224-241, 249-282, 299-307, 315-357, 368-378, 393-401 and 345-367 of SEQ ID NO:235; 4-32, 48-53, 61-67, 84-104, 112-118 and 106-130 of SEQ ID NO:236; 21-28, 31-36, 65-81, 98-105, 115-121, 123-131, 136-142, 155-161, 177-190 and 201-232 of SEQ ID NO:237; 4-15, 21-27, 33-39, 42-56, 58-64, 68-82, 84-90, 92-98, 113-122, 146-162, 168-175, 177-189, 191-203, 249-268, 279-285, 287-304, 328-342, 349-358, 371-378, 387-393, 404-413, 419-425, 467-479, 487-498, 513-524, 528-539, 541-565, 572-579, 595-606, 626-635, 637-642 and 612-626 of SEQ ID NO:238; 7-13, 52-70, 76-82, 97-106, 110-117 and 13-45 of SEQ ID NO:239; 5-10, 12-48, 59-64, 87-102, 107-128, 131-140, 154-161, 165-171, 173-215 and 54-74 of SEQ ID NO:240; 4-11, 19-28, 34-40, 74-81, 87-98, 126-147, 163-171; 184-193, 205-213 and 49-124 of SEQ ID NO:241; 7-14, 23-29, 35-40, 61-67, 99-106, 111-122, 124-133, 135-161, 187-206, 216-229, 236-245, 262-268, 271-280 and 256-273 of SEQ ID NO:242; 4-13, 17-37, 47-54, 85-99, 105-113, 120-132, 147-166, 180-186, 192-199, 204-216 and 127-144 of SEQ ID NO:243; 14-27, 29-37, 52-62, 68-76, 89-96, 117-123, 125-131, 137-145, 166-195, 205-212, 214-222, 228-235, 258-264, 271-281, 288-296, 308-324, 332-339, 355-361, 365-371 and 268-293 of SEQ ID NO:244; 4-21, 30-42, 54-60, 78-85, 90-110, 141-147, 160-168, 176-185, 194-206, 218-225, 230-245, 251-261, 287-293, 295-304, 320-326, 334-347, 351-362, 386-402, 413-423, 427-433, 439-453, 456-477, 480-493, 507-513, 526-539, 574-581, 591-598, 600-609, 614-632, 655-665, 685-691, 703-712, 742-747, 757-775, 797-803, 813-819, 823-829, 880-887, 901-906, 930-944, 948-958, 962-968,971-995, 1002-1009, 1017-1023, 1036-1053, 1069-1081, 1107-1124, 1129-1152, 1178-1195, 1211-1223, 1249-1266, 1271-1288, 1334-1340, 1346-1367, 1-63 and 171-189 of SEQ ID NO:245; 4-22, 52-63, 70-75, 94-104, 112-125, 133-141, 176-199, 209-216, 244-259, 287-299, 336-352, 366-372, 386-399, 421-436, 444-449, 457-466, 481-487, 506-529, 531-540 and 295-378 of SEQ ID NO:246; 9-30, 43-49, 58-75, 86-96, 119-131, 138-147, 162-167, 181-201, 208-214 and 16-121 of SEQ ID NO:247; 4-27, 52-58, 80-90, 92-100, 108-114, 118-143, 169-176, 189-198, 247-261, 281-287, 307-317, 323-329, 352-363, 372-381, 396-411, 413-426, 429-440, 442-450, 456-461, 468-479 and 1-73 of SEQ ID NO:248; 4-32, 47-52, 57-63, 71-78, 92-104, 126-142, 153-175 and 145-163 of SEQ ID NO:249; 17-23, 35-41, 51-70, 73-86, 104-125 and 105-129 of SEQ ID NO:250; 25-32, 41-50, 75-85, 87-103, 115-122, 138-149, 164-171, 188-210, 212-220, 224-234, 256-273, 288-299, 304-310, 330-336, 357-365, 382-390, 399-405, 414-421, 440-446, 454-461, 480-486, 502-514, 518-540, 543-553, 561-567, 572-580, 582-588, 595-630, 633-651, 672-681, 691-709, 760-767, 813-832, 841-848, 852-866, 873-893, 919-925, 927-933, 940-955, 957-978, 984-997, 1000-1010, 1035-1040, 1044-1051, 1058-1064, 1081-1091, 1097-1124, 1129-1138, 1144-1150, 1158-1165, 1170-1180, 909-936 and 1001-1031 of SEQ ID NO:251; 4-12, 19-26, 31-41, 49-64, 66-86, 101-117, 119-127, 134-142, 152-161, 163-172, 179-188, 209-218, 234-241, 276-291, 294-300, 307-320, 324-341, 346-356, 373-387, 389-397, 410-416, 418-436, 444-454, 460-472, 481-486, 500-507, 511-535, 541-549, 553-559, 579-586, 602-607, 613-620, 628-640, 654-663, 671-678, 681-691, 709-722, 741-754, 766-774, 778-786, 797-803 and 212-226 of SEQ ID NO:252; 4-10, 15-27, 34-54, 60-73, 79-88, 101-115, 120-136, 154-162, 167-172, 222-240 and 126-195 of SEQ ID NO:253; 5-16, 18-25, 29-35, 57-63, 86-91, 107-121, 123-131, 170-179, 185-199, 204-226, 250-255, 262-274, 291-296, 325-347 and 1-38 of SEQ ID NO:254; 7-19, 22-34, 36-42, 48-54, 60-66, 71-76, 104-110, 118-133, 135-145, 158-164, 167-174, 182-193, 196-204, 217-229, 251-290, 293-299, 309-315 and 288-318 of SEQ ID NO:255; 43-51, 55-61, 66-73, 80-90, 103-127, 133-142, 174-180, 185-196, 203-210, 229-235, 239-251, 258-266, 272-278, 289-314, 316-326, 340-346, 355-361 and 14-27 of SEQ ID NO:256; 4-25, 27-33, 35-41, 52-74, 76-89, 99-124, 138-144, 146-159, 167-182, 184-191, 193-206, 211-223, 232-240, 249-257, 270-279, 281-287, 293-310, 322-341, 347-356 and 292-322 of SEQ ID NO:257; 5-13, 28-38, 43-60, 67-72, 98-116, 122-134, 137-151, 167-174, 177-195, 197-216 and 99-195 of SEQ ID NO:258; 15-33, 35-42, 48-57, 62-68, 73-91, 107-119, 121-153, 173-194, 205-210, 223-228, 234-241, 243-259, 275-298, 308-315, 327-340, 342-370, 376-391, 398-404, 410-419 and 71-122 of SEQ ID NO:259; 12-39, 43-64, 87-95, 99-105, 114-126, 128-136, 139-147, 212-225 and 107-141 of SEQ ID NO:260; 6-33, 40-45, 60-75, 79-86, 121-129, 131-137, 161-167, 172-178, 186-195, 203-212, 236-244, 257-264, 278-294, 306-312, 345-358, 368-381, 386-395, 404-410, 412-418 and 198-270 of SEQ ID NO:261; 18-31, 34-41, 50-56, 69-83, 99-106, 129-141, 147-153, 159-168, 170-178, 190-198, 200-212, 221-232, 237-255, 261-266, 274-292 and 118-216 of SEQ ID NO:262; 17-47, 61-67, 87-93, 115-121, 126-132, 140-148, 167-173, 179-186, 214-223, 250-255, 264-272, 282-294, 306-318, 338-353, 358-377, 385-401, 414-420, 433-441, 451-457, 470-480, 505-511, 544-550, 571-581, 600-607, 612-618, 631-648, 655-662, 669-681, 693-714, 721-726, 733-740, 757-778, 813-823, 831-838, 851-857, 866-876, 893-905, 912-917, 930-936, 951-963, 971-981, 1008-1019, 1021-1033, 1035-1041, 1054-1064, 1066-1076, 1097-1110, 1113-1121, 1126-1140, 1159-1171, 1182-1195, 1197-1203, 1216-1222, 1231-1240, 1243-1262, 1268-1287 and 738-828 of SEQ ID NO:263; 19-28, 40-46, 51-57, 68-74, 81-87, 98-108, 111-121 and 20-36 of SEQ ID NO:264; 4-17, 19-44, 60-69, 80-86, 110-116 and 33-60 of SEQ ID NO:265; 8-16, 18-28, 42-50, 53-75, 79-86, 94-99, 122-128, 136-142, 149-163, 166-173, 198-212, 254-272, 288-295, 304-318, 324-329, 343-348, 351-364, 367-383, 389-395, 411-420, 427-436 and 11-56 of SEQ ID NO:266; 19-25 and 6-24 of SEQ ID NO:267; 6-39, 59-68 and 44-63 of SEQ ID NO:268; 5-14, 21-28, 38-53 and 29-41 of SEQ ID NO:269; 4-13, 31-41, 56-65 and 32-56 of SEQ ID NO:270; 5-12 and 4-21 of SEQ ID NO:271; 4-18 and 17-32 of SEQ ID NO:272; 4-10, 23-33 and 14-30 of SEQ ID NO:273; 26-34, 44-53 and 35-52 of SEQ ID NO:274; 1-19 of SEQ ID NO:275; 4-17, 23-30, 32-37 and 6-23 of SEQ ID NO:276; 5-33, 40-58, 61-66 and 45-66 of SEQ ID NO:277; 15-41, 61-67 and 41-65 of SEQ ID NO:278; 4-12, 16-23, 26-37 and 10-29 of SEQ ID NO:279; 23-39 and 37-55 of SEQ ID NO:280; 12-20 and 38-55 of SEQ ID NO:281; 22-37 and 7-22 of SEQ ID NO:282; 3-14 of SEQ ID NO:283; 6-16, 43-65, 71-76 and 17-31 of SEQ ID NO:284; 4-13, 27-39, 42-69 and 17-32 of SEQ ID NO:285; 4-12, 26-39 and 10-25 of SEQ ID NO:286; 2-31 of SEQ ID NO:287; 6-38, 49-62 and 39-55 of SEQ ID NO:288; 4-10, 24-30 and 2-19 of SEQ ID NO:289; 12-17, 25-46 and 15-30 of SEQ ID NO:290; 4-13 and 2-28 of SEQ ID NO:291; 30-38 and 17-45 of SEQ ID NO:292; 24-33, 55-61 and 31-61 of SEQ ID NO:293; 4-26, 34-48 and 15-33 of SEQ ID NO:294; 9-15 and 1-22 of SEQ ID NO:295; 4-31 and 14-33 of SEQ ID NO:296; 5-34, 49-55, 64-82 and 69-83 of SEQ ID NO:297; 33-45 and 21-39 of SEQ ID NO:298; 7-14, 24-32, 42-65, 79-86 and 50-64 of SEQ ID NO:299; 13-27, 33-43, 45-62 and 12-37 of SEQ ID NO:300; 4-15, 17-32 and 10-26 of SEQ ID NO:301; 4-9, 11-43, 45-75 and 47-69 of SEQ ID NO:302; 4-18, 22-37 and 17-34 of SEQ ID NO:303; 4-14 and 5-24 of SEQ ID NO:304; 7-33, 35-46 and 1-19 of SEQ ID NO:305; 13-37, 69-75 and 51-69 of SEQ ID NO:306; 14-24, 26-34, 37-49, 66-78 and 2-25 of SEQ ID NO:307; 17-46, 52-57, 59-64 and 54-68 of SEQ ID NO:308; 4-22 and 13-25 of SEQ ID NO:309; 8-40, 53-63 and 29-50 of SEQ ID NO:310; 16-28 and 32-40 of SEQ ID NO:311; 14-20, 22-28, 39-45 and 2-22 of SEQ ID NO:312; 4-13 and 12-31 of SEQ ID NO:313; 15-21 and 2-17 of SEQ ID NO:314; 4-17 and 20-36 of SEQ ID NO:315; 4-19 and 9-18 of SEQ ID NO:316; 4-14 and 3-19 of SEQ ID NO:317; 4-21, 32-40 and 21-39 of SEQ ID NO:318; 4-13 and 10-27 of SEQ ID NO:319; 18-31, 39-47, 75-87, 89-98 and 79-99 of SEQ ID NO:320; 15-21 and 9-24 of SEQ ID NO:321; 4-14, 18-27, 30-53, 55-64, 68-74, 81-98 and 22-40 of SEQ ID NO:322; 7-24, 44-51 and 35-60 of SEQ ID NO:323; 10-47 and 23-37 of SEQ ID NO:324; 4-10, 12-46 and 7-22 of SEQ ID NO:325; 20-27 and 1-13 of SEQ ID NO:326; 6-19, 41-51 and 9-37 of SEQ ID NO:327; 4-9, 11-17 and 9-23 of SEQ ID NO:328; 4-17, 23-38, 46-66, 68-85 and 34-46 of SEQ ID NO:329; 4-18, 34-59, 75-81 and 61-84 of SEQ ID NO:330; 6-17 and 7-28 of SEQ ID NO:331; 4-32, 56-61 and 35-52 of SEQ ID NO:332; 4-14, 27-71, 74-88, 93-110, 115-120, 124-130, 139-154, 161-172 and 146-171 of SEQ ID NO:333; 4-21 and 3-15 of SEQ ID NO:334; 12-17 and 9-26 of SEQ ID NO:335; 10-21, 45-58 and 51-67 of SEQ ID NO:336; 59-66, 68-84 and 13-42 of SEQ ID NO:337; 11-16 and 1-16 of SEQ ID NO:338; 4-19, 23-37 and 10-30 of SEQ ID NO:339; 19-27, 35-46, 48-66, 82-88, 99-105, 113-119 and 42-59 of SEQ ID NO:340; 135-147 of SEQ ID NO:171; 658-682 of SEQ ID NO:172; 411-427 and 1226-1246 of SEQ ID NO:173; 794-817 and 801-824 of SEQ ID NO:174; 468-492 and 474-495 of SEQ ID NO:177; 366-388 of SEQ ID NO:178; 266-291, 287-312 and 308-333 of SEQ ID NO:179; 197-213 and 195-211 of SEQ ID NO:180; 252-275, 262-285 and 812-830 of SEQ ID NO:181; 94-112, 97-120 and 104-128 of SEQ ID NO:182; 257-281 of SEQ ID NO:183; 106-134 of SEQ ID NO:184; 70-86 of SEQ ID NO:187; 358-383, 378-402, 397-421, 499-524, 520-545, 541-566, 622-646, 641-665 and 660-684 of SEQ ID NO:189; 248-260 of SEQ ID NO:192; 15-34 of SEQ ID NO:194; 112-129 of SEQ ID NO:195; 333-358 and 353-378 of SEQ ID NO:196; 316-343, 339-366 and 362-389 of SEQ ID NO:197; 98-123 and 104-126 of SEQ ID NO:198; 20-43 and 23-48 of SEQ ID NO:201; 124-145 of SEQ ID NO:203; 717-738 of SEQ ID NO:204; 37-56 of SEQ ID NO:205; 118-134 of SEQ ID NO:206; 500-522 of SEQ ID NO:208; 32-47 of SEQ ID NO:211; 25-51, 47-73 and 69-95 of SEQ ID NO:212; 503-529 of SEQ ID NO:215; 112-128 of SEQ ID NO:216; 181-199 of SEQ ID NO:218; 109-121 of SEQ ID NO:219; 150-163 of SEQ ID NO:220; 77-97 of SEQ ID NO:221; 564-586 of SEQ ID NO:223; 75-94 of SEQ ID NO:224; 776-798, 784-808 and 794-815 of SEQ ID NO:225; 196-212, 78-100 and 85-107 of SEQ ID NO:226; 536-553 of SEQ ID NO:230; 102-125 of SEQ ID NO:232; 178-198 of SEQ ID NO:233; 612-626 of SEQ ID NO:238; 171-187 of SEQ ID NO:245; 296-320, 315-339, 334-358 and 353-377 of SEQ ID NO:246; 47-71 of SEQ ID NO:247; 1-25, 20-45 and 40-64 of SEQ ID NO:248; 146-161 of SEQ ID NO:249; 910-935 and 1007-1030 of SEQ ID NO:251; 212-226 of SEQ ID NO:252; 126-152, 148-173 and 169-195 of SEQ ID NO:253; 288-310 and 293-316 of SEQ ID NO:255; 293-312 of SEQ ID NO:257; 154-170 of SEQ ID NO:258; 72-95, 90-112 and 97-121 of SEQ ID NO:259; 135-150 and 146-163 of SEQ ID NO:262; 799-827 of SEQ ID NO:263; 23-43 and 33-53 of SEQ ID NO:266; 44-62 of SEQ ID NO:268; 6-22 of SEQ ID NO:276; 37-54 of SEQ ID NO:280; 40-54 of SEQ ID NO:281; 7-21 of SEQ ID NO:282; 4-11, 16-34, 48-55, 67-77, 87-106 and 153-183 of SEQ ID NO:425; 22-40, 49-65, 70-91, 95-109, 111-125, 146-207, 209-216, 219-225, 229-244, 251-270, 274-286, 292-309, 316-329, 335-355, 358-370, 376-388, 392-419, 425-430, 435-441, 448-455, 464-478, 486-515 and 437-465 of SEQ ID NO:426; 5-19, 25-31, 43-48, 60-79, 88-100, 105-129, 148-171, 187-193, 243-263, 316-322, 334-340, 345-351, 369-378, 381-391, 399-404, 474-483, 502-517, 525-530, 558-568, 579-596, 622-627, 631-638, 644-651, 653-660, 674-680, 687-693, 721-728, 743-753, 760-775, 788-795, 806-813, 821-828, 835-842, 847-859, 868-887 and 300-347 of SEQ ID NO:427; 5-26, 37-44, 89-97, 112-118, 121-128, 138-154, 157-165, 176-181, 188-198, 205-218, 223-243, 247-253, 260-279 and 76-155 of SEQ ID NO:428; 4-29, 41-46, 49-68, 82-88, 121-147, 158-164, 187-193, 195-208, 229-236, 244-249, 251-263, 269-275, 307-313, 337-343, 348-381, 392-398, 402-408, 432-438, 85-117 and 194-239 of SEQ ID NO:429; 5-12, 14-22, 28-34, 40-46, 70-79, 84-129, 152-165, 174-182 and 37-109 of SEQ ID NO:430; 5-16, 18-52, 54-72, 81-86, 118-126, 136-145, 151-157, 168-180, 209-233, 244-270, 295-302, 315-326, 329-337, 345-352, 364-373, 397-402, 408-418, 424-431, 435-443, 472-480, 483-489, 504-510, 519-527, 549-564, 576-599, 605-637, 641-673 and 91-98 of SEQ ID NO:431; 23-36, 42-52, 133-140, 151-157, 242-247, 267-277, 295-301, 320-328, 333-339, 345-352, 365-371, 397-403, 415-428, 456-465, 481-487, 489-495, 508-516, 518-527, 585-592, 606-614, 631-637, 643-658, 665-670, 723-728, 737-744, 752-759, 787-793, 835-841, 873-885, 918-928, 938-945, 951-966, 978-988, 1015-1020, 1030-1036, 1044-1052, 1058-1069, 1071-1079, 1081-1088, 1113-1119, 1125-1138, 1141-1147, 1164-1170, 1172-1177, 1190-1200, 1214-1220, 1230-1236, 1239-1245, 1262-1268, 1270-1275, 1288-1298, 1312-1318, 1328-1334, 1337-1343, 1360-1366, 1368-1373, 1386-1396, 1410-1416, 1426-1432, 1435-1441, 1458-1464, 1466-1471, 1484-1494, 1508-1514, 1524-1530, 1533-1539, 1556-1562 and 307-340 of SEQ ID NO:432; 19-25, 35-41, 44-50, 66-72, 74-79, 92-102, 116-122, 132-138, 141-147, 164-170, 172-177, 190-200, 214-220, 230-236, 239-245, 262-268, 270-275, 288-298, 312-318, 328-334, 337-343, 360-366, 368-373, 386-396, 410-416, 426-432, 435-441, 458-464, 466-478, 504-524, 79-148, 177-246, 275-344 and 373-442 of SEQ ID NO:433; 7-14, 16-23, 33-39, 46-53, 72-79, 92-115, 123-130, 156-175, 179-187, 214-220, 239-246, 266-274, 302-325, 338-354, 360-370, 375-390, 392-401, 421-428, 430-463 and 29-58 of SEQ ID NO:434; 4-9, 22-39, 58-65, 72-82, 87-92, 99-104, 107-119, 143-166, 171-177, 194-202, 205-213, 220-228, 231-240, 247-263, 309-315, 317-323, 336-343 and 294-320 of SEQ ID NO:435; 4-10, 12-18, 24-29, 34-43, 50-65, 70-76, 111-117, 129-138, 152-159, 166-171, 184-195, 200-210, 224-236, 241-251, 274-283, 285-296, 313-319, 332-341, 348-355, 378-386, 410-416, 433-445, 475-482, 523-529, 531-540, 584-596, 626-633, 674-680, 682-688, 738-750, 780-787, 828-834, 836-842, 853-862, 882-887, 893-912 and 604-676 of SEQ ID NO:436; 15-38, 49-57, 60-99, 103-119, 124-194, 200-206, 215-249, 251-291, 307-313, 315-347, 369-378, 383-390, 393-400, 405-411, 423-435, 440-447, 454-460, 470-486, 490-503, 532-539, 542-549, 551-567, 579-592 and 509-583 of SEQ ID NO:437; 38-44, 47-88, 95-103, 157-172, 235-240, 250-260, 263-276, 294-300, 312-317, 331-337, 369-391, 412-419, 442-448, 453-463, 490-529, 537-555, 571-580, 600-617, 619-627, 642-648, 682-687, 693-700, 716-722, 738-748, 756-763, 779-789, 796-802, 820-828, 833-840, 846-853, 862-872, 880-887, 894-899, 924-937, 957-963, 1006-1012, 1043-1049, 1063-1069, 1076-1097 and 124-147 of SEQ ID NO:438; 4-28, 31-49, 60-71, 75-102, 104-114, 134-144, 160-184, 250-257, 277-285, 287-294, 330-338, 345-351, 367-374, 381-388, 393-399, 402-407, 420-426, 443-448, 458-464, 411-436 and 454-488 of SEQ ID NO:439; 20-27, 45-55, 57-64, 66-77, 98-106, 130-137, 155-165, 167-174, 176-187, 194-203, 208-223, 227-238, 245-251, 257-270, 273-278, 287-299, 330-345, 352-358, 363-385, 392-399, 410-417, 437-443, 467-484, 486-492, 495-500, 504-516, 526-536 and 219-270 of SEQ ID NO:440; 11-22, 24-31, 46-63, 65-71, 73-88, 95-109, 174-181, 183-201, 204-212, 216-222, 228-233, 241-247 and 142-221 of SEQ ID NO:441; 8-28, 51-59, 67-84, 93-98, 140-152, 154-162, 183-188 and 91-125 of SEQ ID NO:442; 10-22, 27-61 and 69-100 of SEQ ID NO:443; 7-15, 18-26, 94-100, 126-131, 152-165, 219-228, 254-263, 274-292, 297-308, 333-340, 342-352, 354-371, 373-379, 403-410, 420-438, 450-456, 463-470, 489-495, 503-512 and 97-173 of SEQ ID NO:444; 4-21, 37-43, 49-65, 67-74, 76-90, 113-119, 131-141, 155-173, 175-189, 192-199, 207-221, 247-254, 266-276, 317-322, 337-343, 387-393, 408-428, 439-448, 451-460, 469-479, 482-487, 493-501, 517-523, 533-542 and 480-503 of SEQ ID NO:445; 11-26, 40-46, 78-86, 93-103, 121-126, 132-138, 166-177, 183-196, 203-212, 214-221, 228-263, 304-311, 323-338, 345-351, 357-363, 379-393, 420-434, 442-448, 518-527, 547-553, 581-591, 602-609, 637-645, 665-674, 687-692, 701-708, 730-739, 796-802, 844-857, 882-888, 903-914, 944-950, 976-983, 1027-1033, 1049-1057, 1066-1072, 1085-1092, 1120-1127, 1137-1144, 1153-1158, 1165-1176, 1181-1187, 1221-1230, 1238-1244, 1269-1274 and 605-632 of SEQ ID NO:446; 6-47, 57-65, 83-95, 109-121, 138-147, 154-164, 167-177, 194-200, 202-212, 227-234, 240-253, 260-267, 283-291, 320-329, 340-347, 356-364, 412-422, 430-436, 441-459, 465-475, 478-486, 498-507 and 59-84 of SEQ ID NO:447; 10-21, 58-83, 88-97, 120-126 and 21-51 of SEQ ID NO:448; 5-39, 56-62, 76-88, 90-114, 138-162, 170-195, 202-221, 228-250, 264-270, 304-355, 374-387, 391-416, 462-471, 526-546, 554-561, 574-579, 639-645, 651-660, 674-682, 689-694 and 666-697 of SEQ ID NO:449; 6-30, 36-42, 143-157, 176-197, 202-209, 216-233, 241-246, 275-287, 292-299, 315-325, 343-350, 375-380, 397-403, 411-420, 422-434, 441-448, 467-474, 477-499, 555-568, 591-597, 601-609, 623-644, 667-688, 692-698, 703-718, 736-747, 757-766, 782-791, 795-801, 832-840, 859-865 and 226-269 of SEQ ID NO:450; 6-23, 43-51, 61-67, 73-82, 91-97, 123-130, 149-158, 164-175, 228-234, 240-246, 248-255, 262-272, 326-332, 340-347, 365-371, 377-388, 409-419, 425-431, 438-445, 449-457, 464-470, 496-507, 559-568, 575-581, 603-608, 617-623, 633-639, 648-654, 659-670, 695-701, 734-752, 806-814, 816-829, 861-868, 891-899, 904-909, 937-945, 947-960, 978-983, 992-999, 1022-1031, 1068-1076, 1078-1091, 1109-1114, 1123-1130, 1153-1162, 1199-1207, 1209-1222, 1254-1261, 1284-1293, 1330-1338, 1340-1353, 1371-1376, 1385-1392, 1415-1421, 1433-1438, 1460-1465, 1470-1492 and 1422-1458 of SEQ ID NO:451; 82-94, 111-118, 125-131, 206-212, 261-266, 310-320, 328-338, 345-351, 353-360, 414-420, 424-434, 440-447, 451-500, 506-516, 548-561, 566-572, 584-591, 601-622, 630-636, 650-659, 661-667, 674-699, 703-711, 717-729, 736-744, 752-759, 765-771, 813-822, 826-842, 852-868, 870-877, 881-895, 897-906, 913-922 and 602-671 of SEQ ID NO:452; 12-18, 20-25, 43-54, 56-65, 73-79, 82-88, 99-111, 136-142, 153-169, 171-183, 195-223, 229-248, 255-260, 272-277, 281-292, 298-319, 322-329, 332-351, 363-379, 381-389 and 275-304 of SEQ ID NO:453; 4-9, 34-48, 65-77, 101-106, 111-131, 138-153, 186-191, 230-250 and 148-219 of SEQ ID NO:454; 4-23, 30-35, 42-53, 67-76, 82-87, 101-108, 112-130, 132-138, 147-152, 161-183, 187-208, 218-225, 265-283, 295-303, 306-317, 322-334, 338-357, 360-368, 370-383, 387-398, 400-419, 421-430, 104-182 and 240-304 of SEQ ID NO:455; 4-12, 63-69, 94-102, 146-164, 166-173, 175-181, 193-207, 263-281, 286-295, 301-306, 330-343, 369-378, 382-388, 414-420, 422-430, 438-454, 456-462, 472-531, 543-560, 581-591, 596-605, 614-623, 626-635, 656-662, 669-676, 683-690, 693-698, 705-711, 728-736, 752-764 and 69-102 of SEQ ID NO:456; 6-12, 43-53, 141-147, 164-179, 185-195, 197-206, 227-235, 237-271, 288-305, 308-317, 335-341, 351-357, 365-376, 386-395, 397-416, 422-447 and 11-35 of SEQ ID NO:457; 16-24, 50-65, 73-84, 88-99, 114-124, 130-146, 181-187, 193-203, 214-220, 236-247, 250-258, 287-297 and 50-113 of SEQ ID NO:458; 4-25, 50-55, 76-82, 117-123, 131-137, 139-148, 157-166, 239-245, 253-258, 266-275, 277-292, 300-306, 51-83 and 93-161 of SEQ ID NO:459; 6-22, 34-43, 51-86, 93-100, 110-116, 150-161, 164-171, 180-187, 197-218 and 168-237 of SEQ ID NO:460; 4-27, 55-60, 74-82 and 10-46 of SEQ ID NO:461; 6-19, 25-31, 43-49, 60-79, 88-100, 105-129, 148-161, 164-171, 187-193, 243-263, 316-322, 334-340, 369-378, 381-391, 398-404, 460-466, 474-483, 502-509, 511-517, 525-530, 558-567, 579-596, 622-627, 631-638, 641-651, 653-659, 674-680, 687-693, 710-716, 720-727, 743-753, 760-775, 788-795, 806-813, 821-828, 836-842, 847-860, 865-880 and 258-377 of SEQ ID NO:462; 4-11, 25-64, 71-79, 88-94, 107-120, 123-132, 167-188, 231-237, 240-246, 261-267, 306-311, 330-342, 351-358, 389-395, 406-418, 429-434, 439-448, 483-501, 511-520 and 71-143 of SEQ ID NO:463; 4-18, 22-27, 53-64, 94-100, 121-127, 133-139, 155-164, 177-182, 187-196, 206-218, 224-242, 248-253, 258-277 and 184-253 of SEQ ID NO:464; 10-17, 56-67, 72-82, 94-99, 106-113, 166-173, 229-235, 243-283, 295-301, 313-321, 326-331, 342-348, 396-414, 423-435, 446-452, 454-462, 496-502, 511-534, 543-556, 563-570, 586-593, 616-626, 638-645, 653-662, 679-696, 731-737, 766-774, 776-782, 790-796, 810-817, 825-835, 837-846 and 540-615 of SEQ ID NO:465; 13-24, 30-36, 73-81, 89-95, 109-115, 117-143, 161-173, 179-189, 226-244, 251-261, 275-281, 298-305, 307-315, 323-328, 364-374, 69-186 and 264-354 of SEQ ID NO:466; 19-25 and 6-22 of SEQ ID NO:467; 6-39, 59-68 and 43-62 of SEQ ID NO:468; 6-14, 22-32 and 1-27 of SEQ ID NO:469; 4-41 and 28-40 of SEQ ID NO:470; 8-14 and 4-19 of SEQ ID NO:471; 4-10, 12-22, 30-35 and 6-33 of SEQ ID NO:472; 4-16, 24-33 and 37-54 of SEQ ID NO:473; 2-23 of SEQ ID NO:474; 4-21, 27-33, 36-41 and 14-34 of SEQ ID NO:475; or 4-14, 24-30, 37-42, 57-78, 83-89, 94-103, 113-131 and 100-122 of SEQ ID NO:476. 
     
     
         41 . The hyperimmune serum-reactive antigen or fragment of  claim 38 , comprising at least 6 contiguous amino acids of any of SEQ ID NOs: 171-340 or 425-476. 
     
     
         42 . The hyperimmune serum-reactive antigen or fragment of  claim 38 , comprising at least 8 contiguous amino acids of any of SEQ ID NOs: 171-340 or 425-476. 
     
     
         43 . The hyperimmune serum-reactive antigen or fragment of  claim 38 , comprising at least 10 contiguous amino acids of any of SEQ ID NOs: 171-340 or 425-476. 
     
     
         44 . The hyperimmune serum-reactive antigen or fragment of  claim 38 , further defined as directed against enterococcal infection. 
     
     
         45 . A pharmaceutical composition comprising a hyperimmune serum-reactive antigen or fragment of  claim 38 . 
     
     
         46 . The pharmaceutical composition of  claim 45 , wherein the hyperimmune serum-reactive antigen or fragment is directed against  E. faecalis.    
     
     
         47 . The pharmaceutical composition of  claim 45 , further defined as comprising at least two different hyperimmune serum-reactive antigens and/or fragments. 
     
     
         48 . The pharmaceutical composition of  claim 47 , wherein the at least two different hyperimmune serum-reactive antigens and/or fragments are both directed against  E. faecalis.    
     
     
         49 . The pharmaceutical composition of  claim 45 , further comprising an immunostimulatory substance. 
     
     
         50 . The pharmaceutical composition of  claim 49 , wherein the immunostimulatory substance is a polycationic polymer, an immunostimulatory deoxynucleotide (ODN), a peptide containing at least two LysLeuLys motifs, a neuroactive compound, alum, or a Freund's complete or incomplete adjuvant. 
     
     
         51 . The pharmaceutical composition of  claim 50 , wherein the polycationic polymer is a polycationic peptide. 
     
     
         52 . The pharmaceutical composition of  claim 50 , wherein the neuroactive compound is human growth hormone. 
     
     
         53 . The pharmaceutical composition of  claim 45 , further defined as a vaccine. 
     
     
         54 . The pharmaceutical composition of  claim 53 , further defined as a vaccine for treatment and/or prevention of enterococcal infection. 
     
     
         55 . A method of vaccinating a subject comprising:
 obtaining a pharmaceutical composition of  claim 45 ; and   administering the pharmaceutical composition to a subject;   wherein the subject is vaccinated.   
     
     
         56 . The method of  claim 55 , wherein the subject is a human. 
     
     
         57 . The method of  claim 55 , further defined as a method of treating and/or preventing enterococcal infection in the subject. 
     
     
         58 . The method of  claim 55 , wherein the hyperimmune serum-reactive antigen or fragment is directed against  E. faecalis.    
     
     
         59 . The method of  claim 55 , wherein the pharmaceutical composition comprises at least two different hyperimmune serum-reactive antigens or fragments thereof. 
     
     
         60 . The method of  claim 59 , wherein the at least two different hyperimmune serum-reactive antigens or fragments thereof are both directed against  E. faecalis.