Gene Coding for Glucose-6-Phosphate-Dehydrogenase Proteins

Abstract

The present invention relates to isolated nucleic acid molecules encoding mutants of glucose-6-phosphate dehydrogenase, and vectors and hosts cells including such nucleic acid molecules. These nucleic acid molecules are involved in the biosynthesis of a fine chemical, e.g., an amino acid such as lysine. The present invention also relates to methods of producing and modulating the production of fine chemicals, e.g., lysine, by culturing recombinant microorganisms containing these nucleic acid molecules under conditions such that the fine chemical is produced.

1 . An isolated nucleic acid molecule, coding for a polypeptide having the amino acid sequence referred to in each case in Table 1/column 2, wherein the nucleic acid molecule in the amino acid position indicated in table 1/column 4 encodes a proteinogenic amino acid different from the particular amino acid indicated in Table 1/column 5 in the same row. 
     
     
         2 . An isolated nucleic acid molecule as claimed in  claim 1 , wherein the nucleic acid molecule in the amino acid position indicated in Table 1/column 4 encodes the amino acid indicated in Table 1/column 6 in the same row. 
     
     
         3 . A vector, which comprises at least one nucleic acid sequence as claimed in  claim 1 . 
     
     
         4 . A host cell, which is transfected with at least one vector as claimed in  claim 3 . 
     
     
         5 . A host cell as claimed in  claim 4 , wherein expression of said nucleic acid molecule modulates the production of a fine chemical from said cell. 
     
     
         6 . A method for preparing a fine chemical, which comprises culturing a cell which has been transfected with at least one vector as claimed in  claim 3  so that the fine chemical is produced. 
     
     
         7 . A method as claimed in  claim 6 , wherein the fine chemical is an amino acid. 
     
     
         8 . A method as claimed in  claim 7 , wherein said amino acid is lysine.