Methods for diagnosing and treating hemostatic disorders by modulating p-selectin activity

Abstract

The present invention identifies P-selectin as a modulator of hemostasis. Accordingly, the present invention relates to methods for the identification and use of modulators of P-selectin activity as modulators of hemostasis. The invention also relates to methods and compositions for the diagnosis and treatment of hemostatic disorders, including, but not limited to, hemorrhagic disorders and thrombotic disorders. The present invention describes methods for the diagnostic evaluation and prognosis of various hemostatic conditions, and for the identification of subjects exhibiting a predisposition to such conditions. In addition, the present invention provides methods for the diagnostic monitoring of patients undergoing clinical evaluation for the treatment of a hemostatic or vascular disorders, and for monitoring the efficacy of compounds in clinical trials.

1 - 41 . (canceled) 
     
     
         42 . A method for diagnosing a procoagulant state in a subject, comprising determining a P-selectin activity in a biological sample of the subject, wherein an increased P-selectin sample indicates a procoagulant state in the subject. 
     
     
         43 . The method of  claim 42 , which comprises providing a test sample of blood from a subject and comparing the level of soluble P-selectin in the test sample to the level of soluble P-selectin in a control blood sample from a subject with normal hemostatic activity, wherein an increased level of soluble P-selectin in the test sample as compared to the control sample is an indication of a procoagulant state in the subject. 
     
     
         44 - 49 . (canceled) 
     
     
         50 . The method of  claim 42 , wherein the agent used to determine the P-selectin activity is a labeled P-selectin antibody.