Intracoronary Device And Method Of Use Thereof

Abstract

Engraftment of therapeutic cells and agents to a target site in an organism is enhanced by mechanical, chemical and biological methods and systems.

1 . A method of enhancing engraftment of therapeutic cells at a target site in a mammal, comprising contacting the therapeutic cells with a biological linker, wherein the linker is attached to the cell membrane of the therapeutic cells, and wherein at least one functionality of the linker molecule has affinity to the surface of the therapeutic cells, and wherein at least one other functionality of the linker has affinity to the surface of the lumen surface of the target area vasculature. 
     
     
         2 . The method of  claim 1 , wherein the linker is irreversibly attached to the therapeutic cells. 
     
     
         3 . The method of  claim 1 , wherein the linker is reversibly attached to the therapeutic cells. 
     
     
         4 . The method of  claim 1 , wherein the linker is a biological conjugate. 
     
     
         5 . The method of  claim 1 , wherein the linker is a multifunctional linker. 
     
     
         6 . The method of  claim 1 , wherein the linker is a bi-functional linker. 
     
     
         7 . The method of  claim 1 , wherein the linker molecule comprises at least one of an antibody, an antibody fragment, a peptide, or an affibody. 
     
     
         8 . The method of  claim 1 , wherein the functionalities may be separated by a spacer. 
     
     
         9 . The method of  claim 8 , wherein the spacer is a hydrophilic polymer. 
     
     
         10 . The method of  claim 9 , wherein the spacer is PEG. 
     
     
         11 . The method of  claim 8 , wherein the spacer has branches or is of star form. 
     
     
         12 . The method of  claim 1 , wherein the linker comprises linked antibodies, fragments of antibodies (F ab  fragments), affibodies, peptides or other molecules with affinity to receptor molecules on the target surface.