Image Processing Method for a Microscope System

Abstract

An embodiment is disclosed for performing the image processing for analyzing the results of a fluorescence in situ hybridization (FISH) microscopic automated sample analysis to determine specific chromosomal characteristics.

1 . A computer-usable medium having computer readable instructions stored thereon for execution by a processor to perform a method comprising:
 adjusting a microscope to bring a specimen into focus at a focal plane within the depth of the said specimen;   irradiating the said specimen with fluorescence exciting illumination;   adjusting the exposure parameters of an electronic imaging device;   capturing the image of the said specimen with said electronic imaging device;   enumerating objects-of-interest;   identifying nuclei;   segmenting said nuclei;   counting and characterizing the color of bright fluorescent light signals   occurring within said nuclei; and   interpreting and reporting the results.