Phytase Variants

Abstract

The present invention relates to phytase variants, their preparation and uses, which phytase variants, when aligned according to FIG. 1 , are amended as compared to a model phytase in at least one of a number of positions. Preferred model phytases are basidiomycete and ascomycete phytases, such as Peniophora phytase and Aspergillus phytases. Preferred phytase variants exhibits amended activity characteristics, such as improved specific activity and/or improved thermostability.

1 - 85 . (canceled) 
     
     
         86 . A modified phytase comprising a mutation in an amino acid sequence of a phytase, wherein the modified phytase has phytase activity and the mutation is at one or more positions selected from the group consisting of:
 24; 27; 31; 33; 39; 40; 41; 42; 46; 49; 56; 59; 68; 69; 70; 71; 72; 73; 74; 75; 76; 77; 78; 81; 82; 84; 116; 117; 119; 120; 121; 122; 123; 124; 125; 127; 128; 132; 149; 150; 151; 152; 155; 156; 157; 158; 159; 160; 161; 162; 163; 170f; 170g; 171; 184; 185; 187; 190; 191; 192; 193; 194; 200; 201; 201a; 201b; 201c; 201d; 201f; 202; 223; 228; 232; 233; 235; 236; 237; 239; 243; 246; 253; 256; 271; 272; 274; 275; 276; 277; 279; 280; 283; 285; 287; 288; 292, 293; 304; 332; 333; 334; 335; 336; 338; 341; 342; 343; 348; 349; 362; 364; 367; 368; 369; 370; 371; 372; 374; 375; 376; 387; 393; 394; 396; 409; 412; 413; 421; and 431,   wherein each position corresponds to the position of the amino acid sequence of the mature  P. lycii  phytase (SEQ ID NO: 7).   
     
     
         87 . The modified phytase of  claim 86 , wherein the mutation is selected from the group consisting of:
 24C; 27P; 31Y; 33C; 39H,S,Q; 40L,N; 42S,G; 49P; 56P; 58D,K,A; 59G; 69Q; 75W,F; 78D,S; 81A,G,Q,E; 82T; 84I,Y,Q,V; 116S; 119E; 120L; 122A; 123N,Q,T; 125M,S; 127Q,E,N; 128A,S,T; 132F,I,L; 151A,S; 152G; 157V; 158D,A; 159T; 160A,S; 161T,N; 162N; 163W; 170fH; 170gA; 171N; 184Q,S,P; 185S; 187A; 190A,P; 193S; 194S,T; 200G,V; 201D,E; 201a( ); 201b( ); 201c( ); 201d( ); 201f( ); 202S,A; 223H,D; 228N; 232T; 233E; 235Y,L,T; 236Y,N; 237F; 246V; 253P; 256D; 271D,N; 275F,Y; 280A,P; 283P; 287A,T; 288L,I,F; 292F,Y; 293A,V; 304P,A; 332F; 336S; 338I; 343A,S,F,I,L; 348Y; 349P; 362P; 364W,F; 367A,K; 368K; 369I,L; 370V; 374S,A; 375H; 376M; 387P; 393V; 396R; 409R; 412R; 421F,Y; and 431E.   
     
     
         88 . The modified phytase of  claim 86 , wherein the phytase is an ascomycete phytase. 
     
     
         89 . The modified phytase of  claim 88 , wherein the phytase is an  Aspergillus  phytase. 
     
     
         90 . The modified phytase of  claim 89 , wherein the phytase is an  Aspergillus ficuum, Aspergillus fumigatus, Aspergillus nidulans, Aspergillus niger , or  Aspergillus terreus  phytase. 
     
     
         91 . The modified phytase of  claim 90 , wherein the phytase is an  Aspergillus terreus , CBS 116.46 phytase. 
     
     
         92 . The modified phytase of  claim 86 , wherein the phytase is a  Myceliophthora thermophila, Talaromyces thermophilus , or  Thermomyces lanuginosus  phytase. 
     
     
         93 . The modified phytase of  claim 92 , wherein the phytase is a  Myceliophthora thermophila , ATCC 34625 or ATCC 74340 phytase. 
     
     
         94 . The modified phytase of  claim 88 , wherein the phytase is a  Talaromyces thermophilus,  ATCC 20186 or ATCC 74338 phytase. 
     
     
         95 . The modified phytase of  claim 88 , wherein the phytase is a  Thermomyces lanuginosus , NRRL B21527 phytase. 
     
     
         96 . The modified phytase of  claim 86 , wherein the phytase is an ascomycete consensus phytase sequence. 
     
     
         97 . The modified phytase of  claim 86 , wherein the phytase is a basidiomycete phytase. 
     
     
         98 . The modified phytase of  claim 97 , wherein the phytase is an  Agrocybe pediades, Paxillus involutus, Peniophora lycii , or  Trametes pubescens  phytase. 
     
     
         99 . The modified phytase of  claim 98 , wherein the phytase is a  Paxillus involutus , CBS 100231 phytase. 
     
     
         100 . The modified phytase of  claim 99 , wherein the phytase is a  Paxillus involutus , CBS 100231 Phy-A2 phytase. 
     
     
         101 . The modified phytase of  claim 98 , wherein the phytase is a  Trametes  pubescens, CBS 100232 phytase. 
     
     
         102 . A feed or food comprising a modified phytase of  claim 86 . 
     
     
         103 . A composition comprising a modified phytase of  claim 86 . 
     
     
         104 . A process for reducing phytate levels in animal manure comprising feeding an animal with an effective amount of the feed of  claim 102 .