Recombinant virus vector for gene transfer into lymphoid cells

Abstract

A recombinant herpesvirus, a method for producing the recombinant herpesvirus, and a pharmaceutical composition comprising the recombinant herpesvirus, are provided with a method for producing a recombinant herpesvirus using a BAC vector sequence. In addition, a vector comprising a herpesvirus genomic gene and a BAC vector sequence, a cell comprising the vector, and a nucleic acid cassette comprising a fragment, which is capable of homologous recombination with a herpesvirus genome, and a BAC vector sequence, are provided.

1 . A recombinant herpesvirus for gene transfer into lymphoid cells. 
     
     
         2 - 16 . (canceled) 
     
     
         17 . A pharmaceutical composition comprising the virus of  claim 1 . 
     
     
         18 . The pharmaceutical composition of  claim 17 , wherein the composition is in the form of vaccine. 
     
     
         19 . A vector comprising a human herpesvirus essential gene and a BAC vector sequence. 
     
     
         20 - 42 . (canceled) 
     
     
         43 . A method to produce a recombinant herpesvirus, comprising:
 introducing a vector comprising a herpesvirus genome essential gene and BAC vector sequence into a mammalian host cell; and   culturing the mammalian host cell to produce a recombinant herpesvirus.   
     
     
         44 - 60 . (canceled) 
     
     
         61 . A virus produced by the method of  claim 43 . 
     
     
         62 . A pharmaceutical composition comprising the virus of  claim 61 . 
     
     
         63 - 100 . (canceled)