Process for producing cytotoxic lymphocyte

Abstract

The present invention relates to a method for preparing a cytotoxic lymphocyte characterized in that the method comprises the step of carrying out at least one of induction, maintenance and expansion of a cytotoxic lymphocyte in the presence of fibronectin, a fragment thereof or a mixture thereof.

1 . A cell culture medium comprising an anti-CD3 antibody and an effective ingredient selected from the group consisting of fibronectin, a fragment thereof and a mixture thereof. 
     
     
         2 . The medium according to  claim 1 , wherein said anti-CD3 antibody and/or said effective ingredient are immobilized to a solid phase. 
     
     
         3 . The medium according to  claim 2 , wherein the solid phase is a petri dish, a flask, a bag, beads, a membrane or a glass slide. 
     
     
         4 . The medium according to  claim 1 , wherein said fibronectin fragment comprises at least one domain selected from the group consisting of a VLA-4 binding domain, a VLA-5 binding domain and a heparin binding domain. 
     
     
         5 . The medium according to  claim 1 , further comprising IL-2. 
     
     
         6 . A method of cultivating a cell, wherein said method comprises incubating a cell in the medium according to  claim 1 .