Methods and materials relating to stem cell growth factor-like polypeptides and polynucleotides

Abstract

The invention provides novel polynucleotides and polypeptides encoded by such polynucleotides and mutants or variants thereof that correspond to a novel human secreted stem cell growth factor-like polypeptides. Other aspects of the invention include vectors containing processes for producing novel human secreted stem cell growth factor-like polypeptides, and antibodies specific for such polypeptides.

1 . An isolated polypeptide comprising an amino acid sequence selected from the group consisting of amino acids 21-263 of SEQ ID NO:18 and amino acids 21-263 of SEQ ID NO:27. 
     
     
         2 . The polypeptide of  claim 1 , comprising the amino acid sequence of amino acids 21-263 of SEQ ID NO:18. 
     
     
         3 . The polypeptide of  claim 1 , comprising the amino acid sequence of amino acids 21-263 of SEQ ID NO:27. 
     
     
         4 . The polypeptide of  claim 1 , consisting of the amino acid sequence of amino acids 21-263 of SEQ ID NO:18. 
     
     
         5 . The polypeptide of  claim 1 , consisting of the amino acid sequence of amino acids 21-263 of SEQ ID NO:27. 
     
     
         6 . A composition comprising the polypeptide of  claim 1  and a pharmaceutically acceptable excipient. 
     
     
         7 . A composition comprising the polypeptide of  claim 2  and a pharmaceutically acceptable excipient. 
     
     
         8 . A composition comprising the polypeptide of  claim 3  and a pharmaceutically acceptable excipient. 
     
     
         9 . A composition comprising the polypeptide of  claim 4  and a pharmaceutically acceptable excipient. 
     
     
         10 . A composition comprising the polypeptide of  claim 5  and a pharmaceutically acceptable excipient. 
     
     
         11 . A method of producing the polypeptide of  claim 1  comprising:
 (a) culturing a host cell comprising a polynucleotide sequence encoding said polypeptide, under conditions sufficient to express the polypeptide in said cell; and   (b) isolating the polypeptide from the cell culture or cells in step (a).   
     
     
         12 . A method of producing the polypeptide of  claim 2  comprising:
 (a) culturing a host cell comprising a polynucleotide sequence encoding said polypeptide, under conditions sufficient to express the polypeptide in said cell; and   (b) isolating the polypeptide from the cell culture or cells in step (a).   
     
     
         13 . A method of producing the polypeptide of  claim 3  comprising:
 (a) culturing a host cell comprising a polynucleotide sequence encoding said polypeptide, under conditions sufficient to express the polypeptide in said cell; and   (b) isolating the polypeptide from the cell culture or cells in step (a).   
     
     
         14 . A method of producing the polypeptide of  claim 4  comprising:
 (a) culturing a host cell comprising a polynucleotide sequence encoding said polypeptide, under conditions sufficient to express the polypeptide in said cell; and   (b) isolating the polypeptide from the cell culture or cells in step (a).   
     
     
         15 . A method of producing the polypeptide of  claim 5  comprising:
 (a) culturing a host cell comprising a polynucleotide sequence encoding said polypeptide, under conditions sufficient to express the polypeptide in said cell; and   (b) isolating the polypeptide from the cell culture or cells in step (a).   
     
     
         16 . A method of producing a composition comprising combining the polypeptide of  claim 1  with a pharmaceutically acceptable excipient. 
     
     
         17 . A method of producing a composition comprising combining the polypeptide of  claim 2  with a pharmaceutically acceptable excipient. 
     
     
         18 . A method of producing a composition comprising combining the polypeptide of  claim 3  with a pharmaceutically acceptable excipient. 
     
     
         19 . A method of producing a composition comprising combining the polypeptide of  claim 4  with a pharmaceutically acceptable excipient. 
     
     
         20 . A method of producing a composition comprising combining the polypeptide of  claim 5  with a pharmaceutically acceptable excipient.