Reagents for detecting efavirenz

Abstract

The invention provides derivatives of efavirenz and methods of making derivatives of efavirenz. The derivatives include immunogenic compounds for producing antibodies to efavirenz and labeled efavirenz tracers. These compounds are useful in immunoassay methods for determining efavirenz.

1 . An antibody produced in response to a compound having the structure 
       
         
           
           
               
               
           
         
         wherein L is NH or O; 
         R 1  is a saturated or unsaturated, substituted or unsubstituted, straight or branched chain of 0-10 carbon or hetero atoms; 
         X is a linker group consisting of 0-2 substituted or unsubstituted aromatic rings or aliphatic linking groups containing 0-10 carbon or hetero atoms; and 
         Y is NH-Z where Z is a carrier selected from the group consisting of poly(amino acid)s, polysaccharides, poly(nucleic acid)s, and particles. 
       
     
     
         2 . A monoclonal antibody specific for efavirenz characterized by having less than 1 percent cross-reactivity with one or more protease inhibitors selected from the group consisting of nelfinavir, saquinavir, indinavir, ritonavir, amprenavir, lopinavir, and atazanavir. 
     
     
         3 . A monoclonal antibody specific for efavirenz characterized by haying less than 1 percent cross-reactivity with one or more nucleoside reverse transcriptase inhibitors selected from the group consisting of 3′-azido-3′-deoxythymidine and 2′,3′-didehydro-3′-deoxythymidine. 
     
     
         4 . A monoclonal antibody specific for efavirenz characterized by having less than 1 percent cross-reactivity with one or more non-nucleoside reverse transcriptase inhibitors selected from the group consisting of nevirapine and delaviradine. 
     
     
         5 . A monoclonal antibody specific for efavirenz characterized by having less than 1 percent cross-reactivity with one or more protease inhibitors selected from the group consisting of nelfinavir, saquinavir, indinavir, ritonavir, amprenavir, lopinavir, and atazanavir. 
     
     
         6 . Cell line EFA 97.1, ATCC designation PTA-5820, producing monoclonal antibody having specificity for efavirenz. 
     
     
         7 . A monoclonal antibody produced from cell line EFA 97.1, ATCC designation PTA-5820, the antibody having specificity for efavirenz. 
     
     
         8 . A monoclonal antibody having specificity for efavirenz and binding in a manner equivalent to that of an antibody from cell line EFA 97.1, ATCC designation PTA-5820.