Method of producing tissue by placing a molding support within a body cavity

Abstract

A method of producing a tissue includes placing a molding support within a body cavity for a time and under conditions sufficient for non-vascularized tissue comprising myofibroblasts to form on the molding support. In some embodiments, the tissue produced by this method is particularly useful as vascular tissue for the treatment or prophylaxis of diseased or damaged blood vessels such as in atherosclerosis.

1 . A method of producing a tissue, comprising placing a molding support within a peritoneal cavity for a time and under conditions sufficient for non-vascularized tissue comprising myofibroblasts to form on the molding support and grafting the tissue into a recipient. 
     
     
         2 . The method of  claim 1 , further comprising removing the molding support from the peritoneal cavity, prior to grafting. 
     
     
         3 . The method of  claim 1  further comprising separating the tissue from the molding support. 
     
     
         4 . The method of  claim 3 , further comprising everting the tissue. 
     
     
         5 . The method of  claim 1 , wherein the molding support is placed into the peritoneal cavity by surgical implantation. 
     
     
         6 . The method of  claim 1 , wherein the tissue is covered by non-thrombogenic mesothelial cells. 
     
     
         7 . The method of  claim 1 , wherein the molding support comprises a biodegradable matrix. 
     
     
         8 . The method of  claim 1 , wherein the molding support is a foreign body. 
     
     
         9 . The method of  claim 8 , wherein the molding support comprises silicone. 
     
     
         10 . The method of  claim 1 , wherein the molding support is a tubular molding. 
     
     
         11 . The method of  claim 10 , wherein the tissue is vascular. 
     
     
         12 . The method of  claim 1 , wherein the tissue is blood vessel. 
     
     
         13 . The method of  claim 12 , wherein the blood vessel is selected from the group consisting of an artery, an arterio-venous shunt and an access fistula. 
     
     
         14 . The method of  claim 1 , wherein the tissue is suitable for use as a substitute blood vessel. 
     
     
         15 . The method of  claim 1 , wherein the tissue is autologous relative to the recipient. 
     
     
         16 . The method of  claim 1 , wherein the recipient is human. 
     
     
         17 . The method of  claim 1 , further comprising maintaining the non-vascularized tissue in a frozen state. 
     
     
         18 . The method of producing a tissue, comprising placing a molding support within a peritoneal cavity for a time and under conditions sufficient for non-vascularized tissue comprising myofibroblasts to form on the molding support and grafting the tissue into a recipient, wherein the molding support is a tube and wherein the method further comprises placing the tube into the peritoneal cavity using a prosthetic device that comprises:
 an outer elongated tubular member having perforations or a permeable layer to permit passage of cells and fluid into and out of the tubular member;   an inner elongated member removably insertable in the outer elongated member and comprising the tube around which or part of which vascular or non-vascular tissue can grow; and   an external portion which is maintained on the skin surface or subcutaneously and through which the inner elongated member can be removed from the outer elongated member.   
     
     
         19 . The method of  claim 18 , wherein the outer elongated tubular membrane is comprised of silicone. 
     
     
         20 . The method of  claim 18 , wherein the perforations are about 2 mm in diameter.