US2008274129A1PendingUtilityA1

Hla-A2 Tumor Associated Antigen Peptides and Compositions

52
Assignee: FIKES JOHN DPriority: Apr 18, 2003Filed: Apr 16, 2004Published: Nov 6, 2008
Est. expiryApr 18, 2023(expired)· nominal 20-yr term from priority
A61P 35/04A61P 35/00A61K 2039/55511A61K 2039/572C07K 7/06A61K 39/39A61K 2039/57A61K 2039/55566A61K 2039/70C07K 14/47A61K 40/4268A61K 40/4266A61K 40/4241A61K 40/4205A61K 40/24A61K 40/19A61K 2239/31A61K 39/0011
52
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Claims

Abstract

A peptide or composition comprising at least one HLA-A2 epitope or analog from CEA, HER2/neu, MAGE2, MAGE3, or p53.

Claims

exact text as granted — not AI-modified
1 - 14 . (canceled) 
     
     
         15 . A composition comprising at least three peptides, wherein each of said three peptides is less than 15 amino acid residues in length and comprises a cytotoxic T-cell lymphocyte (CTL) epitope and/or analog selected from the group consisting of: 
       
         
           
                 
                 
                 
                 
               
                     
                   RLLQETELV, 
                   (SEQ ID NO:2) 
                     
                 
                     
                     
                 
                     
                   YLQLVFGIEV, 
                   (SEQ ID NO:3) 
                 
                     
                     
                 
                     
                   LLTFWNPPV, 
                   (SEQ ID NO:4) 
                 
                     
                     
                 
                     
                   SMPPPGTRV, 
                   (SEQ ID NO:5) 
                 
                     
                     
                 
                     
                   KLBPVQLWV, 
                   (SEQ ID NO:6) 
                 
                     
                     
                 
                     
                   KVFGSLAFV, 
                   (SEQ ID NO:7) 
                 
                     
                     
                 
                     
                   YLSGADLNL, 
                   (SEQ ID NO:8) 
                 
                     
                     
                 
                     
                   IMIGHLVGV, 
                   (SEQ ID NO:9) 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   KVAEIVHFL. 
                   (SEQ ID NO:10) 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         16 . A composition according to  claim 15 , further comprising a fourth peptide, wherein said fourth peptide is less than 15 amino acid residues in length and comprises a cytotoxic T-cell lymphocyte (CTL) epitope and/or analog selected from the group consisting of: 
       
         
           
                 
                 
                 
                 
               
                     
                   RLLQETELV, 
                   (SEQ ID NO:2) 
                     
                 
                     
                     
                 
                     
                   YLQLVFGIEV, 
                   (SEQ ID NO:3) 
                 
                     
                     
                 
                     
                   LLTFWNPPV, 
                   (SEQ ID NO:4) 
                 
                     
                     
                 
                     
                   SMPPPGTRV, 
                   (SEQ ID NO:5) 
                 
                     
                     
                 
                     
                   KLBPVQLWV, 
                   (SEQ ID NO:6) 
                 
                     
                     
                 
                     
                   KVFGSLAFV, 
                   (SEQ ID NO:7) 
                 
                     
                     
                 
                     
                   YLSGADLNL, 
                   (SEQ ID NO:8) 
                 
                     
                     
                 
                     
                   IMIGHLVGV, 
                   (SEQ ID NO:9) 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   KVAEIVHFL. 
                   (SEQ ID NO:10) 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         17 . A composition according to  claim 16 , further comprising a fifth peptide, wherein said fifth peptide is less than 15 amino acid residues in length and comprises a cytotoxic T-cell lymphocyte (CTL) epitope and/or analog selected from the group consisting of: 
       
         
           
                 
                 
                 
                 
               
                     
                   RLLQETELV, 
                   (SEQ ID NO:2) 
                     
                 
                     
                     
                 
                     
                   YLQLVFGIEV, 
                   (SEQ ID NO:3) 
                 
                     
                     
                 
                     
                   LLTFWNPPV, 
                   (SEQ ID NO:4) 
                 
                     
                     
                 
                     
                   SMPPPGTRV, 
                   (SEQ ID NO:5) 
                 
                     
                     
                 
                     
                   KLBPVQLWV, 
                   (SEQ ID NO:6) 
                 
                     
                     
                 
                     
                   KVFGSLAFV, 
                   (SEQ ID NO:7) 
                 
                     
                     
                 
                     
                   YLSGADLNL, 
                   (SEQ ID NO:8) 
                 
                     
                     
                 
                     
                   IMIGHLVGV, 
                   (SEQ ID NO:9) 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   KVAEIVHFL. 
                   (SEQ ID NO:10) 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         18 . A composition according to  claim 17 , further comprising a sixth peptide, wherein said sixth peptide is less than 15 amino acid residues in length and comprises a cytotoxic T-cell lymphocyte (CTL) epitope and/or analog selected from the group consisting of: 
       
         
           
                 
                 
                 
                 
               
                     
                   RLLQETELV, 
                   (SEQ ID NO:2) 
                     
                 
                     
                     
                 
                     
                   YLQLVFGIEV, 
                   (SEQ ID NO:3) 
                 
                     
                     
                 
                     
                   LLTFWNPPV, 
                   (SEQ ID NO:4) 
                 
                     
                     
                 
                     
                   SMPPPGTRV, 
                   (SEQ ID NO:5) 
                 
                     
                     
                 
                     
                   KLBPVQLWV, 
                   (SEQ ID NO:6) 
                 
                     
                     
                 
                     
                   KVFGSLAFV, 
                   (SEQ ID NO:7) 
                 
                     
                     
                 
                     
                   YLSGADLNL, 
                   (SEQ ID NO:8) 
                 
                     
                     
                 
                     
                   IMIGHLVGV, 
                   (SEQ ID NO:9) 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   KVAEIVHFL. 
                   (SEQ ID NO:10) 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         19 . A composition according to  claim 18 , further comprising a seventh peptide, wherein said seventh peptide is less than 15 amino acid residues in length and comprises a cytotoxic T-cell lymphocyte (CTL) epitope and/or analog selected from the group consisting of: 
       
         
           
                 
                 
                 
                 
               
                     
                   RLLQETELV, 
                   (SEQ ID NO:2) 
                     
                 
                     
                     
                 
                     
                   YLQLVFGIEV, 
                   (SEQ ID NO:3) 
                 
                     
                     
                 
                     
                   LLTFWNPPV, 
                   (SEQ ID NO:4) 
                 
                     
                     
                 
                     
                   SMPPPGTRV, 
                   (SEQ ID NO:5) 
                 
                     
                     
                 
                     
                   KLBPVQLWV, 
                   (SEQ ID NO:6) 
                 
                     
                     
                 
                     
                   KVFGSLAFV, 
                   (SEQ ID NO:7) 
                 
                     
                     
                 
                     
                   YLSGADLNL, 
                   (SEQ ID NO:8) 
                 
                     
                     
                 
                     
                   IMIGHLVGV, 
                   (SEQ ID NO:9) 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   KVAEIVHFL. 
                   (SEQ ID NO:10) 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         20 . A composition according to  claim 19 , further comprising a eighth peptide, wherein said eighth peptide is less than 15 amino acid residues in length and comprises a cytotoxic T-cell lymphocyte (CTL) epitope and/or analog selected from the group consisting of: 
       
         
           
                 
                 
                 
                 
               
                     
                   RLLQETELV, 
                   (SEQ ID NO:2) 
                     
                 
                     
                     
                 
                     
                   YLQLVFGIEV, 
                   (SEQ ID NO:3) 
                 
                     
                     
                 
                     
                   LLTFWNPPV, 
                   (SEQ ID NO:4) 
                 
                     
                     
                 
                     
                   SMPPPGTRV, 
                   (SEQ ID NO:5) 
                 
                     
                     
                 
                     
                   KLBPVQLWV, 
                   (SEQ ID NO:6) 
                 
                     
                     
                 
                     
                   KVFGSLAFV, 
                   (SEQ ID NO:7) 
                 
                     
                     
                 
                     
                   YLSGADLNL, 
                   (SEQ ID NO:8) 
                 
                     
                     
                 
                     
                   IMIGHLVGV, 
                   (SEQ ID NO:9) 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   KVAEIVHFL. 
                   (SEQ ID NO:10) 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         21 . A composition according to  claim 20 , further comprising a ninth peptide, wherein said ninth peptide is less than 15 amino acid residues in length and comprises a cytotoxic T lymphocyte (CTL) epitope and/or analog selected from the group consisting of: 
       
         
           
                 
                 
                 
                 
               
                     
                   RLLQETELV, 
                   (SEQ ID NO:2) 
                     
                 
                     
                     
                 
                     
                   YLQLVFGIEV, 
                   (SEQ ID NO:3) 
                 
                     
                     
                 
                     
                   LLTFWNPPV, 
                   (SEQ ID NO:4) 
                 
                     
                     
                 
                     
                   SMPPPGTRV, 
                   (SEQ ID NO:5) 
                 
                     
                     
                 
                     
                   KLBPVQLWV, 
                   (SEQ ID NO:6) 
                 
                     
                     
                 
                     
                   KVFGSLAFV, 
                   (SEQ ID NO:7) 
                 
                     
                     
                 
                     
                   YLSGADLNL, 
                   (SEQ ID NO:8) 
                 
                     
                     
                 
                     
                   IMIGHLVGV, 
                   (SEQ ID NO:9) 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   KVAEIVHFL. 
                   (SEQ ID NO:10) 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         22 . A composition according to  claim 15 , further comprising an additional peptide, wherein said additional peptide is less than 25 amino acid residues in length and comprises an helper T lymphocyte (HTL) epitope. 
     
     
         23 . A composition according to  claim 22 , wherein said additional peptide is a PanDR binding peptide. 
     
     
         24 . A composition according to  claim 23 , wherein said Pan DR binding peptide comprises the amino acid sequence aKXVAAWTLKAAa (SEQ ID NO:1). 
     
     
         25 . A composition according to  claim 15 , further comprising a liposome. 
     
     
         26 . A composition according to  claim 15 , further comprising a lipid. 
     
     
         27 . A composition according to  claim 15 , further comprising an antigen presenting cell. 
     
     
         28 . A composition according to  claim 27 , wherein said antigen presenting cell is a dendritic cell. 
     
     
         29 . A composition according to  claim 15 , further comprising a pharmaceutical excipient. 
     
     
         30 . A composition according to  claim 29 , wherein said pharmaceutical excipient is an adjuvant. 
     
     
         31 . A composition according to  claim 30 , wherein said adjuvant is a mineral oil adjuvant. 
     
     
         32 . An isolated nucleic acid encoding at least three peptides, wherein each of said three peptides is less than 15 amino acid residues in length and comprises a cytotoxic T-cell lymphocyte (CTL) epitope and/or analog selected from the group consisting of: 
       
         
           
                 
                 
                 
                 
               
                     
                   RLLQETELV, 
                   (SEQ ID NO:2) 
                     
                 
                     
                     
                 
                     
                   YLQLVFGIEV, 
                   (SEQ ID NO:3) 
                 
                     
                     
                 
                     
                   LLTFWNPPV, 
                   (SEQ ID NO:4) 
                 
                     
                     
                 
                     
                   SMPPPGTRV, 
                   (SEQ ID NO:5) 
                 
                     
                     
                 
                     
                   KLBPVQLWV, 
                   (SEQ ID NO:6) 
                 
                     
                     
                 
                     
                   KVFGSLAFV, 
                   (SEQ ID NO:7) 
                 
                     
                     
                 
                     
                   YLSGADLNL, 
                   (SEQ ID NO:8) 
                 
                     
                     
                 
                     
                   IMIGHLVGV, 
                   (SEQ ID NO:9) 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   KVAEIVHFL. 
                   (SEQ ID NO:10) 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         33 . A method for the treatment or prevention of cancer, said method comprising administering the composition of  claim 15  to a patient in need thereof. 
     
     
         34 . A method according to  claim 33 , wherein said treatment delays the recurrence of cancer following surgery, radiation therapy or chemotherapy. 
     
     
         35 . A method according to  claim 33 , wherein said treatment prevents the metastasis of a primary tumor. 
     
     
         36 . A method according to  claim 33 , wherein said cancer is selected from the group consisting of breast cancer, colon cancer, lung cancer, non-small cell lung cancer, ovarian cancer, gastric cancer, melanoma and a cancer of the head and/or neck. 
     
     
         37 . A method for the treatment or prevention of cancer, said method comprising administering the nucleic acid of  claim 32  to a patient in need thereof. 
     
     
         38 . A method according to  claim 37 , wherein said treatment delays the recurrence of cancer following surgery, chemotherapy or radiation. 
     
     
         39 . A method according to  claim 37 , wherein said treatment prevents the metastasis of a primary tumor. 
     
     
         40 . A method according to  claim 37 , wherein said cancer is selected from the group consisting of breast cancer, colon cancer, lung cancer, non-small cell lung cancer, ovarian cancer, gastric cancer, melanoma and a cancer of the head and/or neck.

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