Body for flow-through cells and the use thereof

Abstract

A system of flow-through cells is obtained by assembling a body with a base plate. The body has a first spring element and a first stop, disposed on opposite end sections of the body, at least one second spring element and a corresponding second stop, disposed on opposite faces of the body, and at least one support element and at least one retaining element, which are adapted for the exact positioning of the body in the receiving openings of a support in three directions.

1 - 16 . (canceled) 
     
     
         17 . A body ( 11 ) for the formation of a linear row or of a matrix-like arrangement of flow-through cells by joining of body ( 11 ) with a base plate ( 31 ),
 this body ( 11 ) comprising the following elements, which serve to position the body precisely in the receiving opening of a carrier ( 41 ):   (a) a first spring element ( 51 ) and a first stop ( 52 ), which serve to position the body precisely in an initial direction (X-direction) and are arranged at opposite end sections of the body ( 11 ),   (b) at least one second spring element ( 53 ,  55 ) and matching second stop ( 54 ,  56 ), which serve to position the body ( 11 ) precisely in a second direction (Y-direction) and are arranged on opposite sides of the body ( 11 ), wherein the second direction (Y-direction) is perpendicular to the first direction (X-direction),   (c) at least one element of support ( 57 ,  58 ,  59 ) and at least one holding element ( 61 ,  62 ), wherein this element of support and holding element together serve to position the body precisely in a third direction (Z-direction), which lies perpendicular to a plane which is defined by two axes, namely by an axis in the first and another axis in the second direction.   
     
     
         18 . A body according to  claim 17 , comprising at least one and preferably all of the following features:
 (i) at least one other second spring element ( 55 ) and at least one other matching second stop ( 56 );   (ii) at least two further elements of support;   (iii) the elements of support are points of support ( 57 ,  58 ,  59 );   (iv) at least one other holding element;   (v) the holding elements ate snap-on hooks ( 61 ,  62 ).   
     
     
         19 . A body according to  claim 17 , comprising an outer form which fits into a receiving opening ( 42 ) of the carrier ( 41 ). 
     
     
         20 . A first arrangement of flow-through cells formed by joining together a body ( 11 ) according to  claim 19  and a base plate ( 31 ). 
     
     
         21 . A first arrangement of flow-through cells according to  claim 20 , wherein the base plate ( 31 ) carries biological and/or biochemical and/or synthetic recognition elements. 
     
     
         22 . A first arrangement of flow-through cells according to  claim 20 , wherein the base plate ( 31 ) is suitable for use as a waveguide. 
     
     
         23 . A carrier for accommodating ( 41 ) at least a first arrangement of flow-through cells according to  claim 20 , comprising at least one recess ( 42 ) whose form and dimensions are adapted to the outer form of the body ( 11 ) such that it allows a precise positioning of the body ( 11 ) in the recess ( 42 ). 
     
     
         24 . A carrier ( 41 ) according to  claim 23 , wherein said at least one recess comprises multiple recesses ( 42 ). 
     
     
         25 . A second arrangement of flow-through cells comprising a first arrangement of flow-through cells according to  claim 20  accommodated in a carrier comprising at least one recess ( 42 ) whose form and dimensions are adapted to the outer form of the body ( 11 ) such that it allows a precise positioning of the body ( 11 ) in the recess ( 42 ). 
     
     
         26 . A third arrangement of flow-through cells comprising
 a body ( 11 ) according to  claim 17 ;   a carrier ( 41 ) comprising at least one recess ( 42 ) whose form and dimensions are adapted to the outer form of the body ( 11 ) such that it allows a precise positioning of the body ( 11 ) in the recess ( 42 ); and   a base plate ( 31 ) inserted between the body ( 11 ) and the carrier ( 41 ).   
     
     
         27 . A third arrangement of flow-through cells according to  claim 26 , wherein the base plate ( 31 ) is joined together with the body ( 11 ) solely by means of a force which is generated by the combination of the body ( 11 ) and the carrier ( 41 ). 
     
     
         28 . A third arrangement of flow-through cells according to  claim 26  comprising sealing agents for fluidic sealing of the interior space ( 13 ). 
     
     
         29 . A method comprising determining one or more analytes in a sample liquid with a body according to  claim 17 . 
     
     
         30 . A method comprising performing at least one of:
 quantitative or qualitative analyses for the determination of chemical, biochemical or biological analytes in screening methods in pharmaceutical research, combinatorial chemistry, clinical and preclinical development,   real-time binding studies and for the determination of kinetic parameters in affinity screening and in research,   qualitative and quantitative analyte determinations,
 especially for DNA and RNA analysis and the determination of genomic and proteomic differences in the genome, such as single nucleotide polymorphisms, 
   measurement of DNA-protein interactions,   determination of control mechanisms for mRNA expression and for protein (bio)synthesis,   generation of toxicity studies,   determination of expression profiles,
 in particular for the determination of biological and chemical marker substances, such as mRNA, proteins, peptides or low-molecular organic (messenger) substances, 
   determination of antibodies, antigens, pathogens or bacteria in pharmaceutical product research and development, human and veterinary diagnostics, agrochemical product research and development, for symptomatic and presymptomatic plant diagnostics,   patient stratification in pharmaceutical product development and for therapeutic drug selection, and   determination of pathogens and noxious substances,
 in particular salmonellae, prions, viruses and bacteria, especially in food and environmental analysis, 
   with a body according to  claim 17 .   
     
     
         31 . A method comprising deforming one or more analytes in a sample liquid with a carrier according to  claim 23 . 
     
     
         32 . A method comprising performing at least one of:
 quantitative or qualitative analyses for the determination of chemical, biochemical or biological analytes in screening methods in pharmaceutical research, combinatorial chemistry, clinical and preclinical development,   real-time binding studies and for the determination of kinetic parameters in affinity screening and in research,   qualitative and quantitative analyte determinations,
 especially for DNA and RNA analysis and the determination of genomic and proteomic differences in the genome, such as single nucleotide polymorphisms, 
   measurement of DNA-protein interactions,   determination of control mechanisms for mRNA expression and for protein (bio)synthesis,   generation of toxicity studies,   determination of expression profiles,
 in particular for the determination of biological and chemical marker substances, such as mRNA, proteins, peptides or low-molecular organic (messenger) substances, 
   determination of antibodies, antigens, pathogens or bacteria in pharmaceutical product research and development, human and veterinary diagnostics, agrochemical product research and development, for symptomatic and presymptomatic plant diagnostics,   patient stratification in pharmaceutical product development and for therapeutic drug selection, and   determination of pathogens and noxious substances,
 in particular salmonellae, prions, viruses and bacteria, especially in food and environmental analysis, 
   with a carrier according to  claim 23 .   
     
     
         33 . A method comprising determining one or more analytes in a sample liquid with an arrangement of flow-though cells according to  claim 25 . 
     
     
         34 . A method comprising determining at least one of:
 quantitative or qualitative analyses for the determination of chemical, biochemical or biological analytes in screening methods in pharmaceutical research, combinatorial chemistry, clinical and preclinical development,   real-time binding studies and for the determination of kinetic parameters in affinity screening and in research,   qualitative and quantitative analyte determinations,
 especially for DNA and RNA analysis and the determination of genomic and proteomic differences in the genome, such as single nucleotide polymorphisms, 
   measurement of DNA-protein interactions,   determination of control mechanisms for mRNA expression and for protein (bio)synthesis,   generation of toxicity studies,   determination of expression profiles,
 in particular for the determination of biological and chemical marker substances, such as mRNA, proteins, peptides or low-molecular organic (messenger) substances, 
   determination of antibodies, antigens, pathogens or bacteria in pharmaceutical product research and development, human and veterinary diagnostics, agrochemical product research and development, for symptomatic and presymptomatic plant diagnostics,   patient stratification in pharmaceutical product development and for therapeutic drug selection, and   determination of pathogens and noxious substances,
 in particular salmonellae, prions, viruses and bacteria, especially in food and environmental analysis, 
   with an arrangement of flow-through cells according to  claim 25 .   
     
     
         35 . A second arrangement of flow-through cells according to  claim 25 , wherein said at least one recess comprises multiple recesses ( 42 ). 
     
     
         36 . A method comprising determining one or more analytes in a sample liquid with an arrangement of flow-through cells according to  claim 20 . 
     
     
         37 . A method comprising determining at least one of:
 quantitative or qualitative analyses for the determination of chemical, biochemical or biological analytes in screening methods in pharmaceutical research, combinatorial chemistry, clinical and preclinical development,   real-time binding studies and for the determination of kinetic parameters in affinity screening and in research,   qualitative and quantitative analyte determinations,
 especially for DNA and RNA analysis and the determination of genomic and proteomic differences in the genome, such as single nucleotide polymorphisms, 
   measurement of DNA-protein interactions,   determination of control mechanisms for mRNA expression and for protein (bio)synthesis,   generation of toxicity studies,   determination of expression profiles,
 in particular for the determination of biological and chemical marker substances, such as mRNA, proteins, peptides or low-molecular organic (messenger) substances, 
   determination of antibodies, antigens, pathogens or bacteria in pharmaceutical product research and development, human and veterinary diagnostics, agrochemical product research and development, for symptomatic and presymptomatic plant diagnostics,   patient stratification in pharmaceutical product development and for therapeutic drug selection, and   determination of pathogens and noxious substances,
 in particular salmonellae, prions, viruses and bacteria, especially in food and environmental analysis, 
   with an arrangement of flow-through cells according to  claim 20 .   
     
     
         38 . A third arrangement of flow-through cells according to  claim 26 , wherein said at least one recess comprises multiple recesses ( 42 ). 
     
     
         39 . A method comprising determining one or more analytes in a sample liquid with an arrangement of flow-through cells according to  claim 26 . 
     
     
         40 . A method comprising performing at least one of:
 quantitative or qualitative analyses for the determination of chemical, biochemical or biological analytes in screening methods in pharmaceutical research, combinatorial chemistry, clinical and preclinical development,   real-time binding studies and for the determination of kinetic parameters in affinity screening and in research,   qualitative and quantitative analyte determinations,
 especially for DNA and RNA analysis and the determination of genomic and proteomic differences in the genome, such as single nucleotide polymorphisms, 
   measurement of DNA-protein interactions,   determination of control mechanisms for mRNA expression and for protein (bio)synthesis,   generation of toxicity studies,   determination of expression profiles,
 in particular for the determination of biological and chemical marker substances, such as mRNA, proteins, peptides or low-molecular organic (messenger) substances, 
   determination of antibodies, antigens, pathogens or bacteria in pharmaceutical product research and development, human and veterinary diagnostics, agrochemical product research and development, for symptomatic and presymptomatic plant diagnostics,   patient stratification in pharmaceutical product development and for therapeutic drug selection, and   determination of pathogens and noxious substances,
 in particular salmonellae, prions, viruses and bacteria, especially in food and environmental analysis, 
   with an arrangement of flow-through cells according to  claim 26 .