Laminate sheet articles for tissue regeneration

Abstract

The invention is to articles of extracellular matrix. The articles comprise one or more sheets of mammalian extracellular matrix laminated together. A single sheet can be folded over and laminated on 3 sides. Two or more sheets can be laminated to each other at their edges. The sheets can further encase a composition comprising a cell or cells, such as for example, a stem cell. A single sheet can be folded over to encase a composition, or rolled to encase a composition with lamination at either end of the roll, for example. The invention also includes methods of using these articles to regenerate tissue at tissue defects, or heal wounds in damaged tissue.

1 . An article for wound healing and tissue regeneration comprising two or more sheets of mammalian extracellular matrix, said sheets of mammalian extracellular matrix laminated to each other to form a planar laminated article of mammalian extracellular matrix. 
     
     
         2 . The article of  claim 1 , wherein said planar laminated article comprises two sheets of mammalian extracellular matrix. 
     
     
         3 . The article of  claim 1 , wherein said two or more sheets of mammalian extracellular matrix are from different mammalian tissue sources. 
     
     
         4 . The article of  claim 3 , wherein a first sheet is from small Intestine submucosa (SIS) of a mammal, and a second sheet is from stomach submucosa (SS) of a mammal. 
     
     
         5 . The article of  claim 1 , wherein said lamination is complete. 
     
     
         6 . The article of  claim 1 , wherein said lamination is partial. 
     
     
         7 . The article of  claim 1 , wherein said sheets are rectangular, and said planar laminated article is rectangular. 
     
     
         8 . The article of  claim 1 , wherein said sheets are triangular, and said planar laminated article is triangular. 
     
     
         9 . The article of  claim 1 , wherein said sheets are circular, and said planar laminated article is circular. 
     
     
         10 . An article for wound healing and tissue regeneration comprising two or more sheets of mammalian extracellular matrix, said sheets of mammalian extracellular matrix laminated to each other to form a planar laminated article of mammalian extracellular matrix, said article further comprising in between at least two of said sheets at least one cell to further effect wound healing or tissue regeneration upon placement of said laminated article in a mammal at a site in said mammal in need of wound healing or tissue regeneration. 
     
     
         11 . The article of  claim 10 , wherein said cell is a stem cell. 
     
     
         12 . The article of  claim 10 , wherein said cell is selected from the group consisting of human embryonic stem cell, a fetal cardiomyocyte, a myofibroblast, a mesenchymal stem cell, an autotransplanted expanded cardiomyocyte, an adipocyte, a totipotent cell, a pluripotent cell, a blood stem cell, a myoblast, an adult stem cell, a bone marrow cell, a mesenchymal cell, an embryonic stem cell, a parenchymal cell, an epithelial cell, an endothelial cell, a mesothelial cell, a fibroblast, a myofibroblast, an osteoblast, a chondrocyte, an exogenous cell, an endogenous cell, a stem cell, a hematopoetic stem cell, a pluripotent stem cell, a bone marrow-derived progenitor cell, a progenitor cell, a myocardial cell, a skeletal cell, a fetal cell, an embryonic cell, an undifferentiated cell, a multi-potent progenitor cell, a unipotent progenitor cell, a monocyte, a cardiomyocyte, a cardiac myoblast, a skeletal myoblast, a macrophage, a capillary endothelial cell, a xenogenic cell, an allogenic cell, an adult stem cell, and a post-natal stem cell. 
     
     
         13 . The article of  claim 10 , wherein said cell is a mesechymal cell. 
     
     
         14 . A method comprising:
 a. identifying a defect or wound in mammalian tissue which could benefit from tissue regeneration or wound healing,   b. providing an article comprising two or more sheets of mammalian extracellular matrix, said sheets of mammalian extracellular matrix laminated to each other to form a planar laminated article of mammalian extracellular matrix,   c. contacting said defect or wound with said article, and   d. regenerating tissue at said defect or healing said wound thereby.   
     
     
         15 . The method of  claim 14 , wherein said defect or wound is in myocardial tissue. 
     
     
         16 . The method of  claim 14 , wherein said defect or wound is in pancreatic tissue. 
     
     
         17 . The method of  claim 14 , wherein said defect or wound is in liver tissue. 
     
     
         18 . The method of  claim 14 , wherein said article further comprises a composition comprising a cell between at least two sheets of extracellular matrix. 
     
     
         19 . The method of  claim 18 , wherein said cell is a stem cell. 
     
     
         20 . The method of  claim 18 , wherein said cell is selected from the group consisting of human embryonic stem cell, a fetal cardiomyocyte, a myofibroblast, a mesenchymal stem cell, an autotransplanted expanded cardiomyocyte, an adipocyte, a totipotent cell, a pluripotent cell, a blood stem cell, a myoblast, an adult stem cell, a bone marrow cell, a mesenchymal cell, an embryonic stem cell, a parenchymal cell, an epithelial cell, an endothelial cell, a mesothelial cell, a fibroblast, a myofibroblast, an osteoblast, a chondrocyte, an exogenous cell, an endogenous cell, a stem cell, a hematopoetic stem cell, a pluripotent stem cell, a bone marrow-derived progenitor cell, a progenitor cell, a myocardial cell, a skeletal cell, a fetal cell, an embryonic cell, an undifferentiated cell, a multi-potent progenitor cell, a unipotent progenitor cell, a monocyte, a cardiomyocyte, a cardiac myoblast, a skeletal myoblast, a macrophage, a capillary endothelial cell, a xenogenic cell, an allogenic cell, an adult stem cell, and a post-natal stem cell.