Use of the repressor glxR for the synthesis of lysine in corynebacterium glutamicum

Abstract

Isolated polypeptide sequence having the sequence of SEQ ID NO:1 or muteins thereof having the ability to bind cAMP and repress the expression of the aceB gene of C. glutamicum and which can be obtained from SEQ ID NO:1 by inserting, deleting or substituting up to 20% of the amino acids.

1 . Isolated polypeptide sequence having the sequence of SEQ ID NO:1 or muteins thereof having the ability to bind cAMP and repress the expression of the aceB gene of  C. glutamicum  and which can be obtained from SEQ ID NO:1 by inserting, deleting or substituting up to 20% of the amino acids. 
     
     
         2 . Isolated polypeptide sequence according to  claim 1  which possesses a cAMP binding domain and helix-turn-helix DNA binding motif. 
     
     
         3 . Isolated polynucleotide sequence coding for a polypeptide sequence according to  claims 1  to  2 . 
     
     
         4 . Use of a polynucleotide sequence according to  claim 3  for modulating at least the expression of the aceB of  Corynebacteria.    
     
     
         5 . Use according to  claim 4  in order to influence the biosynthesis of amino acids. 
     
     
         6 . Use according to  claim 5  in order to increase the biosynthesis of amino acids. 
     
     
         7 . Use according to  claim 6  wherein the amino acid is lysine. 
     
     
         8 . Process of producing lysine in a host of genus  Corynebacterium  by influencing the gene expression of at least the aceB gene by expressing a polynucleotide sequence according to  claim 3  in said host in a way which is different from the naturally occurring genome organization of  Corynebacterium.