US2008286863A1PendingUtilityA1

Method and solutions for cryopreserving oocytes, especially fresh human oocytes

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Assignee: FABBRI RAFFAELLAPriority: Mar 20, 2000Filed: Jun 26, 2008Published: Nov 20, 2008
Est. expiryMar 20, 2020(expired)· nominal 20-yr term from priority
Inventors:Raffaella Fabbri
A01N 1/10A01N 1/125
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Claims

Abstract

In a method for cryopreserving fresh human oocytes, freezing and thawing solutions are used, which solutions include 1,2 propanediol (PROH) and sucrose at a concentration of at least 0.3 M. The oocytes are exposed for 13 - 15 minutes to a solution including 1.5 M PROH and 0.3 M sucrose before starting the freezing process.

Claims

exact text as granted — not AI-modified
1 . Use of a solution for cryopreserving human oocytes, characterized in that said solution comprises a permeating cryoprotectant and sucrose at a concentration of at least 0.3M. 
   
   
       2 . Use of a solution according to  claim 1 , further characterized in that the permeating cryoprotectant comprises 1,2-propanediol (PROH) at a concentration of 1.5M, and wherein the solution is useable as a loading solution in cryopreserving processes of oocytes. 
   
   
       3 . Use of a solution according to  claim 1 , further characterized in that the permeating cryoprotectant comprises 1,2-propanediol (PROH) at a concentration of 1.0M, and wherein the solution is useable as a thawing solution in cryopreserving processes of oocytes. 
   
   
       4 . Use of a solution according to  claim 1 , further characterized in that the permeating cryoprotectant comprises 1,2-propanediol (PROH) at a concentration of 0.5M, and wherein the solution is useable as a thawing solution in cryopreserving processes of oocytes. 
   
   
       5 . Use of a solution according to  claim 1 , further characterized in that the solution is used as a thawing solution in cryopreserving processes for oocytes. 
   
   
       6 . Use of a solution according to  claim 1 , wherein the oocytes are fresh human oocytes. 
   
   
       7 . Use of a solution according to  claim 2 , wherein the oocytes are fresh human oocytes. 
   
   
       8 . Use of a solution according to  claim 3 , wherein the oocytes are fresh human oocytes. 
   
   
       9 . Use of a solution according to  claim 4 , wherein the oocytes are fresh human oocytes. 
   
   
       10 . Use of a solution according to  claim 5 , wherein the oocytes are fresh human oocytes.

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