Soybean promoters SC194 and flower-preferred expression thereof in transgenic plants

Abstract

The promoters of a soybean SC194 polypeptide and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants are described.

1 . An isolated polynucleotide comprising:
 a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1 or a full-length complement thereof; or   b) a nucleotide sequence comprising a sequence having at least 90% sequence identity, based on the BLASTN method of alignment, when compared to the sequence set forth in SEQ ID NO:1;   
       wherein said nucleotide sequence is a promoter. 
     
     
         2 . The isolated polynucleotide of  claim 1 , wherein the nucleotide sequence of b) has at 95% identity, based on the BLASTN method of alignment, when compared to the sequence set forth in SEQ ID NO:1. 
     
     
         3 . A recombinant DNA construct comprising the isolated polynucleotide of  claim 1  operably linked to at least one heterologous sequence. 
     
     
         4 . The recombinant DNA construct of  claim 3 , wherein the heterologous nucleotide sequence encodes a gene involved in anthocyanin biosynthesis, a gene involved in the synthesis of fragrant fatty acid derivatives, a gene that is determinative of flower morphology, or a gene involved in biosynthesis of plant cytokinin. 
     
     
         5 . The recombinant DNA construct of  claim 4 , wherein the gene involved in anthocyanin biosynthesis is dyhydroflavonol 4-reductase, flavonoid 3,5-hydroxylase, chalcone synthase, chalcone isomerase, flavonoid 3-hydroxylase, anthocyanin synthase, or UDP-glucose 3-O-flavonoid glucosyl transferase. 
     
     
         6 . The recombinant DNA construct of  claim 4 , wherein the gene involved in the synthesis of fragrant fatty acid derivatives is S-linalool synthase, acetyl CoA:benzylalcohol acetyltransferase, benzyl CoA:benzylalcohol benzoyl transferase, S-adenosyl-L-methionine:benzoic acid carboxylmethyl transferase, mycrene synthase, (E)-β-ocimene synthase, orcinol O-methyltransferase, or limonene synthase. 
     
     
         7 . The recombinant DNA construct of  claim 4 , wherein the gene that is determinative of flower morphology is AGAMOUS, APETALA, or PISTILLATA. 
     
     
         8 . The recombinant DNA construct of  claim 4 , wherein the gene involved in biosynthesis of plant cytokinin is isopentenyl transferase. 
     
     
         9 . A vector comprising the recombinant DNA construct of  claim 3 . 
     
     
         10 . A cell comprising the recombinant DNA construct of  claim 3 . 
     
     
         11 . The cell of  claim 10 , wherein the cell is a plant cell. 
     
     
         12 . A transgenic plant having stably incorporated into its genome the recombinant DNA construct of  claim 3 . 
     
     
         13 . The transgenic plant of  claim 12 , wherein the plant is a flowering plant. 
     
     
         14 . The transgenic plant of  claim 13 , wherein the flowering plant is rose, carnation, Gerbera, Chrysanthemum, tulip, Gladioli, Alstroemeria, Anthurium, Iisianthus, larkspur, irises, orchid, snapdragon, African violet, or azalea. 
     
     
         15 . A transgenic seed produced by the transgenic plant of  claim 12 . 
     
     
         16 . A method of expressing a coding sequence or a functional RNA in a flowering plant comprising:
 a) introducing the recombinant DNA construct of  claim 3  into the plant, wherein the at least one heterologous sequence comprises a coding sequence or a functional RNA;   b) growing the plant of step a); and   c) selecting a plant displaying expression of the coding sequence or the functional RNA of the recombinant DNA construct.   
     
     
         17 . A method of transgenically altering a marketable flower trait of a flowering plant, comprising:
 a) introducing a recombinant DNA construct of  claim 3  into the flowering plant;   b) growing a fertile, mature flowering plant resulting from step a); and   c) selecting a flowering plant expressing the at least one heterologous nucleotide sequence in flower tissue based on the altered marketable flower trait.   
     
     
         18 . The method of  claim 17  wherein the marketable flower trait is color, morphology, or fragrance. 
     
     
         19 . An isolated polynucleotide comprising:
 (a) a nucleotide sequence comprising a fragment of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, or SEQ ID NO:7, or a full-length complement thereof; or   (b) a nucleotide sequence comprising a sequence having at least 90% sequence identity, based on the BLASTN method of alignment, when compared to the nucleotide sequence of (a);   
       wherein said nucleotide sequence is a promoter. 
     
     
         20 . The isolated polynucleotide of  claim 19 , wherein the nucleotide sequence of (a) comprises SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, or SEQ ID NO:7. 
     
     
         21 . An isolated polynucleotide comprising:
 (a) a nucleotide sequence encoding a polypeptide having at least 90% sequence identity, based on the Clustal method of alignment, when compared to the sequence set forth in SEQ ID NO:20, or   (b) a full-length complement of the nucleotide sequence of (a).   
     
     
         22 . The isolated polynucleotide of  claim 21 , wherein the polypeptide has at least 95% sequence identity, based on the Clustal method of alignment, when compared to the sequence set forth in SEQ ID NO:20. 
     
     
         23 . The isolated polynucleotide of  claim 22  encoding the sequence set forth in SEQ ID NO:20. 
     
     
         24 . The isolated polynucleotide of  claim 23 , wherein the nucleotide sequence comprises the sequence set forth in SEQ ID NO:19. 
     
     
         25 . A vector comprising the isolated polynucleotide of  claim 21 . 
     
     
         26 . A recombinant DNA construct comprising the isolated polynucleotide of  claim 21  operably linked to a regulatory sequence. 
     
     
         27 . A cell comprising the recombinant DNA construct of  claim 26 . 
     
     
         28 . A plant comprising the recombinant DNA construct of  claim 26 . 
     
     
         29 . A seed comprising the recombinant DNA construct of  claim 26 . 
     
     
         30 . A method for transforming a cell, comprising transforming a cell with the isolated polynucleotide of  claim 21 . 
     
     
         31 . A method for producing a plant comprising transforming a plant cell with the isolated polynucleotide of  claim 21  and regenerating a plant from the transformed plant cell. 
     
     
         32 . An isolated polypeptide having at least 90% sequence identity, based on the Clustal method of alignment, when compared to the sequence set forth in SEQ ID NO:20. 
     
     
         33 . The isolated polypeptide of  claim 32 , wherein the isolated polypeptide has at least 95% sequence identity, based on the Clustal method of alignment, when compared to the sequence set forth in SEQ ID NO:20. 
     
     
         34 . The isolated polypeptide of  claim 33 , wherein the isolated polypeptide comprises the amino acid sequence set forth in SEQ ID NO:20.