Alkylguanyltransferase Assays

Abstract

This invention provides a method to determine alkylguanyltransferase activity in a sample, comprising steps of placing the sample in an appropriate condition so that the AGT is functional; contacting the sample with an AGT Detector under conditions permitting the binding of AGT and AGTD to produce a signal; and measuring the signal, thereby determining the AGT activity in said sample. This invention provides different uses of this method.

1 . A method to determine AGT activity, comprising AGT and an AGT binder carrying a signaling molecule which is transferred to AGT.  
   
   
       2 . The method according to  claim 1  in which the AGT binder is an inhibitor of AGT.  
   
   
       3 . The method according to  claim 1  in which the AGT binder is an activator of AGT.  
   
   
       4 . The method according to  claim 1  in which the AGT binder is EX.  
   
   
       5 . The method according to  claim 1  in which the AGT binder is an oligonucleotide containing EX.  
   
   
       6 . The method according to  claim 1  in which the signal molecule is a fluorophore.  
   
   
       7 . The method according to  claim 1  in which the signal molecule is biotin.  
   
   
       8 . A method to determine AGT activity, comprising: a) AGT, b) an AGT binder carrying a radioactive molecule which is transferred to AGT, and SPA bead that binds AGT directly or indirectly.  
   
   
       9 . The method according to  claim 8  in which the AGT binder is radioactive-labeled EX.  
   
   
       10 . The method according to  claim 1 , set forth in  FIG. 1 .  
   
   
       11 . A method to determine AGT activity, comprising of AGT and an AGT binder carrying a chemical moiety which is transferred to AGT and the chemical moiety transferred to AGT is detected by streptavidin.  
   
   
       12 . The Scintillation Proximity Assay for AGT, set forth in  FIG. 5 .  
   
   
       13 . An AGT binder, not previously known.  
   
   
       14 . An AGT binder, set forth in  FIG. 3  or  4 .  
   
   
       15 . A compound which binds to AGT, comprises an AGT binder and biotin.  
   
   
       16 . The compound according to  claim 15  wherein the AGT binder is EX.  
   
   
       17 . A composition comprising the compound of  claim 15  or  16 .