Carrier, process for producing same, bioreactor, and chip for surface plasmon resonance analysis

Abstract

A carrier comprises a base plate, a polymeric film, which has been bound on a surface of the base plate, and a ligand, which has been bound with the polymeric film. The ligand has been bound with the polymeric film at a density falling within the range of 1.0×10 16 pieces/mm 3 to 3.3×10 18 pieces/mm 3 . The carrier is produced with a process, comprising the steps of: causing the polymeric film to bind on the base plate, and causing the ligand to bind with the polymeric film, the step of causing the ligand to bind with the polymeric film being performed in an organic solvent.

1 . A carrier, comprising:
 i) a base plate,   ii) a polymeric film, which has been bound on a surface of the base plate, and   iii) a ligand, which has been bound with the polymeric film,   the ligand having been bound with the polymeric film at a density falling within the range of 1.0×10 16  pieces/mm 3  to 3.3×10 18  pieces/mm 3 .   
   
   
       2 . A carrier as defined in  claim 1  wherein the ligand is a nitrilotriacetic acid derivative. 
   
   
       3 . A carrier as defined in  claim 2  wherein a metal ion has been fixed to the ligand. 
   
   
       4 . A carrier as defined in  claim 3  wherein a physiologically active substance has been fixed to the metal ion. 
   
   
       5 . A carrier as defined in  claim 4  wherein the metal ion is a transition metal ion. 
   
   
       6 . A carrier as defined in  claim 5  wherein the physiologically active substance has a functional group, which is capable of coordinating with the transition metal ion, and
 the physiologically active substance has been fixed by the functional group to the transition metal ion.   
   
   
       7 . A carrier as defined in  claim 6  wherein the functional group is an imidazole group. 
   
   
       8 . A carrier as defined in  claim 7  wherein the polymeric film has been bound on the surface of the base plate via a self-assembled monolayer. 
   
   
       9 . A carrier as defined in  claim 8  wherein the polymeric film is a hydrophilic polymer. 
   
   
       10 . A process for producing a carrier as defined in  claim 1 , comprising the steps of:
 i) causing the polymeric film to bind on the base plate, and   ii) causing the ligand to bind with the polymeric film,   the step of causing the ligand to bind with the polymeric film being performed in an organic solvent.   
   
   
       11 . A process for producing a carrier as defined in  claim 10  wherein the organic solvent is an aprotic type polar solvent. 
   
   
       12 . A process for producing a carrier as defined in  claim 11  wherein the aprotic type polar solvent is selected from the group consisting of dimethyl sulfoxide and N,N-dimethylformamide. 
   
   
       13 . A bioreactor, comprising a carrier as defined in  claim 1 . 
   
   
       14 . A chip for surface plasmon resonance analysis, comprising:
 i) a sensor chip base plate,   ii) a metal film, which has been located on a surface of the sensor chip base plate,   iii) a water-soluble polymeric film, which has been bound with the metal film,   iv) a ligand, which has been bound with the water-soluble polymeric film,   v) a metal ion, which has coordinated with the ligand, and   vi) a physiologically active substance, which has been fixed to the metal ion,   the ligand having been bound with the water-soluble polymeric film at a density falling within the range of 1.0×10 16  pieces/mm 3  to 3.3×10 18  pieces/mm 3 ,   the physiologically active substance having at least seven histidine units, each of which is capable of coordinating with the metal ion,   the physiologically active substance having been fixed to the metal ion via each of the histidine units.   
   
   
       15 . A chip for surface plasmon resonance analysis as defined in  claim 14  wherein the physiologically active substance has at least eight histidine units. 
   
   
       16 . A chip for surface plasmon resonance analysis as defined in  claim 15  wherein the ligand is nitrilotriacetic acid. 
   
   
       17 . A chip for surface plasmon resonance analysis as defined in  claim 16  wherein the water-soluble polymeric film is constituted of carboxy-modified dextran. 
   
   
       18 . A chip for surface plasmon resonance analysis as defined in  claim 17  wherein the metal film is constituted of at least one kind of metal selected from the group consisting of gold, silver, copper, platinum, palladium, and aluminum.