Nucleic acid and amino acid sequences relating to streptococcus pneumoniae for diagnostics and therapeutics

Abstract

The invention provides isolated polypeptide and nucleic acid sequences derived from Streptococcus pneumoniae that are useful in diagnosis and therapy of pathological conditions; antibodies against the polypeptides; and methods for the production of the polypeptides. The invention also provides methods for the detection, prevention and treatment of pathological conditions resulting from bacterial infection.

1 . An isolated nucleic acid encoding a  S. pneumoniae  surface protein having at least 80% identity to SEQ ID NO: 3110. 
     
     
         2 . A recombinant expression vector comprising the nucleic acid of  claim 1  operably linked to a transcription regulatory element. 
     
     
         3 . A cell comprising the recombinant expression vector of  claim 2 . 
     
     
         4 . The isolated nucleic acid of  claim 1 , wherein the  S. pneumoniae  surface protein elicits an immune response. 
     
     
         5 . An isolated nucleic acid encoding a  S. pneumoniae  surface protein having at least 80% identity to SEQ ID NO: 5328. 
     
     
         6 . A recombinant expression vector comprising the nucleic acid of  claim 5  operably linked to a transcription regulatory element. 
     
     
         7 . A cell comprising the recombinant expression vector of  claim 6 . 
     
     
         8 . The isolated nucleic acid of  claim 5 , wherein the  S. pneumoniae  surface protein provides protective immunity against an infection by  S. pneumoniae.    
     
     
         9 . An isolated nucleic acid having at least 80% identity to SEQ ID NO: 449, wherein the isolated nucleic acid encodes a  S. pneumoniae  surface protein. 
     
     
         10 . A recombinant expression vector comprising the nucleic acid of  claim 9  operably linked to a transcription regulatory element. 
     
     
         11 . A cell comprising the recombinant expression vector of  claim 10 . 
     
     
         12 . The isolated nucleic acid of  claim 9 , wherein the  S. pneumoniae  surface protein elicits an immune response. 
     
     
         13 . An isolated nucleic acid having at least 80% identity to SEQ ID NO: 5327, wherein the isolated nucleic acid encodes a  S. pneumoniae  surface protein. 
     
     
         14 . A recombinant expression vector comprising the nucleic acid of  claim 13  operably linked to a transcription regulatory element. 
     
     
         15 . A cell comprising the recombinant expression vector of  claim 14 . 
     
     
         16 . The isolated nucleic acid of  claim 13 , wherein the  S. pneumoniae  surface protein elicits an immune response. 
     
     
         17 . An isolated nucleic acid selected from the group consisting of:
 a) a nucleic acid encoding a protein having at least 80% identity to SEQ ID NO: 5328;   b) a nucleic acid fully complementary to a nucleic acid encoding a protein having at least 80% identity to SEQ ID NO: 5328; and   c) an RNA of a) or b), wherein U is substituted for T;   wherein the isolated nucleic acid encodes a  S. pneumoniae  surface protein.   
     
     
         18 . A recombinant expression vector comprising the nucleic acid of  claim 17  operably linked to a transcription regulatory element. 
     
     
         19 . A cell comprising the recombinant expression vector of  claim 18 . 
     
     
         20 . The isolated nucleic acid of  claim 17 , wherein the  S. pneumoniae  surface protein provides protective immunity against an infection by  S. pneumoniae.    
     
     
         21 . An isolated polypeptide having at least 80% identity to SEQ ID No: 5328 wherein the polypeptide elicits an immune response. 
     
     
         22 . An isolated polypeptide comprising at least 20 contiguous amino acids of the amino acid sequence set forth in SEQ ID No: 5328, wherein the polypeptide elicits an immune response. 
     
     
         23 . An isolated nucleic acid molecule encoding a  S. pneumoniae  surface antigen comprising at least 20 contiguous amino acids of the amino acid sequence set forth in SEQ ID No: 5328. 
     
     
         24 . A recombinant expression vector comprising the nucleic acid of  claim 23  operably linked to a transcription regulatory element. 
     
     
         25 . A cell comprising the recombinant expression vector of  claim 24 . 
     
     
         26 . The isolated nucleic acid of  claim 23 , wherein the  S. pneumoniae  surface antigen elicits an immune response. 
     
     
         27 . A method of treating a subject, comprising the step of administering to the subject a composition that includes a  S. pneumoniae  surface protein having at least 80% identity to SEQ ID NO: 5328, wherein administration of the protein elicits an immune response to  S. pneumoniae.    
     
     
         28 . An isolated polypeptide having at least 80% identity to SEQ ID No: 3110 wherein the polypeptide elicits an immune response. 
     
     
         29 . An isolated polypeptide comprising at least 20 contiguous amino acids of the amino acid sequence set forth in SEQ ID No: 3110, wherein the polypeptide elicits an immune response. 
     
     
         30 . An isolated nucleic acid molecule encoding a  S. pneumoniae  surface antigen comprising at least 20 contiguous amino acids of the amino acid sequence set forth in SEQ ID No: 3110. 
     
     
         31 . A recombinant expression vector comprising the nucleic acid of  claim 30  operably linked to a transcription regulatory element. 
     
     
         32 . A cell comprising the recombinant expression vector of  claim 31 . 
     
     
         33 . The isolated nucleic acid of  claim 30 , wherein the  S. pneumoniae  surface antigen elicits an immune response. 
     
     
         34 . A method of treating a subject, comprising the step of administering to the subject a composition that includes a  S. pneumoniae  surface protein having at least 80% identity to SEQ ID NO: 3110, wherein administration of the protein elicits an immune response to  S. pneumoniae.