US2009026122A1PendingUtilityA1

Biocompatible solid-phase microextraction coatings and methods for their preparation

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Assignee: JANUSZPriority: Mar 11, 2002Filed: Jul 16, 2008Published: Jan 29, 2009
Est. expiryMar 11, 2022(expired)· nominal 20-yr term from priority
Inventors:Janusz B. PawliszynFlorin Marcel MusteataMihaela L. MusteataRobert E. ShireyLeonard M. SidiskyYong Chen
Y10T428/2933G01N 2030/062G01N 2030/009A61B 10/0045G01N 1/405B01J 20/283H01J 49/165A61B 5/150358
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Claims

Abstract

A biocompatible coating for solid phase microextraction (SPME) of a small molecule from a biological matrix. The coating comprises SPME particles and a biocompatible polymer. The biocompatible polymer (e.g. polyacrylonitrile) reduces adsorption of proteins or macromolecules onto the SPME particles and allows the SPME particles to extract the small molecule from the matrix. A process for coating a flexible fiber with a biocompatible coating. The process comprises: coating the fiber with a suspension of SPME particles, the SPME particles being suspended in a solution of a biocompatible polymer and a solvent, the biocompatible polymer can comprise polyacrylonitrile (PAN); drying the coated fiber to remove the solvent; and curing the dried coated fiber at an elevated temperature.

Claims

exact text as granted — not AI-modified
1 . A biocompatible coating for solid phase microextraction (SPME) of a small molecule from a matrix, the coating comprising:
 SPME particles; and   a biocompatible polymer;   wherein the biocompatible polymer reduces adsorption of proteins or macromolecules onto the SPME particles and allows the SPME particles to extract the small molecule from the matrix.   
   
   
       2 . The biocompatible coating according to  claim 1 , wherein the SPME particles are selected from the group consisting of C-18/silica particles, RP-amide/silica particles, HS-F5/silica particles, normal-phase silica particles, C-1/silica particles, C-4/silica particles, C-6/silica particles, C-8/silica particles, C-30/silica particles, phenyl/silica particles, cyano/silica particles, ionic liquid/silica particles, molecular imprinted polymer particles, carboxen particles, divinylbenzene particles, diol/silica particles and mixtures thereof. 
   
   
       3 . The biocompatible coating according to  claim 2 , wherein the SPME particles are selected from the group consisting of C-18/silica particles, RP-amide/silica particles and HS-F5/silica particles. 
   
   
       4 . The biocompatible coating according to  claim 1 , wherein the biocompatible polymer is selected from the group consisting of polyacrylonitrile (PAN), polyethylene glycol, polypyrrole, derivatised cellulose, polysulfone and polyamide. 
   
   
       5 . The biocompatible coating according to  claim 4 , wherein the biocompatible polymer is polyacrylonitrile (PAN). 
   
   
       6 . The biocompatible coating according to  claim 1 , wherein the SPME particles are about 1.7 μm to about 50 μm particles. 
   
   
       7 . The biocompatible coating according to  claim 1 , wherein the SPME particles have a pore size from about 10 Å to about 200 Å. 
   
   
       8 . The biocompatible coating according to  claim 7 , wherein the SPME particles have a pore size from about 80 Å to about 180 Å. 
   
   
       9 . The biocompatible coating according to  claim 1 , wherein the SPME particles have a surface area of about 200 m 2 /g to about 800 m 2 /g. 
   
   
       10 . The biocompatible coating according to  claim 1 , wherein the polymer is a co-polymer of polyacrylonitrile. 
   
   
       11 . The biocompatible coating according to  claim 1 , wherein the matrix is selected from the group consisting of biological fluid, tissues, organs and cells. 
   
   
       12 . The biocompatible coating according to  claim 11 , wherein the biological fluid is whole blood, plasma, serum, urine, cerebrospinal fluid, saliva or peritoneal fluid. 
   
   
       13 . The biocompatible coating according to  claim 1 , wherein the small molecule is a drug or a biomarker. 
   
   
       14 . The biocompatible coating according to  claim 12 , wherein the drug is a hydrophobic or hydrophilic molecule having a molecular mass less than about 10,000 atomic mass units. 
   
   
       15 . A process for coating a flexible fiber with a biocompatible coating, the process comprising the steps of:
 coating the fiber with a suspension of solid phase microextraction (SPME) particles, the SPME particles being suspended in a solution of a biocompatible polymer and a solvent;   drying the coated fiber to remove the solvent; and   curing the dried coated fiber at an elevated temperature.   
   
   
       16 . The biocompatible coating according to  claim 15 , wherein the SPME particles are selected from the group consisting of C-18/silica particles, RP-amide/silica particles, HS-F5/silica particles, normal-phase silica particles, C-1/silica particles, C-4/silica particles, C-6/silica particles, C-8/silica particles, C-30/silica particles, phenyl/silica particles, cyano/silica particles, ionic liquid/silica particles, molecular imprinted polymer particles, carboxen particles, divinylbenzene particles, diol/silica particles and mixtures thereof. 
   
   
       17 . The biocompatible coating according to  claim 16 , wherein the SPME particles are selected from the group consisting of C-18/silica particles, RP-amide/silica particles and HS-F5/silica particles. 
   
   
       18 . The biocompatible coating according to  claim 15 , wherein the biocompatible polymer is selected from the group consisting of polyacrylonitrile (PAN), polyethylene glycol, polypyrrole, derivatised cellulose, polysulfone and polyamide. 
   
   
       19 . The biocompatible coating according to  claim 18 , wherein the biocompatible polymer is polyacrylonitrile (PAN). 
   
   
       20 . The process according to  claim 15 , wherein the solvent is selected from the group consisting of: dimethylformamide (DMF), dimethyl sulfoxide, NaSCN, Ca(CNS) 2 , nitric acid, ethylene carbonate and mixtures thereof. 
   
   
       21 . The process according to  claim 20 , wherein the solvent is dimethylformamide (DMF). 
   
   
       22 . The process according to  claim 15 , wherein the solution comprises the biocompatible polymer and the solvent being in a ratio of between about 5% and 15% biocompatible polymer/solvent (w/w). 
   
   
       23 . The process according to  claim 22 , wherein the biocompatible polymer/solvent ratio is between about 7.5% and 12% (w/w). 
   
   
       24 . The process according to  claim 23 , wherein the biocompatible polymer/solvent ratio is about 10% (w/w). 
   
   
       25 . The process according to  claim 15 , wherein the suspension comprises SPME particles and biocompatible polymer being in a ratio of about 0.3 and about 0.7 PAN/silica (w/w). 
   
   
       26 . The process according to  claim 25 , wherein the PAN/silica ratio is about 0.5 (w/w). 
   
   
       27 . The process according to  claim 15 , wherein the drying step comprises drying the coated fiber under flowing nitrogen. 
   
   
       28 . The process according to  claim 15 , wherein the elevated temperature is about 180° C. to about 210° C., and the curing step comprises maintaining the fiber at the elevated temperature for about 5 seconds to about 1.5 minutes. 
   
   
       29 . The process according to  claim 15 , wherein the coating, drying and curing steps are repeated at least once. 
   
   
       30 . The process according to  claim 15 , wherein the process is a continuous process. 
   
   
       31 . The process according to  claim 15 , wherein the process is a batch process. 
   
   
       32 . The process according to  claim 15 , wherein the fiber is a metal wire. 
   
   
       33 . The process according to  claim 32 , wherein the metal wire is stainless steel, titanium, a nickel-titanium alloy. 
   
   
       34 . The process according to  claim 33 , wherein the nickel-titanium alloy is Nitinol. 
   
   
       35 . A fiber coated with the biocompatible coating as defined in  claim 1 . 
   
   
       36 . A device for solid phase microextraction of a small molecule from a matrix, the device comprising the fiber as defined in  claim 35 . 
   
   
       37 . Use of the fiber as defined in  claim 35  for the solid phase microextraction of a small molecule from a matrix. 
   
   
       38 . A fiber coated according to the process of  claim 15 . 
   
   
       39 . A device for solid phase microextraction of a small molecule from a matrix, the device comprising the fiber according to  claim 38 . 
   
   
       40 . Use of the fiber as defined in  claim 38  for the solid phase microextraction of a small molecule from a matrix.

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