Decontamination solution and its use for denaturation, modification, degradation, solubilisation and removal of proteins, nucleic acid molecules and microorganisms

Abstract

The invention concerns a three component system comprising surface-active substances, vitamins and metal ions for efficient destruction and removal of contaminating proteins, nucleic acids and microorganisms from surfaces like for example laboratory benches, floors, equipment and instruments. These non-corrosive and non-toxic solutions for removal of proteins, nucleic acids and microorganisms are applied by spraying, rubbing or immersion of contaminated surfaces thereby destroying, solubilizing inactivating and removing proteins and nucleic acids. In that way also microorganisms are killed with high efficiency and at the same time all genetic information is inactivated.

1 . Decontamination solution comprising a synergistic mixture of
 a) at least one vitamin in concentrations from 1 mM to 1000 mM, and   b) at least one metal ion in concentrations of 1 mM to 100 mM, and   c) at least on surface-active substance in concentrations of 0.1% to 10% (weight) in relation to the total solution.   
   
   
       2 . Decontamination solution according to  claim 1 , wherein the mixture has a pH value ranging between pH 2 and pH 8.5. 
   
   
       3 . Decontamination solution according to  claim 1 , wherein the mixture additionally comprises a buffer system including carbonates and derivatives of succinic acid. 
   
   
       4 . Decontamination solution according to  claim 1 , wherein said vitamins, their related salts or acidic derivates are at least one compound selected from the group of the water-soluble vitamins having the characteristics of an antioxidant. 
   
   
       5 . Decontamination solution according to  claim 1 , wherein the metal ions are taken from metals from 4th group and sub-groups I, II, or VIII of the periodic table of the elements. 
   
   
       6 . Decontamination solution according to  claim 5 , wherein the metals are provided as salts of their acids or bases. 
   
   
       7 . Decontamination solution according to  claim 1 , wherein said surface-active substance is at least one compound of the group of the anionic, non-ionic, amphometeric or cationic tensides or suitable mixtures thereof. 
   
   
       8 .- 10 . (canceled) 
   
   
       11 . Method for denaturation, modification, degradation, solubilisation and removal of proteins, nucleic acid molecules and microorganisms from surfaces comprising
 providing the decontamination solution according to  claim 1  and treating surfaces for denaturation, modification, degradation, solubilisation and removal of proteins, nucleic acid molecules and microorganisms from said surfaces.   
   
   
       12 . Buffer system for adjusting the pH value of a solution, comprising carbonates and derivatives of succinic acid. 
   
   
       13 . Buffer system according to  claim 12 , characterized in that said carbonates and derivatives of succinic acid are each provided in concentrations between 1 mM and 500 mM. 
   
   
       14 . Buffer system according to  claim 12  wherein said solution is adjusted by the buffer system to a pH value ranging from pH 2 to 8.5. 
   
   
       15 . Method for adjusting the pH value of a solution which comprises at least a mixture of
 a) at least one vitamin, and   b) at least one metal ion, and   c) at least one surface-active substance,   wherein said mixture is adjusted by the buffer system comprising carbonates and derivatives of succinic acid to a pH value ranging from 2 to 8.5.   
   
   
       16 . Decontamination solution according to  claim 2 , wherein the mixture has a pH value ranging between pH 3 and 7. 
   
   
       17 . Decontamination solution according to  claim 2 , wherein the mixture has a pH value ranging between pH 4 and 6. 
   
   
       18 . Decontamination solution according to  claim 3 , wherein said carbonates and derivatives of succinic acid are each present in-a concentration between 1 mM and 500 mM. 
   
   
       19 . Buffer system according to  claim 14 , wherein said solution is adjusted by the buffer system to a pH value ranging from pH 3 to 7 
   
   
       20 . Buffer system according to  claim 14 , wherein said solution is adjusted by the buffer system to a pH value ranging from pH 4 to 6 
   
   
       21 . The method of  claim 15 , wherein said mixture is adjusted to a pH value ranging from 3 to 7. 
   
   
       22 . The method of  claim 15 , wherein said mixture is adjusted to a pH value ranging from 4 to 6.