US2009030185A1PendingUtilityA1

Synthetic genes for plant gums and other hydroxyproline-rich glycoproteins

61
Assignee: UNIV OHIOPriority: Jul 21, 1997Filed: May 15, 2008Published: Jan 29, 2009
Est. expiryJul 21, 2017(expired)· nominal 20-yr term from priority
Inventors:Marcia J. Kieliszewski
C07K 14/415C07K 2319/00C12N 15/8241C12N 15/8242
61
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Claims

Abstract

A new approach in the field of plant gums is described which presents a new solution to the production of hydroxyproline(Hyp)-rich glycoproteins (HRGPs), repetitive proline-rich proteins (RPRPs) and arabino-galactan proteins (AGPs). The expression of synthetic genes designed from repetitive peptide sequences of such glycoproteins, including the peptide sequences of gum arabic glycoprotein (GAGP), is taught in host cells, including plant host cells.

Claims

exact text as granted — not AI-modified
1 - 26 . (canceled) 
     
     
         27 . An isolated polynucleotide encoding an arabinogalactan protein. 
     
     
         28 . The polynucleotide according to  claim 27 , wherein the arabinogalactan protein comprises repeating modules. 
     
     
         29 . The polynucleotide according to  claim 28 , wherein the repeating modules are selected from the group consisting of (Ser-Pro) n  (SEQ ID NO: 85), (Ala-Pro) n  (SEQ ID NO: 86), and (Thr-Pro) n  (SEQ ID NO:87), and wherein n is from 7 to 50. 
     
     
         30 . The polynucleotide according to  claim 29 , wherein the repeating modules are selected from the group consisting of (Ser-Pro) n  (SEQ ID NO: 88), (Ala-Pro) n  (SEQ ID NO: 89), and (Thr-Pro) n  (SEQ ID NO: 90), and wherein n is 7, 20, 30, or 50. 
     
     
         31 . The polynucleotide according to  claim 30 , wherein the repeating module is (Ser-Pro) n  (SEQ ID NO: 88), and wherein n is 7, 20, 30, or 50. 
     
     
         32 . The polynucleotide according to  claim 30 , wherein the repeating module is (Ala-Pro) n  (SEQ ID NO: 89), and wherein n is 7, 20, 30, or 50. 
     
     
         33 . The polynucleotide according to  claim 30 , wherein the repeating module is (Thr-Pro) n  (SEQ ID NO: 90), and wherein n is 7, 20, 30, or 50. 
     
     
         34 . The polynucleotide according to  claim 27 , further encoding a non-gum arabic glycoprotein. 
     
     
         35 . The polynucleotide according to  claim 34 , wherein the arabinogalactan protein comprises repeating modules selected from the group consisting of (Ser-Pro) n  (SEQ ID NO: 85), (Ala-Pro) n  (SEQ ID NO: 86), and (Thr-Pro) n  (SEQ ID NO: 87), and wherein n is from 7 to 50. 
     
     
         36 . The polynucleotide according to  claim 35 , wherein the repeating module is (Ser-Pro) n  (SEQ ID NO: 88), and wherein n is 7, 20, 30, or 50. 
     
     
         37 . An isolated polypeptide comprising an arabinogalactan protein. 
     
     
         38 . The polypeptide according to  claim 37 , wherein the arabinogalactan protein comprises repeating modules. 
     
     
         39 . The polypeptide according to  claim 38 , wherein the repeating modules are selected from the group consisting of (Ser-Pro) n  (SEQ ID NO: 85), (Ala-Pro) n  (SEQ ID NO: 86), and (Thr-Pro) n  (SEQ ID NO: 87), and wherein n is from 7 to 50. 
     
     
         40 . The polypeptide according to  claim 39 , wherein the repeating modules are selected from the group consisting of (Ser-Pro) n  (SEQ ID NO: 88), (Ala-Pro) n  (SEQ ID NO: 89), and (Thr-Pro) n  (SEQ ID NO: 90), and wherein n is 7, 20, 30, or 50. 
     
     
         41 . The polypeptide according to  claim 40 , wherein the repeating module is (Ser-Pro) n  (SEQ ID NO: 88), and wherein n is 7, 20, 30, or 50. 
     
     
         42 . The polypeptide according to  claim 40 , wherein the repeating module is (Ala-Pro) n  (SEQ ID NO: 89), and wherein n is 7, 20, 30, or 50. 
     
     
         43 . The polypeptide according to  claim 40 , wherein the repeating module is (Thr-Pro) n  (SEQ ID NO: 90), and wherein n is 7, 20, 30, or 50. 
     
     
         44 . The polypeptide according to  claim 37 , further comprising a non-gum arabic glycoprotein. 
     
     
         45 . The polypeptide according to  claim 44 , wherein the arabinogalactan protein comprises repeating modules selected from the group consisting of (Ser-Pro) n  (SEQ ID NO: 85), (Ala-Pro) n  (SEQ ID NO: 86), and (Thr-Pro) n  (SEQ ID NO: 87), and wherein n is from 7 to 50. 
     
     
         46 . The polypeptide according to  claim 45 , wherein the repeating module is (Ser-Pro) n  (SEQ ID NO: 88), and wherein n is 7, 20, 30, or 50.

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