Methods and Materials Relating to CD84-like Polypeptides and Polynucleotides

Abstract

The invention provides novel polynucleotides and polypeptides encoded by such polynucleotides and mutants or variants thereof that correspond to a novel human secreted CD84-like polypeptide. These polynucleotides comprise nucleic acid sequences isolated from cDNA library from human spleen (Hyseq clone identification numbers 2938352 (SEQ ID NO: 1)). Other aspects of the invention include vectors containing processes for producing novel human secreted CD84-like polypeptides, and antibodies specific for such polypeptides.

1 . An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 2-3, 5, the translated protein coding portion thereof or the mature protein coding portion thereof. 
     
     
         2 . An isolated polynucleotide encoding a polypeptide with biological activity, which polynucleotide hybridizes to the complement of a polynucleotide of  claim 1  under stringent hybridization conditions. 
     
     
         3 . An isolated polynucleotide encoding a polypeptide with biological activity, said polynucleotide having greater than about 90% sequence identity with the polynucleotide of  claim 1 . 
     
     
         4 . The polynucleotide of  claim 1 , wherein said polynucleotide comprises the nucleotide sequence of SEQ ID NO: 3, or the mature protein coding portion thereof. 
     
     
         5 . The polynucleotide of  claim 1 , wherein said polynucleotide comprises the nucleotide sequence of SEQ ID NO: 5, or the mature protein coding portion thereof. 
     
     
         6 . The polynucleotide of  claim 3 , wherein said polynucleotide encodes a polypeptide comprising an amino acid sequence with at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 4. 
     
     
         7 . The polynucleotide of  claim 3 , wherein said polynucleotide encodes a polypeptide comprising an amino acid sequence with at least 99% sequence identity to the amino acid sequence of SEQ ID NO: 4. 
     
     
         8 . The polynucleotide of  claim 3 , wherein said polynucleotide encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 4. 
     
     
         9 . The polynucleotide of  claim 1  which is a DNA sequence. 
     
     
         10 . An isolated polynucleotide which comprises the complement of the polynucleotide of  claim 1 . 
     
     
         11 . A vector comprising the polynucleotide of  claim 1 . 
     
     
         12 . An expression vector comprising the polynucleotide of  claim 1 . 
     
     
         13 . A host cell genetically engineered to express the polynucleotide of  claim 1 . 
     
     
         14 . The host cell of  claim 13  wherein the polynucleotide is in operative association with a regulatory sequence that controls expression of the polynucleotide in the host cell. 
     
     
         15 . A method for detecting the polynucleotide of  claim 1  in a sample, comprising:
 a) contacting the sample with a compound that binds to and forms a complex with the polynucleotide of  claim 1  for a period sufficient to form the complex; and   b) detecting the complex, so that if a complex is detected, the polynucleotide of  claim 1  is detected.   
     
     
         16 . A method for detecting the polynucleotide of  claim 1  in a sample, comprising:
 a) contacting the sample under stringent hybridization conditions with nucleic acid primers that anneal to the polynucleotide of  claim 1  under such conditions;   b) amplifying a product comprising at least a portion of the polynucleotide of  claim 1 ; and   c) detecting said product and thereby the polynucleotide of  claim 1  in the sample.   
     
     
         17 . The method of  claim 16 , wherein the polynucleotide comprises an RNA molecule and the method further comprises reverse transcribing an annealed RNA molecule into a cDNA polynucleotide. 
     
     
         18 . A nucleic acid array comprising the polynucleotide of  claim 1  or a unique segment of the polynucleotide of  claim 1  attached to a surface. 
     
     
         19 . The array of  claim 18 , wherein the array detects full-matches to the polynucleotide or a unique segment of the polynucleotide of  claim 1 . 
     
     
         20 . The array of  claim 18 , wherein the array detects mismatches to the polynucleotide or a unique segment of the polynucleotide of  claim 1 .