Method of detecting mild impaired glucose tolerance or insulin secretory defect

Abstract

It is intended to provide a noninvasive method of conveniently detecting mild impaired glucose tolerance and/or insulin hyposecretion at the early stage with the use of an enzyme. Namely, mild impaired glucose tolerance and/or insulin hyposecretion at the early stage are detected by quantifying myoinositol secreted into the urine before loading glucose and after loading glucose for a definite period of time with the use of a reagent and comparing the increase (or the increase ratio) in the myoinositol content thus measured with a characteristic level which has been preliminarily determined in normal subjects.

1 - 14 . (canceled) 
   
   
       15 . A method of eliminating glucose in a sample, characterized in that two kinds of kinases are simultaneously used for the reaction of eliminating glucose in the sample. 
   
   
       16 . The method of eliminating glucose according to  claim 15 , characterized in that said two kinds of kinases are ATP-hexokinase and an ADP eliminating agent. 
   
   
       17 . The method of eliminating glucose according to  claim 16 , wherein the ADP eliminating agent is ADP-hexokinase. 
   
   
       18 - 26 . (canceled)