Human Chemotactic Cytokine I Polypeptides

Abstract

Human chemotactic cytokine I polypeptides and DNA (RNA) encoding such chemotactic cytokines and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such chemotactic cytokines for the treatment of leukemia, tumors, chronic infections, auto-immune disease, fibrotic disorders, wound healing and psoriasis. Antagonists against such chemotactic cytokines and their use as a therapeutic to treat rheumatoid arthritis, auto-immune and chronic and acute inflammatory and infective diseases, allergic reactions, prostaglandin-independent fever and bone marrow failure are also disclosed. Also disclosed are diagnostic assays for detecting diseases related to mutations in the nucleic acid sequences and altered concentrations of the polypeptides. Also disclosed are diagnostic assays for detecting mutations in the polynucleotides encoding the chemotactic cytokines and for detecting altered levels of the polypeptide in a host.

1 . An isolated polypeptide consisting of at least 30 contiguous amino acid residues of SEQ ID NO:2. 
     
     
         2 . The isolated polypeptide of  claim 1 , wherein said polypeptide consists of at least 50 contiguous amino acid residues of SEQ ID NO:2. 
     
     
         3 . An isolated polypeptide comprising amino acids 2 to 92 of SEQ ID NO:2. 
     
     
         4 . The isolated polypeptide of  claim 3 , wherein said polypeptide comprises amino acids 1 to 92 of SEQ ID NO:2. 
     
     
         5 . The isolated polypeptide of  claim 1  fused to a heterologous polypeptide. 
     
     
         6 . An isolated polypeptide consisting of at least 30 contiguous amino acid residues of the polypeptide encoded by the human cDNA contained in ATCC™ Deposit No. 97304. 
     
     
         7 . The isolated polypeptide of  claim 6 , wherein said polypeptide consists of at least 50 contiguous amino acid residues of the polypeptide encoded by the human cDNA contained in ATCC™ Deposit No. 97304. 
     
     
         8 . An isolated polypeptide comprising the mature polypeptide encoded by the human cDNA contained in ATCC™ Deposit No. 97304. 
     
     
         9 . An isolated polypeptide comprising the full length polypeptide encoded by the human cDNA contained in ATCC™ Deposit No. 97304. 
     
     
         10 . The isolated polypeptide of  claim 6  fused to a heterologous polypeptide. 
     
     
         11 . An isolated protein produced by the method comprising:
 (a) culturing a host cell under conditions suitable to produce the protein molecule of  claim 1 , and   (b) recovering said protein molecule.   
     
     
         12 . An isolated protein produced by the method comprising:
 (a) culturing a host cell under conditions suitable to produce the protein molecule of  claim 2 ; and   (b) recovering said protein molecule.   
     
     
         13 . An isolated protein produced by the method comprising:
 (a) culturing a host cell under conditions suitable to produce the protein molecule of  claim 3 ; and   (b) recovering said protein molecule.   
     
     
         14 . An isolated protein produced by the method comprising:
 (a) culturing a host cell under conditions suitable to produce the protein molecule of  claim 4 ; and   (b) recovering said protein molecule.   
     
     
         15 . An isolated protein produced by the method comprising:
 (a) culturing a host cell under conditions suitable to produce the protein molecule of  claim 5 , and   (b) recovering said protein molecule.   
     
     
         16 . An isolated protein produced by the method comprising:
 (a) culturing a host cell under conditions suitable to produce the protein molecule of  claim 6 ; and   (b) recovering said protein molecule.   
     
     
         17 . An isolated protein produced by the method comprising:
 (a) culturing a host cell under conditions suitable to produce the protein molecule of  claim 7 ; and   (b) recovering said protein molecule.   
     
     
         18 . An isolated protein produced by the method comprising:
 (a) culturing a host cell under conditions suitable to produce the protein molecule of  claim 8 ; and   (b) recovering said protein molecule.   
     
     
         19 . An isolated protein produced by the method comprising:
 (a) culturing a host cell under conditions suitable to produce the protein molecule of  claim 9 , and   (b) recovering said protein molecule.   
     
     
         20 . An isolated protein produced by the method comprising:
 (a) culturing a host cell under conditions suitable to produce the protein molecule of  claim 10 , and   (b) recovering said protein molecule.