Soybean mutant strain and soybean oil therefrom

Abstract

A soybean oil of a decreased content of polyunsaturated fatty acids is produced by hybridizing, selecting and raising a soybean mutant KK21 characterized by a gene having a sequence of base given by the sequence No. 1 of the sequence listing, a soybean mutant M23 characterized by deletion of a gene having a sequence of base given by the sequence No. 2 of the sequence listing, a soybean mutant M24 characterized by a gene having a sequence of base given by the sequence No. 3 of the sequence listing, a soybean mutant M5 characterized by a gene having a sequence of base given by the sequence No. 4 of the sequence listing, a soybean mutant J18 characterized by deletion of a gene having a sequence of base given by the sequence No. 5 of the sequence listing, and one or two kinds or more of these soybean mutants.

1 . A soybean mutant strain capable of fruiting beans of a decreased content of polyunsaturated fatty acids, wherein the mutant comprises a genome lacking the nucleotide sequence of SEQ ID NO: 2 or SEQ ID NO: 5. 
     
     
         2 . A soybean oil of a decreased content of polyunsaturated fatty acids as an oleaginous ingredient in the beans of the soybean mutant strain defined in  claim 1 . 
     
     
         3 . A method for assay of a mutant gene derived from the mutant strain KK21 which comprises the steps of:
 amplifying a part of the base sequence of the gene GmFAD2-1 by the PCR reaction using the primer 1: 5′-attgatagcccctccgttcccaaga-3′ (SEQ ID NO: 6) and the primer 2: 5′-attgtgagtgtgacgagaagagaaac-3′ (SEQ ID NO: 7); and   analyzing for the target base sequence by using the primer 3: 5′-gggtctagcaaaggaaacaacaatgggaggt-3′ (SEQ ID NO: 8).   
     
     
         4 . (canceled) 
     
     
         5 . A method for assay of a mutant gene derived from the mutant strain J18 which comprises the steps of:
 amplifying simultaneously a part of the base sequence of the gene GmFAD3-1a and a part of the base sequence of the gene GmFAD3-1b by the PCR reaction using the primer 5: 5′-ttattacgcaccacccaccacgtatccct-3′ (SEQ ID NO: 10) and the primer 6: 5′-gttgcgagtggaggagcagagaatcagtc-3′ (SEQ ID NO: 11); and   analyzing for the target base sequence by a combination of digestion with the restriction enzyme Kpn I and the method of electrophoresis.   
     
     
         6 . A method for assay of the mutant gene derived from the mutant strain M5 or the mutant strain derived from the mutant strain M24 which comprises the steps of:
 amplifying simultaneously a part of the base sequence of the gene GmFAD3-1a and a part of the base sequence of the gene GmFAD3-1b by the PCR reaction using the primer 5: 5′-ttattacgcaccacccaccacgtatccct-3′ (SEQ ID NO: 10) and the primer 6: 5′-gttgcgagtggaggagcagagaatcagtc-3′ (SEQ ID NO: 11);   conducting separation of the target base sequences by a combination of digestion with the restriction enzyme of Kpn I and the method of electrophoresis; and   analyzing for the base sequence by using the primer 7: 5′-gtggatcgtgactatggttggatcta-3′ (SEQ ID NO: 12).   
     
     
         7 . A soybean mutant strain capable of fruiting beans of a decreased content of polyunsaturated fatty acids, wherein the mutant comprises a genome having a nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 4.