Human serum for cell culture

Abstract

It is intended to provide a serum which contains a large amount of growth factors capable of efficiently promoting the growth of stem cells. A human serum for cell culture which shows a residual ratio of platelets remaining within 20 minutes after blood collection in relation to the whole amount of the platelets is 0% to 20%, and a release ratio of cell growth factors is 20% to 100%.

1 . A human serum for cell culture obtained from a fluid comprising humoral components derived from blood having blood coagulation factors and platelets, and wherein
 a residual ratio of the platelets remaining within 20 minutes after blood collection in relation to the whole amount of the platelets is 0% to 20%, and a release ratio of cell growth factors is 20% to 100%.   
   
   
       2 . A human serum for cell culture obtained from a fluid comprising humoral components derived from blood having blood coagulation factors and platelets, and wherein
 a content of the cell growth factors is greater than that of a human serum prepared from plasma.   
   
   
       3 . A human serum for cell culture obtained from a fluid comprising humoral components derived from blood having blood coagulation factors and platelets, and wherein
 a content of the cell growth factors is greater than that of a human serum prepared by allowing the blood to coagulate.   
   
   
       4 . The human serum for cell culture according to  claim 1 , wherein
 the cell growth factors comprise at least one of PDGF-BB and TGF-βf1.   
   
   
       5 . The human serum for cell culture according to  claim 1 , wherein
 the cell growth factors are obtained by bringing the fluid into contact with a processed glass body.   
   
   
       6 . The human serum for cell culture according to  claim 5 , wherein
 the processed glass body is comprised of glass beads.   
   
   
       7 . The human serum for cell culture according to  claim 1 , wherein
 the human serum is prepared without being exposed to the atmosphere.   
   
   
       8 . The human serum for cell culture according to  claim 1 , wherein
 the human serum is usable for a regenerative medicine method.